Immunogen: Synthetic peptide from the C-terminal region of human protein Nrf2
Species Reactivity: (+) Human, mouse, and rat Nrf2
Application(s): FC, IF, and WB
This C-terminal Nrf2 polyclonal antibody preferentially detects polyubiquitinated Nrf2 at 90 kDa. Nrf2 forms a heterodimer with a small Maf protein and binds to the antioxidant response element in the upstream promoter region of many antioxidative genes. Under normal, unstressed conditions, Nrf2 is sequestered in the cytoplasm where it is bound by Keap1 and cullin 3. Certain stressors disrupt Keap1 binding and prevent ubiquitination, leading to subsequent translocation into the nucleus.