Product is rabbit affinity purified antibody to bovine albumin and buffer salts.
Preparation Method
The affinity purified antibody is prepared from the specific rabbit IgG fraction by solid phase absorption. The resulting affinity purified antibody is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a 0.22 µm filter, and vialed. No preservative has been added.
Analysis Note
The total protein is measured by absorbance (A280nm). Antibody titer is standardized by equivalence-point precipitation. Each affinity purified antibody is tested for purity at 10 mg/mL using immunoelectrophoresis. The product is rabbit IgG; no trace of albumin is detected.