Product is the lyophilized powder of goat IgG fraction to chicken egg albumin and buffer salts.
Goat IgG Fraction to Chicken Egg Albumin is suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. It is also used in conjugation with enzymes, radiolabels, fluorochromes or other markers, and for attachment to solid supports for use as an immunoadsorbant. Used as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or tissue immunostaining.
Reconstitute product with 5.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material.
Typical Working Concentration
Suggested working dilution: Quantitative precipitin assay: 3-5 mg/mL
The IgG fraction is prepared from the specific goat antiserum by delipidation, ammonium sulfate fractionation and anion exchange chromatography. The resulting IgG fraction is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, 0.05% sodium azide added, adjusted to standard titer, filtered through a 0.22 µm filter, vialed and lyophilized.
The total protein is measured using the Biuret procedure, with bovine albumin as a standard. Antibody titer is standardized to an in-house standard by equivalence-point precipitation. Each IgG fraction is tested for purity and specificity at a minimum of 40 mg/mL using immunoelectrophoresis; No trace of albumin is detected. Antibody activity to serum albumin is not present.