Antigen: synthetic peptide containing amino acids near the 214/215 cleavage site of human PARP
Cross Reactivity: (+) human PARP
Application(s): FC (intracellular) and WB
PARP is a 116 kDa nuclear chromatin-associated enzyme that is cleaved during apoptosis by caspase-3 into a 24 kDa fragment containing the DNA binding domain and an 89 kDa fragment containing the catalytic and automodification domains. The 24 kDa fragment irreversibly binds to DNA and may contribute to the irreversibility of apoptosis by blocking the access of DNA repair enzymes to DNA strand breaks.