Actin Monoclonal Antibody was used for western blot analysis of Cos-7 cell lysates to ensure equal protein loading. Western blot analysis of MDCK cell lysates were performed using monoclonal anti-actin antibody as a primary antibody. Also It's used in Indirect ELISA, Immunofluorescence, Immunoblots and Immunohistochemistry.
The monoclonal actin (clone C4) is a mouse IgG1 (gamma 1 heavy/kappa light chain) antibody.
Indirect Immunofluorescence: 1:500 Immunoblots: 1:500 for DAB/peroxidase detection, actin band migrates at 42 kDa on SDS-PAGE. Immunohistochemistry: 1:500
Application Areas: Immunoassays
Research Areas: Cell Structure
Product Type: Antibodies
Antibody Type: Primary Antibodies
Species Reactivity: Mammals, yeast, fungi
NOTES: This product is presented as filtered ascites containing 0.01% sodium azide as a preservative.
Storage & Handling: Store at -20°C. Storage at -70°C will give a longer storage time. Stable for weeks at 2-8°C. Avoid repeated freeze/thaw cycles.