Antigen: synthetic peptide containing phosphorylated serine residues corresponding to human IκBα amino acids 32 and 36
Host: mouse, clone 39A1413
Isotype: IgG1κ
Cross Reactivity: (+) bovine, canine, human, mouse, porcine, and rat IκBα
Application(s): WB and IP
External stimuli such as tumor necrosis factor or other cytokines initiates a signal transduction cascade that leads to activation of the IκB-kinase complex that specifically phosphorylates IκBα on serine32 and serine36. Phosphorylation of these sites leads to ubiquitination of IκBα and subsequent degradation by the 26S proteasome. Degradation of IκBα results in unmasking of the nuclear localization signal of NF-κB dimers, which subsequently translocate to the nucleus and activate target genes.