Anti-HA (HA.C5 / C10) Tag mAb, similar to Anti-His (HIS.H8) Tag mAb, has also high affinity and high specificity.
Anti-HA (HA.C5 / C10) Tag mAb, similar to Anti-His (HIS.H8) Tag mAb, has also high affinity and high specificity.
Description: Anti-HA Tag (HA.C5 / C10) Monoclonal Antibody
Clonality: HA.C5 / C10
Host Species: Mouse
Isotype: IgG3
Immunogen: YPYDVPDYA (HA) synthetic peptide conjugated to KLH
Specificity: Recognizes the N-terminal, C-terminal or internal HA-tagged fusion proteins
Purification: Protein A affinity chromatography from mouse ascites fluid
Applications: Dot, ELISA, IP, IS, WB (see notations)
Sensitivity: WB (with ECL): 1-5ng of HA-tagged fusion proteins
Form: 1mg/mL in 10mM PBS, pH 7.2
Working Conditions: WB (with ECL): 1:1000-3000 dilution (incubate for one hour at room temperature)
IS: 1:500-2000 dilution
For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test
Storage: Store at 4°C (add 0.1% NaN3) for several days to weeks, and at -20°C for longer periods. Avoid repeated freeze-thaw cycles.
Product Type : Tag Abs / Tag Antibodies / Tag Antibody