Storage: 2-8C
UNSPSC Code: 12352200
General description: Chemiluminescent immunoassay for the quantification of cell proliferation, based on the measurement of BrdU incorporation during DNA synthesis: -áa nonradioactive alternative to the [3H]-thymidine incorporation assay.
Application: The Cell Proliferation ELISA, BrdU (chemiluminescent) belongs to the second, improved generation of kits for measuring DNA synthesis. It is a precise, fast, and simple nonradioactive alternative to quantitate cell proliferation based on the measurement of BrdU incorporation during DNA synthesis in replicating (cycling) cells. Thus, the Cell Proliferation ELISA can be used in many different in vitro cell systems. For example:. Detection and quantification of cell proliferation induced by growth factors and cytokines. Determination of the inhibitory or stimulatory effects of various compounds on cell proliferation in environmental and biomedical research, and in the food, cosmetic, and pharmaceutical industries. Measurement of the immunoreactivity of lymphocytes, stimulated by mitogens or antigens. Analysis of the chemosensitivity of tumor cells to different cytostatic drugs in medical research
Specificity: The antibody conjugate reacts with the thymidine analogue 5-bromo-2'-deoxyuridine (BrdU) and with BrdU incorporated into DNA. For binding to BrdU incorporated into the DNA, the BrdU-labeled DNA has to be denatured. The antibody does not cross-react with any endogenous cellular components such as thymidine, uridine, or DNA.
Other Notes: For life science research only. Not for use in diagnostic procedures.
Components: BrdU Labeling Reagent; FixDenat ready-to-use; Anti-BrdU-peroxidase antibody; Antibody Dilution Solution ready-to-use; ready-to-use 10x concentrated; Substrate Component A; Substrate Component B
RIDADR: UN1170 - class 3 - PG 2 - Ethanol, solution