Protein A is a cell-wall receptor which is present on cell wall of various starins of Staphyloccocus aureus. Protein A contains four high-affinity binding sites for interacting with the Fc region of immunoglobulin Gs of various species including mouse, rabbit and human. Protein A is used for making probes for detection of antibodies and for making affinity media for purification of antibodies.
Protein A (Recombinant) is recombinant protein A expressed in E. coli. It functions similarly as the native Protein A.
Protein A (Recombinant) can be conjugated with reporter molecules, fluorescent dyes, enzyme markers, biotin or colloidal gold for detection of antibodies by immunochemical assays including ELISA, western blotting or Immunohistochemistry.
Protein A (Recombinant) can be immobilized on a solid support such as agarose to purify monoclonal or polyclonal antibodies.
Features:
- Source: E. coli.
- Molecular weight: ~47kDa; exact sequence as the native Protein A.
- Binds 3 IgG Isotypes.
- Purity: ≥ 95%, HPLC, Single major band .
- Form: Lyophilized powder.
Application(s):
- Protein A (Recombinant) conjugated to reporter molecules or fluourescent dyes or enzymemarkers can be used in detection of antibody by immunochemicals assays.
- Protein A (Recombinant)immobilized on a solid support such as agarose is usedto purify monoclonal or polyclonal antibodies.
Image By E A S (Own work)