Tissue-PE LB™ has been developed for extraction of total soluble protein from animal tissues.Tissue- PE LB™ is based on an organic buffer, which utilizes a mild non-ionic detergent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins.Depending on the application, additional agents such as chelating agents, reducing agents and protease inhibitors may be added into Tissue-PE LB™. Tissue- PE LB™ reagent has been tested for use with a wide variety of animal tissues, fresh as well as frozen tissues.
The protein extract prepared with Tissue-PE LB™ may be used for most enzyme assays including reporter gene assays (e.g. β-galactosidase, luciferace, chloramphenical acetytransferease), kinases (e.g. PKC, PKA, Tyrosin Kinase), and immunoassays (e.g., ELISA, Western blots, RIA).
COMPATIBILITY
Tissue-PE LB™ is compatible with most downstream applications including various enzyme assays, running various chromatography, and gel electrophoresis applications. Tissue-PE LB™ is also compatible for protein estimation with NI™ (Non-Interfering) Protein Assay .
Features
- Provides a simple and versatile method for protein extraction from fresh or frozen tissues.
- Compatible with most enzyme assays including:
- Reporter gene assays; ß-galactosidase, luciferase, chloramphenicol acetyltransferase.
- Kinases; Protein kinase C, protein kinase A, tyrosine kinase.
- Immunoassays; ELISA, Western blots, RIA.
- Compatible with electrophoresis and chromatographic applications.
Applications
- For extraction of soluble proteins from fresh and frozen animal tissue.
- Suitable for most applications including enzyme and protein purification applications, electrophoresis, Western blotting and 2D-gel analysis.