Insect Cell-PE LB™ has been developed for extraction of cytoplasmic soluble protein from insect cultured cells. The Insect Cell-PE LB™ is based on organic buffering agents, which utilizes a mild non-ionic detergent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the application, additional agents such as chelating agents, reducing agents and protease inhibitors may be added into Insect Cell-PE LB™.Insect Cell-PE LB™ can be used for both suspension as well as adherent cells. The proprietary combination of this reagent provides a simple and versatile method for the extraction of protein from insect cells.
COMPATIBILITY
Insect Cell-PE LB™ is compatible with most applications, including enzyme assays, various chromatography procedures, electrophoresis, etc. Insect Cell-PE LB™ is also compatible for protein estimation with NI™ protein assay(Non-Interfering Protein Assay™). The protein extract prepared with Insect Cell-PE LB™ may be used for most enzyme assays including reporter gene assays (e.g. β-galactosidase, luciferase, chloramphenicol acetyltransferase), kinases (e.g., PKC, PKA, Tyrosine Kinase), and immunoassays (e.g., ELISA, Western blots, RIA).
Features
- Provides a simple and versatile method for protein extraction from adherent or suspended Sf9 and Sf21 insect cells
- Compatible with electrophoresis and chromatographic applications
Applications
- For extraction of soluble proteins from cultured insect cells
- Suitable for most applications including enzyme and protein purification applications, electrophoresis, Western blotting and 2D-gel analysis