FOCUS™ Glycoprotein kit is based on lectin binding of specific glycoproteins with terminal α-D mannosyl and α-D glycosyl proteins. FOCUS™ Glycoprotein kit isolates glycoproteins from complex biological solutions using spin columns that contain lectin (Concanavalin A) bound resin with the capacity to bind and immobilize ~20mg glycoproteins. Column bound glycoproteins are eluted with a set of three rapid elution buffers. The proprietary serial elution allows for faster elution compared to other glycoprotein purification systems. The eluted proteins are suitable for 2D electrophoresis and isoelectric focusing.
Features
- Spin column protocol
- Uses a high capacity lectin binding resin (10-16mg/ml resin)
- Elution of glycoproteins within 90 minutes with a set of three rapid elution buffers
Applications
- Fractionation and enrichment of glycoproteins suitable for 2D eletrophoresis and other applications