The Pearl™ Monoclonal IgG Purification kit allows for the rapid purification of antibodies from cell culture supernatant and ascites fluid. The Pearl™ IgG Purification Resin binds the high abundant, non-IgG proteins (i.e. albumin) and allows the IgG molecules to pass through in a physiological buffer.The IgG molecules can be stored or used in downstream applications without further clean-up, such as ammonium sulfate precipitation.
The Pearl™ Monoclonal IgG Purification kit can be used to purify antibodies direct from cell culture supernatant with less than 10% FBS or can be used with ascites fluid after treatment with the supplied Ascites PreTreat.
The Pearl™ Monoclonal IgG Purification kit can purify IgG from ~1L cell culture supernatant or 200ml ascites fluid.
Also availlable are complete IgG Purification Kits , which are available in two formats:
A spin format that is ideal for the rapid, small scale purification of IgG. The kit is supplied with 3ml Pearl™ IgG Purification Resin, IgG Isolation Buffer and 20 spin columns. Suitable for purifying up to 25mg IgG.
A larger format kit is supplied with 25ml Pearl™ IgG Purification Resin and IgG Isolation Buffer and is suitable for the isolation of IgG from ~100ml serum (~200mg 1gG).
Features
- Isolate monoclonal antibodies for ascites fluid or cell culture supernatant
- Supplied with ascites pretreatment reagent for optimal IgG purification
- For 1L of cell culture supernatant or 0.2L ascites fluid
Applications
- Monoclonal antibody isolation from ascites fluid or cell culture supernatant