The Pearl™ IgG Purification kits are designed for the one step purification of IgG (Immunoglobulin G) antibodies from serum.The supplied Pearl™ IgG Purification Resin binds the high abundant, non-IgG proteins (i.e albumin) and allows the IgG molecules to pass through in a physiological buffer.The purified IgG molecules can be stored or used in further downstream applications without further clean-up, such as ammonium sulfate precipitation.
The Pearl™ IgG purification kits can purify IgG in <15 minutes, which is more rapid than the commonly used Protein A and Protein G resins.The performance of the Pearl™ IgG Purification Resin is comparable or better than the ProteinA and Protein G resins.
| Species | Pearl™ IgG Purification Resin | Protein A | Protein G |
| Mouse | ++++ | ++++ | ++++ |
| Human | ++++ | ++++ | ++++ |
| Rat | ++++ | + | ++ |
| Hamster | ++++ | ++ | ++ |
| Guinea Pig | ++++ | ++++ | ++ |
| Rabbit | ++++ | ++++ | +++ |
| Horse | ++++ | ++ | ++++ |
| Cow | ++ | ++ | ++++ |
| Pig | ++++ | +++ | ++ |
| Sheep | ++ | + | ++ |
| Goat | ++++ | + | ++ |
| Chicken | - | - | - |
The Pearl™ IgG Purification Kits are available in two formats:
Cat. # 786-798 is a spin format that is ideal for the rapid, small scale purification of IgG. The kit is supplied with 3ml Pearl™ IgG Purification Resin, IgG Isolation Buffer and 20 spin columns. Suitable for purifying up to 25mg IgG.
Cat. # 786-799 is supplied with 25ml Pearl™ IgG Purification Resin and IgG Isolation Buffer and is suitable for the isolation of IgG from ~100ml serum (~200mg IgG).
Also availlable is our Pearl™ Monoclonal IgG Purification Kit , which purifies monoclonal antibodies from ascites and cell culture supernatant.
Features
- Simple 1-step purification, eliminates Protein A & G binding and elution steps
- High recovery (>90%) & Purity (>80%)
- Purify IgG from sources not compatible with ProteinA & G
Applications
- Purification of IgG (Immunoglobulin G) molecules