G-Trap™ CM Agarose Fast Flow is ready to use prepacked column used for separation of proteins and other biomolecules based on charge. The resin in G-Trap™ CM Agarose Fast Flow is a weak cation exchanger composed of highly crosslinked 6% agarose beads coupled with carboxymethyl (CM) weak cation exchange group. The proteins carrying net possitive charge will bind to the resin and protein carrying net negative charge will not bind and will come out in flow through fraction.
There are otherG-Trap™ Ion Exchange Agarose Fast Flow Columns availablein 1 ml and 5 ml column volume namely G-Trap™ SP Agarose Fast Flow , G-Trap™ DEAE Agarose Fast Flow and G-Trap™ Q Agarose Fast Flow .G-Trap™ SP Agarose Fast Flow contains cation exchanger resin and G-Trap™ DEAE Agarose Fast Flow and G-Trap™ Q Agarose Fast Flow contains anion exchanger resin. G-Trap™ Ion Exchange Selection Kit is available for initial screening for the appropriate ion exchanger to purify target protein.
G-Trap™ Ion Exchange Agarose Fast Flow Columns are ready to use prepacked columns for separation of biomolecules on the basis of charge. G-Trap™ Ion Exchange Agarose Fast Flow Columns are used for method scouting, separation or purification of biomolecules, sample concentration and sample cleanup for charged molecules. Biomolecules that carry net charge opposite to that of ion-exchanger bind to that ion exchanger and are thus separated from bulk molecules in the sample.
Theproperties of theG-Trap™ CM Agarose Fast Flow resin and the column are listed below
G-Trap™ CM Agarose Fast Flow resin
Ion Exchanger:Weak cation exchanger
Matrix:Cross-linked agarose beads, 6%
Bead size:45-165 µm
Ligand :Carboxymethyl
Ionic Capacity:0.09-0.13 mmol (H+)/ml
Binding Capacity:50 mg lysozyme/ ml medium
pH stability Working Range:4-12
pH stability Cleaning-in-Place (CIP):2-14
Maximum Flow Velocity:450cm/h
Recommended Flow rate:1 ml/ min and 5 ml/ min for 1 ml and 5 ml column respectively
Maximum Pressure:0.3MPa
Exclusion limit(globular proteins):4 x 106
Chemical Stability:Stable to all commonly used aqueous buffers:1 M NaOH, 8M urea, 8M guanidine hydrochloride, 70% ethanol
Storage Conditions:4 to 30°C, 20% Ethanol
G-Trap™ CM Agarose Fast Flow?, 1 ml columns Column Volume: 1 ml
Columns Dimension: 0.7 x 2.5 cm
Column Harware Pressure Limit: 0.5 MPa
Column Hardware: Polypropylene (Biocompatible)
G-Trap™ CM Agarose Fast Flow?, 5ml columns
Column Volume: 5 ml
Columns Dimension: 1.6 x 2.5 cm
Column Harware Pressure Limit: 0.5 MPa
Column Hardware: Polypropylene (Biocompatible)
Application(s):
- G-Trap™ CM Agarose Fast Flow is ready to use prepacked column used for separation of proteins and other biomolecules based on charge.The resin in G-Trap™ CM Agarose Fast Flow is a weak cation exchangerwherecarboxymethyl (CM) weak cation exchange groupis coupled to agarose.The proteins carrying net possitive charge will bind to the resin and protein carrying net negative charge will not bind and will come out in flow through fraction.