G-Trap™ HIC Selection Kit consists of 4 prepacked 1 ml columns (G-Trap™ HIC Columns) with matrix or resin for hydrophobic interaction chromatography. The kit consists of 4 pepacked columns namely G-Trap™ Butyl Agarose 6 Fast Flow, G-Trap™ Octyl Agarose 6 Fast Flow, G-Trap™ Phenyl Agarose 6 Fast Flow (High Sub) and G-Trap™ Phenyl Agarose 6 Fast Flow (Low Sub).
G-Trap™ HIC Selection Kit allows for initial screening of appropriate ligand /matrix and conditions for isolation of a specific protein or biomolecule. Once appropriate resin/medium is determined, individual G-Trap™ HIC Column in 1 ml or 5 ml sizes can be used. Individual G-Trap™ HIC Columns are available at G-biosciences in 1 ml and 5 ml column volume size
The G-Trap™ HIC Columns available for seperataion of the target protein includes G-Trap™ Butyl Agarose 6 Fast Flow , G-Trap™ Octyl Agarose 6 Fast Flow , G-Trap™ Phenyl Agarose 6 Fast Flow (High Sub) and G-Trap™ Phenyl Agarose 6 Fast Flow (Low Sub).
Biomolecules are separated on the HIC column based upon the hydrophobicity of the ligand attached to the resin, the distribution of surface-exposed hydrophobic amino acids present on the protein or enzyme to be separated and the concentration and type of salt used in binding or start buffer.
Straight alkyl chains ligand s such as butyl, octyl show pure hydrophobic character whereas aryl ligand s such as phenyl show mixed mode behavior where aromatic, hydrophobic as well as lack of charge play role in protein/biomolecules adsorption to the column. In HIC, the binding of biomolecules to the column (ligand ) is facilitated by using higher concentrations of anti-chaotropic salts such as ammonium sulfate, sodium sulphate etc in the binding buffer.
The properties ofG-Trap™ HIC Selection Kit resin and column are listed below
G-Trap™ HIC Selection Kit resin
G-Trap™ Butyl Agarose 6 Fast Flow Resin
Mean particle size: 90 µm
Bead structure: Highly cross-linked 6% Agarose
Ligand : Butyl
Ligand Concentration: About 40μmol/ml
Binding Capacity: About 20mg HSA/ml resin
pH stability Working Range: 3-13
pH stability Cleaning-in-Place (CIP): 2-14
Maximum Pressure (MPa): 0.3
Maximum Flow Velocity: 450cm/h
Exclusion Limit (Globular Proteins): 4 x 106
Physical Stability: Negligible volume variation due to changes in pH or ionic strength
Chemical Stability: Stable to all commonly used aqueous buffers:1 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol
Storage Conditions: 4 to 30°C, 20% Ethanol
G-Trap™ Octyl Agarose 6 Fast Flow Resin
Mean particle size: 90 µm
Bead structure: Highly cross-linked 6% Agarose
Ligand : Octyl
Ligand Concentration: About 5μmol/ml
Binding Capacity: About 30mg HSA/ml resin
pH stability Working Range: 3-13
pH stability Cleaning-in-Place (CIP): 2-14
Maximum Pressure (MPa): 0.3
Maximum Flow Velocity: 450cm/h
Exclusion Limit (Globular Proteins): 4 x 106
Physical Stability: Negligible volume variation due to changes in pH or ionic strength
Chemical Stability: Stable to all commonly used aqueous buffers:1 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol
Storage Conditions: 4 to 30°C, 20% Ethanol
G-Trap™ Phenyl Agarose 6 Fast Flow (High Sub) Resin
Mean particle size: 90 µm
Bead structure: Highly cross-linked 6% Agarose
Ligand : Phenyl
Ligand Concentration: About 40μmol/ml
Binding Capacity: About 40mg HSA/ml resin
pH stability Working Range: 3-13
pH stability Cleaning-in-Place (CIP): 2-14
Maximum Pressure (MPa): 0.3
Maximum Flow Velocity: 450cm/h
Exclusion Limit (Globular Proteins): 4 x 106
Physical Stability: Negligible volume variation due to changes in pH or ionic strength
Chemical Stability: Stable to all commonly used aqueous buffers:1 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol
Storage Conditions: 4 to 30°C, 20% Ethanol
G-Trap™ Phenyl Agarose 6 Fast Flow (Low Sub) Resin
Mean particle size: 90 µm
Bead structure: Highly cross-linked 6% Agarose
Ligand : Phenyl
Ligand Concentration: About 25 μmol/ml
Binding Capacity: About 20 mg HSA/ml resin
pH stability Working Range: 3-13
pH stability Cleaning-in-Place (CIP): 2-14
Maximum Pressure (MPa): 0.3
Maximum Flow Velocity: 450 cm/h
Exclusion Limit (Globular Proteins): 4 x 106
Physical Stability: Negligible volume variation due to changes in pH or ionic strength
Chemical Stability: Stable to all commonly used aqueous buffers:1 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol
Storage Conditions: 4 to 30°C, 20% Ethanol
G-Trap™ HIC Selection Kit, 1 ml column
Column Volume: 1 ml
Columns Dimension: 0.7 x 2.5 cm
Column Harware Pressure Limit: 0.5 MPa
Column Hardware: Polypropylene (Biocompatible)
Application(s):
- G-Trap™ HIC Selection Kit allows for initial screening of appropriate ligand /matrix and conditions for isolation of a specific protein or biomolecule.The kit consists of 4 pepacked columns namely G-Trap™ Butyl Agarose 6 Fast Flow, G-Trap™ Octyl Agarose 6 Fast Flow, G-Trap™ Phenyl Agarose 6 Fast Flow (High Sub) and G-Trap™ Phenyl Agarose 6 Fast Flow (Low Sub).