G-Trap™ GT-100 Desalting Column is prepacked ready to use column for separation of low molecular weight substances from high molecular weight substances based on size exclusion chromatography.
G-Trap™ GT-100 Desalting Columns are mostly used for removal of salt or buffer exchange before or after different chromatographic steps. They can alsobe used for separation of proteins based on their sizes.
Other desalting columns available are G-Trap™ GT-600 Desalting Column and G-Trap™ GT-1200 Desalting Column in 1 ml and 5 ml Column Volume.
The properties of theG-Trap™ GT-100 Desalting Column resin and the column are listed below
G-Trap™ GT-100 Desalting Column resin
Bead size:55-165 µm
Void volume:~0.3 ml for 1 ml column and ~1.5 ml for 5 ml column
Sample Load volume:0.03 ml- 0.3 ml for 1 ml column and 0.75-1.5 ml for 5 ml column
Exclusion limit (Mr):700
Recommended Flow rate:1 ml/min for 1 m column and 5 ml/min for 5 ml column
Chemical stability:All commonly used buffers, 8M urea, 6M guanidine hydrochloride and all non-ionic detergents. Lower alcohols such as methanol, ethanol and propanol can be added to buffers at lower than 25 V/V %
pH stability:2 to 13
Storage:20 % ethanol at 4°C t 30°C
?G-Trap™ GT-100 Desalting Column, 1 ml column
Column Volume: 1 ml
Columns Dimension: 0.7 x 2.5 cm
Column Harware Pressure Limit: 0.5 MPa
Column Hardware: Polypropylene (Biocompatible)
?G-Trap™ GT-100 Desalting Column, 5ml column
Column Volume: 5 ml
Columns Dimension: 1.6 x 2.5 cm
Column Harware Pressure Limit: 0.5 MPa
Column Hardware: Polypropylene (Biocompatible)
Application(s):
- G-Trap™ GT-100 Desalting Column is prepacked ready to use column for separation of low molecular weight substances from high molecular weight substances based on size exclusion chromatography.G-Trap™ GT-100 Desalting Columns are mostly used for removal of salt or buffer exchange before or after different chromatographic steps. They can alsobe used for separation of proteins based on their sizes.