G-Trap™ rProtein A FF are prepacked ready to use columns for purification of IgG antibodies.
The column is packed with fast flow resin, which is covalently coupled to recombinant Protein A. Recombinant Protein A is coupled covalently by proprietary coupling method to highly crosslinked 6% agarose.
The recombinant Protein A, produced in E. coli, has been genetically engineered to enhance its binding capacity to the antibodies
G-Trap™ Protein G, 1 ml and G-Trap™ Protein G, 5 ml are also available for affinity purification of Immunoglobulins G.
The key characteristics of the recombinant Protein A resin or matrix used in G-Trap™ rProtein A FFand the column properties are listed below
G-Trap™ rProtein A resin
- Mean particle size:90 µM
- Bead Structure:Highly cross-linked 6% agarose
- Ligand : Recombinant Staphylococcal Protein A from E.coli
- Binding capacity:>40 mg Human IgG/ ml resin
- Recommended working flow rate:1 ml/min and 5 ml/min for 1 ml and 5 ml column respectively.
- Chemical Stability:Stable in all commonly used buffers
- pH stability:Long term: pH 3-9, short term pH 2-9
- Storage:2°C to 8°C in 20% ethanol
G-Trap™ rProtein A, 1 ml columns
- Column Volume: 1 ml
- Columns Dimension: 0.7 x 2.5 cm
- Column Harware Pressure Limit: 0.5 MPa
- Column Hardware: Polypropylene (Biocompatible)
G-Trap™ rProtein A, 5 ml columns
- Column Volume: 5 ml
- Columns Dimension: 1.6 x 2.5 cm
- Column Harware Pressure Limit: 0.5 MPa
- Column Hardware: Polypropylene (Biocompatible)
Application (s):
- G-Trap™ rProtein A FF are prepacked ready to use columns used for affinity purification of IgG antibodies.