The SPINeasy Host Depletion Microbial DNA Kit provides an easy-to-use workflow to isolate and enrich microbial DNA from samples containing high amounts of host DNA. Host DNA contamination is common in samples such as swabs and bodily fluids. The presence of high amounts of human DNA can limit the application and sensitivity of downstream shotgun metagenomic sequencing or microbiome analysis, as most of the extracted DNA is from human and not microbial origin.
The SPINeasy Host Depletion Microbial DNA Kit provides efficient reduction of host DNA from these samples by selective lysis of host cells with our specially formulated Host Lysis Buffer. Host DNA, which has been released into the solution, is then removed from the supernatant through centrifugation. A further host depletion treatment provides enzymatic degradation of any remaining host DNA, as well as DNA coming from dead microbial cells. Finally, the remaining intact microbial cells are lysed through a combination of specially formulated buffers and highly efficient homogenization using FastPrep instruments from MP Biomedicals. Enriched microbial DNA is then purified using a convenient silica membrane spin-column technology workflow and ready for downstream molecular applications, such as PCR and NGS.
SPINeasy Host Depletion Microbial DNA Kit is designed to overcome the challenges that researchers face when working with heavily contaminated host DNA samples.
Microbiome analysis, Shot-gun metagenomics sequencing, NGS, Genomics, Molecular Biology
Key features and details
- Provides an easy-to-use workflow to isolate microbial DNA from samples containing high amounts of host DNA
- Selective lysis of host cells and depletion followed by microbial DNA purification
- Lysing Matrix E for thorough and complete microbial lysis with FastPrep instruments
- For enrichment and analysis of microbial DNA from swabs and bodily fluid samples
Alternate Names: SPINeasy Host Depletion Microbial DNA Kit; Host DNA Depletion Kit
Application Notes: SPINeasy Host Depletion Microbial DNA Kit is designed to overcome the challenges that researchers face when working with heavily contaminated host DNA samples.
Base Catalog Number: 65450
Components: 12 mL Equilibration buffer,50x Lysing Matrix E, 60 mL Host Lysis Buffer, 6 mL Host Depletion Buffer, 1 Vial Host Depletion Enzyme HDE, 200 µL HDE Reconstitution Buffer, 12 mL Microbial Selection Buffer, 40 mL Microbial Lysis Buffer, 30 mL Wash Buffer HD1, 6 mL Wash Buffer HD2, 6 mL Wash Buffer HD, 50x Column HD with collection tubes
Pack Size: 50 Preps
Sample Type: Saliva, Swabs, Bodily Fluid
Storage: Store at Room Temperature (15-25?) in a dry environment