SPINeasy DNA Kit for Microbiome allows for efficient and unbiased lysis of microbes, including gram positive/negative bacteria, fungi, protozoans, and viruses found in various swab, body fluid, stool, soil, and water samples. This kit features a highly effective lysis system and silica membrane spin column technology. With the use of specially formulated Buffer MB1 and Lysing Matrix E in combination with FastPrep instruments from MP Biomedicals, efficient lysis of various samples can be achieved within seconds. Provided with the kit, Column MB and kit buffers are designed to deliver gDNA of high yield and purity in fewer steps, ready for downstream applications such as qPCR, restriction digestion, and sequencing.
Application Notes:
Efficient and unbiased lysis of microbes, including gram positive/negative bacteria, fungi, protozoans, and viruses found in swab, sputum, body fluid, stool, soil, and water samples.
SPINeasy® DNA Kit for Microbiome employs a novel method for isolating microbial genomic DNA from various samples. Due to its highly effective lysis capability and silica membrane spin column technology, the SPINeasy DNA Kit for Microbiome can be used for bacterial, body fluids, and environmental samples such as soil and stool.
Usage Statement:
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use.
Key Applications:
Genomic, Molecular Biology, PCR, qPCR, Microbiome analysis, NGS, Shotgun sequencing
Key features and details
- Provides an easy-to-use workflow to isolate microbial DNA from samples containing high amounts of host DNA
- Selective lysis of host cells and depletion followed by microbial DNA purification
- Lysing Matrix E for thorough and complete microbial lysis with FastPrep instruments
- For enrichment and analysis of microbial DNA from swabs and bodily fluid samples
Alternate Names: SPINeasy Host Depletion Microbial DNA Kit; Host DNA Depletion Kit
Application Notes: SPINeasy Host Depletion Microbial DNA Kit is designed to overcome the challenges that researchers face when working with heavily contaminated host DNA samples.
Base Catalog Number: 65450
Brand: SPINeasy
Components: 12 mL Equilibration buffer,50x Lysing Matrix E, 60 mL Host Lysis Buffer, 6 mL Host Depletion Buffer, 1 Vial Host Depletion Enzyme HDE, 200 µL HDE Reconstitution Buffer, 12 mL Microbial Selection Buffer, 40 mL Microbial Lysis Buffer, 30 mL Wash Buffer HD1, 6 mL Wash Buffer HD2, 6 mL Wash Buffer HD, 50x Column HD with collection tubes
Pack Size: 50 Preps
Sample Type: Saliva, Swabs, Bodily Fluid
Storage: Store at Room Temperature (15-25?) in a dry environment