Venor®GeM OneStep is a complete kit which includes all reagents required for PCR. Primer, nucleotides, polymerase and the internal amplification control are provided ready-to-use in a lyophilized reaction mix. The included rehydration buffer is added to the mix, aliquots made according to the sample number, sample or Positive Control DNA added and the setup is ready for PCR.
Type of PCR
For conventional, endpoint PCR
Scope of Application
Applicable in research and industry for direct testing of cell cultures and biologicals. Intended for research use only. Not recommended for clinical diagnostics or testing of human samples.
Kit Components
- Primer sets, nucleotides, internal amplification control and polymerase at optimized concentrations lyophilized in one tube
- Rehydration buffer
- Lyophilized positive control
- Tris/EDTA buffer
Package Sizes
Primer sets and nucleotides are prepared in aliquots of 25 tests.
Result evaluation
Gel electrophoresis at endpoint of PCR
Required Consumables
- Agarose gels and DNA stains
- PCR reaction tubes
Required lab devices
- Gel electrophoresis and documentation system / Regular PCR cycler
- Pipetting equipment
- Tube centrifuge
Shelf Life and Storage
Venor®GeM OneStep components are maintainable at +2 to +8 °C for at least 6 months. After rehydratisation the reagents must be stored at -18 °C.
EP 2.6.7 compliance
No