REDExtract-N-Amp(TM) Tissue PCR Kit,

Storage: -20C

EC Number: 254-457-8

UNSPSC Code: 12352200

Features and Benefits: . Perfect for quick genomic DNA isolation for genotyping. Single-step extraction of genomic DNA for PCR in 15 minutes. PCR ReadyMix, specially formulated for amplification directly from extract. Hot Start antibody for highly specific PCR amplification of genomic DNA. Extract stable at 4 oC for at least 6 months

Application: REDExtract-N-Amp(TM) tissue PCR kit has been used for DNA extraction and in polymerase chain reaction (PCR).

Components: Kits contain Tissue Preparation Solution, Extraction Solution, and REDExtract-N-Amp PCR Reaction Mix.

Principle: The kit comes with validated protocols to extract and amplify genomic DNA from mouse-tails, hair, animal tissue, saliva, and buccal swabs. In a typical procedure, genomic DNA is extracted from a sample that has been incubated in the tissue preparation solution and extraction solution for 10 minutes at room temperature. The sample is heated to 95 C for 3 minutes and then mixed with a third solution to neutralize inhibitory substances prior to PCR. A portion of the DNA extract is then added to a PCR reaction containing primers and REDExtract-N-Amp PCR ReadyMix, included in the kit.

Other Notes: For additional information, please see www.sigma-aldrich.com/extract-n-amp.

Other Notes: The REDExtract-N-Amp Tissue PCR Kits provide all the reagents necessary to rapidly extract DNA from a wide variety of cells and tissues and amplify targets of interest by PCR. A novel extraction method eliminates the need for long enzymatic digestions or homogenization. The kit also includes a specially formulated hot start PCR reaction mix for amplification directly from the extract. The REDExtract-N-Amp PCR ReadyMix contains an inert dye that acts as a tracking dye and allows for convenient loading of PCR reactions onto agarose gels for analysis.