• PRODUCT AVAILABILITY: Did you know you can view a product's availability right on the product page? Simply enter the quantity you want to purchase and the current availability will appear below the item.

  • Product is restricted and can only be purchased by customers with a web profile linked to a Thomas Business Account - Click here to login or create your web profile. If you do not have a Thomas Business Account, please Contact Us and someone will respond with details on how to apply for a business account with us.

DNA-pure Yeast Genomic Kit

Not yet rated

The DNA-Pure Yeast Genomic Kit provides a rapid, 30 minute protocol for the isolation of high quality, intact yeast genomic DNA. This procedure eliminates the need for enzyme digestions and organic extractions which can result in sheared, variable quality DNA. The DNA obtained using the DNA-Pure Yeast Genomic Kit provides for excellent restriction digestion and PCR results.

Typical yields from this kit are 10 - 20 ug DNA from a 1.5-3 ml culture. The kit has been tested successfully with Saccharomyces cervisiae, Schizosaccharomyces pombe, and Pichia pastoris. The Kit utilizes an easy to follow protocol: 1) pellet cells; 2) resuspend cells and add lysis solution; 3) add protein solubilization solution after a 10 minute incubation at 70 C; 4) centrifuge and then isopropanol precipitate DNA from the supernatant; 5) wash pellet and resuspend in TE or water.

Advantages:Easy 30 minute protocol High yield of quality, intact yeast genomic DNA. No lytic enzymes or proteinase K needed. No organic extractions. DNA can be used directly for restriction enzyme digestion, PCR, and hybridization.

Components:
Resuspension Buffer I, Lysis Buffer, Protein Solubilization Buffer, Resuspension Buffer II.

Please Enter Your Order Info

Filter by:

  • Clear Filters
Product Detail
Thomas No.
C995W46
Mfr. No.
10760006-1
UN #
41105504
Description
DNA-Pure Yeast Genomic Kit, 50 Prep(s)
Packaging Size
50 units
list price/quantitytotal
$0.00
Thomas No.
C995W47
Mfr. No.
10760006-2
UN #
41105504
Description
DNA-Pure Yeast Genomic Kit, 100 Prep(s)
Packaging Size
100 units
list price/quantitytotal
$0.00
$0.00 (0 Items)
Product is restricted and can only be purchased by customers with a web profile linked to a Thomas Business Account - Click here to login or create your web profile. If you do not have a Thomas Business Account, please Contact Us and someone will respond with details on how to apply for a business account with us.

Write A Review