HiEncap™ Tartoff-Hobbs (HiEncap™ Terrific) Broth is recommended for the cultivation of recombinant strains of Escherichia coli. Suspend 1 capsule in 250 ml (4 capsules in 1000 ml) distilled or purified water containing 0.4% v/v glycerol (i.e 1 ml in 250ml or 4 ml in 1000ml media ).