ORCHID medium is free of serum, animal components and hydrolysates. The medium is developed for maximal living virus and recombinant protein production in suspension cells. If combined with ORCHID Feeds medium supports a viable cell concentration up to 4x107/mL HEK293 suspension cells have been carried for more than 2 years in ORCHID medium with no loss of viability.
ORCHID medium is easy-to-use and suitable for suspension cultures. It requires only minimal or no adaptation from other serum-free media.
Developed to maximize HEK293 cells for living virus and recombinant protein production. The medium provides equivalent of higher growth of HEK293 cells when compared to serum-containing medium. The medium offers
Simplicity: Ideal from bench to manufacturing scale.
Serum-free and animal component free: Reduces the risk for animal derived contaminants.
Fully defined: It doesn’t contain hydrolysates or other complex additives. Thus, process variability is reduced.
Antibiotic-free: No risk of antibiotic traces, hidden contamination and endotoxins.
ORCHID medium is dedicated to maximal production of wide range of living viruses, e.g.adeno virus, lenti virus, influenza virus.ORCHID is also suitable for recombinant protein expression for human like glycosylation, for example Erythropoietin, antibodies, Fc-fusion proteins. ORCHID is not suitable for transient expression or viral vector production. For this application we have developed the special medium IVY.
Regulatory: Serum-free, animal component free, hydrolysate free,
Available Form: Liquid & Powder
Cell Line: HEK293 suspension cells
Storage Condition: 2-8 oC, protect from light
Application: For research and manufacturing