Endothelial Cell Growth Supplement (ECGS) is a broadly used supplement to culture a variety of cells, particularly endothelial cells. ECGS contains various growth factors (e.g., acidic FGF or ECGF-a).
Recommended concentration - 50-150 µg/ml of medium depending on cell type. Some primary cells require supplementation of 1-100 µg/ml heparin.
- Tested for ability to promote proliferation of Fetal Bovine Heart Endothelial Cells (FBHEC) in medium containing 10% newborn calf serum
- Filtered (0.2 µm membrane) and tested and found negative for bacteria, fungi and mycoplasma
Stable for at least three months at 2° to 8°C (lyophilized) or = one month at -20°C (solubilized). Avoid multiple freeze thaws.
Lyophilized from NaCl-containing buffer with streptomycin sulfate; reconstitute in serum-free medium.