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  • Fuchsin Lactose Broth

    HiMedia
    It has been agreed by most workers in water bacteriology that the plain Lactose Broth usually used for presumptive test is not altogether satisfactory, in that it gives many false positive tests. A number of modifications have been suggested to eliminate as far as possible, these false positive tests…
  • Lactose Broth

    HiMedia
    Lactose Broth is recommended by APHA in the performance and confirmation of the presumptive test for coliform bacteria in water (1), food (2) and milk (3). Present formulation is recommended by BIS for detection and estimation of coliform bacteria in food stuff as a confirmatory medium (4). As per BIS…
  • Lactose Broth

    HiMedia
    …waters or drinking water (1). Lactose Broth is recommended by APHA in the performance and confirmation of the presumptive test for coliform bacteria in water (2), food (3) and milk (4). This medium was initially listed as an alternative to Lauryl Sulfate Broth in the presumptive Standard Total Coliform…
  • Eijkman Lactose Broth

    HiMedia
    Coliform organism is a term used to designate the lactose-fermenting Enterobacteria such as Escherichia coli and Enterobacter . Enterobacteriaceae forms a large group of gram-negative bacteria that inhabit intestinal tract of warmblooded animals. Therefore they constitute the major microbial flora…
  • M-Brilliant Green Broth

    HiMedia
    …Clark (2) applied M-Brilliant Green Broth for primary screening of Salmonella in polluted water. This selective differential medium is a modification of Brilliant Green Agar without agar in double strength (3). Salmonella are unable to ferment either lactose or saccharose in the medium. This allows…
  • M-EMB Broth

    HiMedia
    lactose (1). M-EMB Broth is a selective differential medium used for the detection of members of the coliform group by membrane filtration. M-EMB Broth is formulated as per Clark et al (2). Proteose peptone and yeast extract serve as sources of carbonaceous and nitrogenous growth nutrients. Lactose
  • MacConkey Broth with Neutral Red

    HiMedia
    MacConkey Broth is the earliest selective and differential medium for cultivation of enteric microorganisms from a variety of clinical specimens (4,5). Subsequently MacConkey Broth have been recommended for use in microbiological examination of foodstuffs (6) and for direct inoculation of water samples…
  • MacConkey Broth (MacC)

    HiMedia
    …medium falls below 6.8. Lactose non-fermenting organisms like Salmonella and Shigella do not alter the appearance of the medium. Transfer homogenate in Soybean Casein Digest Medium (MU011) containing 1 gm or 1 ml of the preparation to be examined to 100 ml MacConkey Broth Incubation is carried at…
  • MacConkey Broth Purple with BCP

    HiMedia
    …MacConkey Broth Purple w/ BCP is recommended by ISO committee (3) with the inclusion of bile salts, as a presumptive test medium for identification of coliforms from water and other materials of sanitary importance. Peptic digest of animal tissue provides essential growth nutrients. Lactose is the…
  • Selenite Broth (Selenite F Broth)

    HiMedia
    …results in overgrowth of other bacteria. The acid produced by bacteria due to lactose fermentation serves to maintain a neutral pH. Sodium phosphate maintains a stable pH and also lessens the toxicity of selenite. Enriched broth is subcultured on differential plating media such as Bismuth Sulphite Agar…
  • MacConkey Broth Purple with BCP

    HiMedia
    MacConkey Broth Purple is recommended for detection and estimation of coliforms in foodstuff (1) and for presumptive identification of coliforms from a variety of specimens such as water, milk and food. This medium is recommended for use in microbiological examination of foodstuff (2) and for direct…
  • MacConkey Broth Purple with BCP

    HiMedia
    MacConkey Broth Purple w/ BCP is a modification of MacConkey Medium (1). Childs and Allen (2) demonstrated the inhibitory effect of neutral red and therefore substituted it by the less inhibitory bromocresol purple dye. BCP is more sensitive in recording pH variation in the medium. Peptic digest of…
  • MUG EC Broth

    HiMedia
    …specific indicator of faecal contamination and the possible presence of enteric pathogens. EC Broth was devised by Hajna and Perry (1) and further modified by addition of the fluorogenic compound MUG. MUG EC Broth is also recommended by APHA for the analysis of drinking water, surface and ground water and…
  • Coliform PA Broth

    HiMedia
    …Coliform P-A broth is adaptable for screening of sample for the presence of alternative indicator organisms (6). Beef extract, pancreatic digest of gelatin, and casein enzymic hydrolysate provides nitrogenous and carbonaceous compounds, vitamin B complex and trace ingredients. Lactose is the fermentable…
  • MUG Brilliant Green Bile Broth

    HiMedia
    …source. During examination of water samples, growth from presumptive positive tubes showing gas in Lactose Broth (M026) or Lauryl Tryptose Broth (M080) is inoculated in Brilliant Green Bile Broth 2% (M121). Gas formation within 48 ? 2 hours confirms the presumptive test (1). Storage and Shelf…
  • M-Lauryl Sulphate Broth

    HiMedia
    …44°C. Subculture to Lactose Broth (M1003) and Tryptone water (M463I) to confirm gas and indole production respectively after 24 hours incubation at 44°C. Coliform organisms which give yellow colonies on membranes incubated at 35-37°C should be subcultured in Lactose Broth (M1003). This can be incubated…
  • Fluid Selenite Cystine Medium (Selenite Cystine Broth)

    HiMedia
    …bacteria due to lactose fermentation serves to maintain a neutral pH. Phosphate maintains a stable pH and also lessens the toxicity of selenite. L-cystine is the reducing agent, improving the recovery of Salmonella . Enriched broth is subcultured on solid medium. Do not incubate the broth longer than 24…
  • Lauryl Sulphate Broth (Lauryl Tryptose Broth)

    HiMedia
    …presence of bile salts and produce acid and gas from lactose within 48 hours at 37°C (1). These bacteria can also be defined as, members of Enterobacteriaceae capable of growing at 37°C, that normally possess b-galactosidase (2). Lauryl Sulphate Broth is used for the detection of coliforms in water,…
  • Selenite Cystine Broth Base

    HiMedia
    …other bacteria. The acid produced by bacteria due to lactose fermentation serves to maintain a neutral pH. Sodium phosphate maintains a stable pH and also lessens the toxicity of selenite. L-cystine improves recovery of Salmonella . Enriched broth is subcultured on differential plating media such as…
  • Taurocholate Broth

    HiMedia
    Taurocholate broth selectively supports growth of gram-negative coliforms from water, milk and other similar food products. The selective action of this medium is attributed to crystal violet and sodium taurocholate which inhibits most species of grampositive bacteria. Lactose is a fermentable carbohydrate…

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