Fulfills the needs of reproducibility, specificity and speed which are requirements for PCR in any application
• Robust aluminum block
• Versatile temperature gradient over 12 rows
• Gradient range of up to 20°C
• vapo.protect™ technology
The proprietary vapo.protect technology reduces evaporation to a minimum. Therefore, concentrations in your PCR master mix remain consistent leading to stable and reproducible specificity. Non-specific binding is minimized beyond importance.
The extremely high heating and cooling rates of the Mastercycler pro S give you the speed you need.
Unsurpassed speed can be achieved with Eppendorf’s Impulse PCR, a device driven hot-start function that increases heating rates to 8°C/s.
Evaporation in a PCR reaction vessel not only limits the ability to work with low reaction volumes but also makes it difficult to obtain reproducible results. For the Mastercycler pro it has been Eppendorf’s aim to protect the PCR reaction from evaporation. While common PCR instruments press a metal plate onto the PCR consumable, the vapo.protect lid covers the PCR consumable with a cushion that conforms to the shape of the consumable - no matter which consumable you use. The snug fit of the cushion in combination with the high pressure and excellent heating capabilities of the lid reduce evaporation to a minimum.
Evaporation leads to increasing concentrations of the PCR master mix components, especially primers. This, in turn, leads to non-specific binding. The changes in concentrations due to evaporation both during the experiment and between experiments can be a cause for great concern for any scientist who tries to keep variables to a minimum. The flexible fit of the fluid cushion minimizes evaporation of your reaction considerably. Only 0-3% of the reaction volume evaporates using the new vapo.protect technology.
The gradient option of the Mastercycler pro features the SteadySlope® technology to ensure that the heating and cooling ramp rates are identical in both gradient and normal operation. Thus, you can expect identical temperature control characteristics in both optimization and routine experiments. Programming the gradient with the Control Panel and the CycleManager pro is easy and intuitive - so that novice users can get started quickly and safely.
The self test function of the Mastercycler pro provides the ability to check the peltier elements of the thermal block easily. This feature tests the functionality of all six peltier elements and provides a corresponding certificate. This way, the user can document the homogeneity of the instrument between regular testing intervals. In addition, there is a Temperature Validation System (1228K31) available as an external testing device.
