• Promotes the rapid differentiation of Caco-2 cells in vitro - saving both time and labor
• Permits testing for intestinal permeability, including bioavailability, with Caco-2 cells in three days instead of three weeks
• Time Saving
• Labor Saving and Increased Productivity
• Maximum Convenience
• Automation Compatible
• Greater Reliability and Uniform Assay Conditions
By combining the benefits of the BD BioCoat Intestinal Epithelial Environment with the automation-friendly BD Falcon™ 24-Multiwell Insert System, the BD BioCoat HTS Caco-2 Assay System is an integrated, robotics-compatible cell environment designed to promote the rapid differentiation of Caco-2 cells in vitro. The specialized media included with the BD BioCoat HTS Caco-2 Assay System have been formulated to produce differentiated monolayers in just three days. Each system contains a fibrillar collagen-coated 24-Multiwell Insert Plate suitable for robotic screening of prospective pharmaceuticals for oral bioavailability and absorption.
Unique fibrillar collagen-coated inserts used with our specialized media promotes rapid differentiation of Caco-2 cells in three days. The accelerated three-day protocol along with an automation-friendly format saves considerable labor over traditional 21-day differentiation protocols. The large volume Feeder Tray reduces the need for frequent media changes. Each system includes a custom-designed Feeder Tray and automation-friendly lid. In use, the unique media Feeder Tray enhances well-to-well uniformity by bathing all 24 inserts in the same medium. The system is designed for use with most robotics that accept BD Falcon 24-well plates. The system may also be used manually with individual or multichannel pipetters. The entire system is tested for lot-to-lot consistency and qualified for use in Caco-2 screening assays. Therefore, this system can be used with complete confidence.
An in vitro system optimized for rapid differentiation of Caco-2 cells in an automation-friendly format.
Quality Control:
Tested for ability to promote formation of a differentiated monolayer of Caco-2 cells with barrier function within 72 hours of cell seeding (measured by TEER and mannitol permeability). BD Intestinal Epithelium Seeding and Differentiation Media, and BD Mito+ Serum Extender tested for myoplasma. BD Intestinal Epithelium Differentiation Medium tested for endotoxin (LAL assay). All components tested and found negative for the presence of bacteria and fungi.
Storage and Stability:
Store at 2-8°C, away from direct sunlight, do not freeze.; Shelf life: At least 3 months from date of shipment. Upon supplementation with BD Mito+ Serum Extender, both the BD Intestinal Epithelium Seeding and Differentiation Media are stable for 21 days under subdued lighting at 2-8°C.
NOTE: Kit contains: BD BioCoat Fibrillar Collagen 24-Multiwell Insert System plus media to perform 24 individual three-day Caco-2 assays
