• Evaluate endothelial cell invasion using real-time fluorescence detection in a simplified and reproducible manner

 • Increase screening throughput for prospective pro- and anti-angiogenic compounds

During angiogenesis, endothelial cells are activated and express matrix metalloproteinases (MMPs), which degrade the vascular basement membrane and facilitate cellular movement towards angiogenic stimuli. The BD BioCoat Angiogenesis System: Endothelial Cell Invasion provides an in vitro, quantitative assay for prospective anti- and pro-angiogenic compounds. It is composed of a BD Falcon™ 24-Multiwell Insert Plate (and a non-TC-treated 24- or 96-well receiver plate and lid) containing a BD FluoroBlok™ fluorescence-blocking microporous (3.0 µm pore size) polyethylene terephthalate (PET) membrane coated with BD Matrigel™ Matrix. BD Matrigel Matrix is a reconstituted basement membrane preparation derived from Engelbreth-Holm-Swarm (EHS) mouse tumors. Furthermore, this matrix is primarily composed of laminin, collagen IV, entactin, and a number of growth factors. An optimized coating process effectively occludes the membrane pores and provides a functional barrier that is analogous to the basement membrane in vivo. This coating blocks the passage of non-invasive cells and allows the passage of activated endothelial cells with invasive capacity. Following activation by angiogenic factors, the endothelial cells express MMP 2 and 9. These proteases digest the matrix and enable the cells to invade through the matrix barrier to the bottom side of the microporous membrane.

Quality Control:
Tested for its ability to allow invasion of HMVEC-1 cells, an invasive human microvascular endothelial cell line, and to exclude invasion of NIH-3T3 cells, a non-invasive fibroblast cell line.
Tested and found negative for bacteria and fungi.

Storage and Stability:
Stable for at least three months from the date of shipping when stored at -20°C.