• Cell culture on permeable membranes permits diffusion of media components to both apical and basolateral cell surfaces

 • Membranes with extracellular matrix (ECM) further improve
in vitro cell culture systems by providing cells with relevant protein substrates

BD BioCoat Cell Culture Inserts contain BD Falcon™ Cell Culture Inserts coated with extracellular matrix (ECM) and are packaged ready-to-use in BD Falcon Companion Plates. Cell culture on microporous membranes has been shown to promote differentiation of a variety of epithelial and mesenchymal cells in vitro, especially in the presence of ECM.
ECM is a vital component of the cellular microenvironment in vivo. The use of ECM growth substrates in vitro has been shown to promote cell growth, cell-specific morphology, and differentiated function. For example, collagen I and fibronectin have been used on microporous inserts to establish barrier function in monolayers of intestinal epithelial and endothelial cells, to culture a renal cell line that expresses kidney-specific peptide transporters, and to culture keratinocytes. Additionally, BD BioCoat Matrigel™ Matrix coated inserts have been used to maintain polarized secretion in uterine, thyroid and mammary epithelial cells.
The track-etched polyethylene teraphthalate (PET) membranes used in BD BioCoat Cell Culture Inserts have individual cylindrical pores in a broad range of sizes including 0.4, 1.0, 3.0, and 8.0 µm.

Applications include:

 • Promotion of epithelial cell polarity

 • Differentiation of a variety of cell types

 • Transport and permeability studies

 • Transendothelial migration

 • Tumor cell invasion assays

 • Chemotaxis and haptotaxis assays

 • In vitro toxicology

 • Co-culture studies

The PET membrane offers several advantages:

 • Low binding properties minimize the loss of small molecules, including low molecular weight proteins and other natural and synthetic compounds. Therefore, the membrane is ideal for transport or intercellular communications studies

 • Low pore-density membranes are transparent, allowing living cells to be viewed with a phase contrast microscope

 • Exceptional durability assures membranes are compatible with most solvents used during fixation and staining protocols for light and electron microscopy

 • Membranes will not tear or curl and remain easy to handle when removed from insert housing

BD BioCoat Cell Culture Inserts offer:

 • BD Falcon Cell Culture Inserts’ innovative hanging design facilitates pipetting and reduces risk of medium wicking

 • BD Falcon Companion Plates’ labyrinth lid design and condensation rings reduce evaporation and risk of contamination

 • Choice of 6-, 12-, and 24-well plate sizes

 • An extensive selection of ECMs for improved cell attachment morphology, and functionality

BD BioCoat Cell Culture Inserts are available with the following selection of ECMs:Three-Dimensional Matrices: BD Matrigel Matrix, Fibrillar CollagenThin-Layer Matrices: Collagen I, Fibronectin, Collagen IV, Laminin

Quality Control:
All cell culture inserts are tested for ability to promote cell attachment and spreading or differentiation (cell type used is specific for each ECM). Tested and found negative for bacteria and fungi.

Storage and Stability:
BD BioCoat Collagen I, Collagen IV, Fibrillar Collagen, Fibronectin, and Laminin Inserts stable for = three months at 2-8°C. BD BioCoat Matrigel™ Matrix Inserts stable for = three months at -20°C. BD BioCoat Control Inserts and Deep-Well Plates stable at room temperature.

BD BioCoat Collagen I Cell Culture Inserts

Thomas P/N# Manufacturer P/N# Short Description Price QTY 1232C94 354442 0.4 µm inserts in four 6-well plates, Cs. 24 $241.19 1232C95 354490 0.4 µm inserts in four 12-well plates, Cs. 24 $223.12 1232C96 354444 0.4 µm inserts in two 24-well plates, Cs. 24 $213.56 1232C97 354580 1.0 µm inserts in four 6-well plates, Cs. 24 $241.19 1232C98 354581 1.0 µm inserts in four 12-well plates, Cs. 24 $233.75 1232C99 354482 1.0 µm inserts in two 24-well plates, Cs. 24 $219.94 1232D00 354540 3.0 µm inserts in four 6-well plates, Cs. 24 $246.50 1232D01 354565 3.0 µm inserts in four 12-well plates, Cs. 24 $223.12 1232D02 354541 3.0 µm inserts in two 24-well plates, Cs. 24 $213.56

BD BioCoat Collagen IV Cell Culture Inserts

Thomas P/N# Manufacturer P/N# Short Description Price QTY 1232D03 354591 1.0 µm inserts in two 24-well plates, Cs. 24 $247.56 1232D04 354544 3.0 µm inserts in four 6-well plates, Cs. 24 $272.00 1232D05 354545 3.0 µm inserts in two 24-well plates, Cs. 24 $242.25

BD BioCoat Fibrillar Collagen Cell Culture Inserts

Thomas P/N# Manufacturer P/N# Short Description Price QTY 1232D06 354472 1.0 µm inserts in four 6-well plates, Cs. 24 $269.87 1232D07 354473 1.0 µm inserts in four 12-well plates, Cs. 24 $261.38 1232D08 354474 1.0 µm inserts in two 24-well plates, Cs. 24 $253.94

BD BioCoat Fibronectin Cell Culture Inserts

Thomas P/N# Manufacturer P/N# Short Description Price QTY 1232D09 354440 0.4 µm inserts in four 6-well plates, Cs. 24 $285.81 1232D10 354445 0.4 µm inserts in two 24-well plates, Cs. 24 $247.56 1232D11 354585 1.0 µm inserts in two 24-well plates, Cs. 24 $244.38 1232D12 354492 3.0 µm inserts in four 12-well plates, Cs. 24 $260.31 1232D13 354543 3.0 µm inserts in two 24-well plates, Cs. 24 $242.25

BD BioCoat FluoroBlok™ Fibronectin Cell Culture Inserts

Thomas P/N# Manufacturer P/N# Short Description Price QTY 1232D14 354597 3.0 µm inserts in two 24-well plates $290.06

BD BioCoat Collagen I 24-Multiwell Insert System

Thomas P/N# Manufacturer P/N# Short Description Price QTY 1232D15 354598 3.0 µm insert plate with 24-well plate and lid $227.37

BD BioCoat Fibronectin 24-Multiwell Insert System

Thomas P/N# Manufacturer P/N# Short Description Price QTY 1232D16 354599 3.0 µm insert plate with 24-well plate and lid $258.19

BD BioCoat Laminin Cell Culture Inserts

Thomas P/N# Manufacturer P/N# Short Description Price QTY 1232D17 354446 0.4 µm inserts in two 24-well plates, Cs. 24 $244.38 1232D18 354547 3.0 µm inserts in two 24-well plates, Cs. 24 $249.69

BD BioCoat Matrigel™ Matrix Cell Culture Inserts

Thomas P/N# Manufacturer P/N# Short Description Price QTY 1232D19 354443 0.4 µm inserts in four 6-well plates, Cs. 24 $328.31 1232D20 354447 0.4 µm inserts in two 24-well plates, Cs. 24 $281.56