• Promotes neuronal cell attachment and differentiation for a variety of applications

BD BioCoat Poly-D-Lysine/Laminin (PDL/LM) and Poly-L-Ornithine/Laminin (PLO/LM) Cellware is suitable for culturing many different types of peripheral and central nervous system (CNS)_networks and is useful for promoting neural cell attachment and differentiation. BD BioCoat Laminin/Fibronectin (LM/HFN) Cellware provides an in vitro environment that promotes cell attachment and extensive process formation. In addition, this substrate upregulates receptor systems that are integral to neuronal communications. BD BioCoat LM/HFN Cellware facilitates the study of pharmacological or physiological events of the CNS by providing molecular cues required to produce neuronal cultures capable of responding to many important neurotransmitters, neuropeptides, and excitatory amino acids such as glutamate, NMDA, and kainate.
This line of tissue culture vessels have a uniform application of PDL/LM, PLO/LM, or LM/HFN. BD BioCoat PDL/LM, PLO/LM, and LM/HFN Cellware are manufactured in a highly controlled environment and rigorously tested to assure product consistency and performance.

Applications include:

 • Enhancement of neuronal cell attachment to plastic and glass

 • Promotion of neurite outgrowth

 • Culture of glial cells as feeder layers for neurons

 • Construction of neural cell model systems to study CNS function development and diseases

Quality Control:
PDL/LM and PLO/LM are tested for ability to promote neurite outgrowth with primary rat cerebellar granule cells (RCG) and NG-108 rat glioma/mouse neuroblastoma cells. Tested and found negative for bacteria and fungi.

Storage and Stability:
Cellware stable for at least three months at 2-8°C. Do not freeze. Coverslips and CultureSlides stable for at least three months from date of shipment when stored at 2-8°C.

PDL/LM Multiwell and Assay Plates

Thomas P/N# Manufacturer P/N# Short Description Price QTY 1232C67 354595 PDL/LM PLATES, 6-Well, Cs. 5 $142.80 1232C68 354619 PDL/LM PLATES, 24-Well, Cs. 5 $146.15 1232C69 354596 PDL/LM PLATES, 96-Well, Cs. 5 $149.51

PLO/LM Multiwell and Assay Plates

Thomas P/N# Manufacturer P/N# Short Description Price QTY 1232C74 354658 PLO/LM PLATES, 6-Well, Cs. 5 $142.80 1232C75 354659 PLO/LM PLATES, 24-Well, Cs. 5 $146.15 1232C76 354657 PLO/LM PLATES, 96-Well, Cs. 5 $149.51

LM/HFN Multiwell and Assay Plates

Thomas P/N# Manufacturer P/N# Short Description Price QTY 1232C52 354670 LM/HFN PLATES, 96-Well, Cs. 5 $158.42

PDL/LM Culture Dishes

Thomas P/N# Manufacturer P/N# Short Description Price QTY 1232C70 354455 PDL/LM CULTURE DISHES, 100 mm, Cs. 10 $168.47

PDL/LM Coverslips

Thomas P/N# Manufacturer P/N# Short Description Price QTY 1232C71 354087 PDL/LM COVERSLIPS, 12 mm, Round, Cs. 80 $166.22

PDL/LM Culture Slides

Thomas P/N# Manufacturer P/N# Short Description Price QTY 1232C72 354687 PDL/LM CULTURE SLIDES, 2-Well, Cs. 12 $253.25 1232C73 354688 PDL/LM CULTURE SLIDES, 8-Well, Cs. 12 $261.06