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• Promotes neuronal cell attachment and differentiation and adhesion of transfected cell lines

Poly-D-Lysine (PDL) are synthetic molecules used to enhance cell attachment to plastic and glass surfaces. For many anchorage-dependent cells, the nature of the culture substrate has a major effect on cell growth and the requirement for serum proteins. Tissue culture plastic has a net negative surface charge, which is produced by plasma treatment of the polystyrene. Over the years, many researchers have shown that serum-free or reduced serum cultures can be dramatically improved by coating the culture surface with positively charged polymers, i.e., PDL. Poly-Lysine surface treatment improves adhesive properties by altering the charge on the vessel surface from negative to positive. In addition to promoting cell adhesion, Poly-Lysine also enhances the adsorption of serum or extracellular matrix proteins to the culture substrate.
BD BioCoat Poly-Lysine Cellware is a line of tissue culture vessels with a uniform application of poly-lysine. This cellware is manufactured in a highly controlled environment and vigorously tested to assure product consistency and performance.

Applications include:

• Attachment and spreading of a variety of cell types

• Cell differentiation and neurite outgrowth

• Attachment of fastidious transfected cell lines

• Support survival of primary neurons in culture

• Serum-free or reduced serum culture-

Quality Control:
Tested for ability to promote firm attachment of RCG cells. Tested and found negative for bacteria and fungi.

Storage and Stability:
Cellware stable for six months from date of shipment at 4-30°C. Coverslips, CultureSlides, and Coverslip-Bottom Dishes stable for at least three months from date of shipment at 4°C.

Multiwell and Assay Plates

Thomas P/N#	Manufacturer P/N#	Short Description	Price	QTY
1232B52	354413	POLY-D-LYSINE PLATES, 6-Well, Cs. 5	$62.48
1232B53	356413	POLY-D-LYSINE PLATES, 6-Well Cs. 50	$368.16
1232B54	354470	POLY-D-LYSINE PLATES, 12-Well, Cs. 5	$65.83
1232B55	356470	POLY-D-LYSINE PLATES, 12-Well, Cs. 50	$373.74
1232B56	354414	POLY-D-LYSINE PLATES, 24-Well, Cs. 5	$65.83
1232B57	356414	POLY-D-LYSINE PLATES, 24-Well, Cs. 50	$373.74
1232B58	354509	POLY-D-LYSINE PLATES, 48-Well, Cs. 5	$88.14
1232B59	356509	POLY-D-LYSINE PLATES, 48-Well, Cs. 50	$518.76
1232B60	354461	POLY-D-LYSINE PLATES, 96-Well, Clear, Cs. 5	$69.18
1232B61	356461	POLY-D-LYSINE PLATES, 96-Well, Clear, Cs. 50	$406.09
1232B62	356690	POLY-D-LYSINE PLATES, 96-Well, Clear, Cs. 80	$640.37
1232B63	354640	POLY-D-LYSINE PLATES, 96-Well, Black/Clear, Cs. 5	$123.84
1232B64	356640	POLY-D-LYSINE PLATES, 96-Well, Black/Clear, Cs. 50	$730.74
1232B65	356692	POLY-D-LYSINE PLATES, 96-Well, Black/Clear, Cs. 80	$1,159.14
1232B66	354651	POLY-D-LYSINE PLATES, 96-Well, White/Clear, Cs. 5	$123.84
1232B67	356651	POLY-D-LYSINE PLATES, 96-Well, White/Clear, Cs. 50	$730.74
1232B68	356693	POLY-D-LYSINE PLATES, 96-Well, White/Clear, Cs. 80	$1,159.14
1232B69	354620	POLY-D-LYSINE PLATES, 96-Well, White, Cs. 5	$78.11
1232B70	356620	POLY-D-LYSINE PLATES, 96-Well, White, Cs. 50	$457.41
1232B71	356691	POLY-D-LYSINE PLATES, 96-Well, White, Cs. 80	$734.08
1232B72	354662	POLY-D-LYSINE PLATES, 384-Well, Clear, Cs. 5	$82.36
1232B73	356662	POLY-D-LYSINE PLATES, 384-Well, Clear, Cs. 50	$567.89
1232B74	356696	POLY-D-LYSINE PLATES, 384-Well, Clear, Cs. 80	$899.52
1232B75	354663	POLY-D-LYSINE PLATES, 384-Well, Black/Clear, Cs. 5	$148.47
1232B76	356663	POLY-D-LYSINE PLATES, 384-Well, Black/Clear, Cs. 50	$1,027.40
1232B77	356697	POLY-D-LYSINE PLATES, 384-Well, Black/Clear, Cs. 80	$1,628.88
1232B78	354660	POLY-D-LYSINE PLATES, 384-Well, White/Clear, Cs. 5	$148.47
1232B79	356660	POLY-D-LYSINE PLATES, 384-Well, White/Clear, Cs. 50	$1,027.40
1232B80	356694	POLY-D-LYSINE PLATES, 384-Well, White/Clear, Cs. 80	$1,628.88
1232B81	354661	POLY-D-LYSINE PLATES, 384-Well, White, Cs. 5	$91.04
1232B82	356661	POLY-D-LYSINE PLATES, 384-Well, White, Cs. 50	$640.50
1232B83	356695	POLY-D-LYSINE PLATES, 384-Well, White, Cs. 80	$1,013.32

Culture Dishes

Thomas P/N#	Manufacturer P/N#	Short Description	Price	QTY
1232B84	354467	POLY-D-LYSINE CULTURE DISHES, 35 mm, Cs. 20	$61.37
1232B85	356467	POLY-D-LYSINE CULTURE DISHES, 35 mm, Cs. 100	$186.32
1232B86	354468	POLY-D-LYSINE CULTURE DISHES, 60 mm, Cs. 20	$64.71
1232B87	356468	POLY-D-LYSINE CULTURE DISHES, 60 mm, Cs. 100	$198.59
1232B88	354469	POLY-D-LYSINE CULTURE DISHES, 100 mm, Cs. 10	$73.63
1232B89	356469	POLY-D-LYSINE CULTURE DISHES, 100 mm, Cs. 40	$175.15
1232B90	354550	POLY-D-LYSINE CULTURE DISHES. 150 mm, Cs. 5	$87.03

Culture Flasks

Thomas P/N#	Manufacturer P/N#	Short Description	Price	QTY
1232B91	354479	POLY-D-LYSINE CULTURE FLASKS, 25 cm2, Plug-Seal, Cs. 10	$95.94
1232B92	356479	POLY-D-LYSINE CULTURE FLASKS, 25 cm2, Plug-Seal, Cs. 50	$285.60
1232B93	354536	POLY-D-LYSINE CULTURE FLASKS, 25 cm2, Vented-Cap, Cs. 10	$101.53
1232B94	356536	POLY-D-LYSINE CULTURE FLASKS, 25 cm2, Vented-Cap, Cs. 50	$298.99
1232B95	354524	POLY-D-LYSINE CULTURE FLASKS, 75 cm2, Plug-Seal, Cs.5	$68.05
1232B96	356524	POLY-D-LYSINE CULTURE FLASKS, 75 cm2, Plug-Seal, Cs. 50	$400.52
1232B97	354537	POLY-D-LYSINE CULTURE FLASKS, 75 cm2, Vented-Cap, Cs. 5	$71.40
1232B98	356537	POLY-D-LYSINE CULTURE FLASKS, 75 cm2, Vented-Cap, Cs. 50	$421.71
1232B99	354495	POLY-D-LYSINE CULTURE FLASKS, 150 cm2, Plug-Seal, Cs. 5	$122.73
1232C00	356495	POLY-D-LYSINE CULTURE FLASKS, 150 cm2, Plug-Seal, Cs. 40	$584.59
1232C01	354538	POLY-D-LYSINE CULTURE FLASKS, 150 cm2, Vented-Cap. Cs. 5	$129.41
1232C02	356538	POLY-D-LYSINE CULTURE FLASKS, 150 cm2, Vented-Cap, Cs. 40	$614.72
1232C03	354529	POLY-D-LYSINE CULTURE FLASKS, 175 cm2, Plug-Seal, Cs. 5	$141.69
1232C04	356529	POLY-D-LYSINE CULTURE FLASKS, 175 cm2, Plug-Seal, Cs. 40	$682.76
1232C05	354539	POLY-D-LYSINE CULTURE FLASKS, 175 cm2, Vented-Cap, Cs. 5	$148.37
1232C06	356539	POLY-D-LYSINE CULTURE FLASKS, 175 cm2, Vented-Cap, Cs. 40	$716.24

Coverslips

Thomas P/N#	Manufacturer P/N#	Short Description	Price	QTY
1232C07	354086	COVERSLIPS, 12 mm, Round, Cs. 80	$155.08

Coverslip-Bottom Dishes

Thomas P/N#	Manufacturer P/N#	Short Description	Price	QTY
1232C08	354077	COVERSLIP-BOTTOM DISHES, 35 mm, Cs. 20	$150.62

Culture Slides

Thomas P/N#	Manufacturer P/N#	Short Description	Price	QTY
1232C09	354566	POLY-D-LYSINE CULTURE SLIDES, 1-Well, Cs. 12	$200.81
1232C10	354629	POLY-D-LYSINE CULTURE SLIDES, 2-Well, Cs. 12	$204.17
1232C11	354577	POLY-D-LYSINE CULTURE SLIDES, 4-Well, Cs. 12	$208.63
1232C12	354632	POLY-D-LYSINE CULTURE SLIDES, 8-Well, Cs. 12	$209.74

(Additional discounts may apply and will appear when viewing cart.)