• Promotes neuronal cell attachment and differentiation and adhesion of transfected cell lines
Poly-L-Lysine (PLL) are synthetic molecules used to enhance cell attachment to plastic and glass surfaces. For many anchorage-dependent cells, the nature of the culture substrate has a major effect on cell growth and the requirement for serum proteins. Tissue culture plastic has a net negative surface charge, which is produced by plasma treatment of the polystyrene. Over the years, many researchers have shown that serum-free or reduced serum cultures can be dramatically improved by coating the culture surface with positively charged polymers, i.e., PLL. Poly-Lysine surface treatment improves adhesive properties by altering the charge on the vessel surface from negative to positive. In addition to promoting cell adhesion, Poly-Lysine also enhances the adsorption of serum or extracellular matrix proteins to the culture substrate.
BD BioCoat Poly-Lysine Cellware is a line of tissue culture vessels with a uniform application of poly-lysine. This cellware is manufactured in a highly controlled environment and vigorously tested to assure product consistency and performance.
Applications include:
• Attachment and spreading of a variety of cell types
• Cell differentiation and neurite outgrowth
• Attachment of fastidious transfected cell lines
• Support survival of primary neurons in culture
• Serum-free or reduced serum culture
Quality Control:
Tested for ability to promote firm attachment of RCG cells. Tested and found negative for bacteria and fungi.
Storage and Stability:
Cellware stable for six months from date of shipment at 4-30°C. Coverslips, CultureSlides, and Coverslip-Bottom Dishes stable for at least three months from date of shipment at 4°C.
Multiwell and Assay Plates
Culture Dishes
Coverslips
