• Highest quantum yield of blue variants

Mutations present in sgBFP™:

 • F64L: improved solubility, and protein folding at 37°C; more rapid and efficient fluorophore formation

 • Y66H; a single excitation peak at 387 nm and emission at 450 nm

 • V163A; improved solubility and protein folding

sgBFP™ SuperGlo™ Blue Fluorescent Protein is a partner for sgGFP. The Y66H mutation generates the spectral shift to a blue emitting variant. The unique combination of the F64L, Y66H and V163A mutations present in sgBFP leads to a QY (Quantum Yield) that approaches the QY of the brightest green variants and is 2-3 fold higher than the QY of mutants without S65T. sgBFP is also more resistant to photobleaching than other BFPs and its spectral properties make it easy to distinguish from GFP or YFP. sgBFP can be readily detected in fluorimeters, FACs and by fluorescent microscopes. Furthermore, the large overlap between the emission peak of sgBFP and the excitation peak of sgGFP make them suitable partners for FRET.