Western Blotting & ELISA

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Western Blotting & ELISA

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  • ELISABright™ Chemiluminescent Substrate

    Advansta

    Chemiluminescent substrate for ELISA applications ELISABright is an enhanced and highly sensitive chemiluminescent HRP substrate optimized for chemiluminescent ELISA applications. This luminol-based substrate demonstrates a wide linear dynamic range and a superior signal to noise ratio.…

  • Grade GB005 Blotting Papers

    GE Healthcare

    1.5 mm highly absorbent paper recommended for semi-dry protein blotting Pure cellulose produced entirely from the highest quality cotton linters with no additives of any kind. Ensures that no contamination will occur during the transfer steps. Manufactured and tested specifically for…

  • Grade GB003 Blotting Papers

    GE Healthcare

    0.8 mm general purpose blotting paper recommended for the lysis/denaturation of colony or plaque lifts as well as Western blots. Pure cellulose produced entirely from the highest quality cotton linters with no additives of any kind. Ensures that no contamination will occur during the…

  • LucentBlue™ X-Ray Film

    Advansta

    High sensitivity X-ray film for chemiluminescent Western blots LucentBlue is a sensitive film ideal for imaging chemiluminescent Western blots. LucentBlue is guaranteed to perform with WesternBright™ ECL, WesternBright Quantum and WesternBright Sirius. LucentBlue is compatible with…

  • PBS With BSA Powder Blend

    Amresco

    Grade: Proteomics

  • WesternBright™ Transfer Membranes

    Advansta

    Convenient Nitrocellulose and PVDF membranes for Western blotting Advansta offers a large selection of nitrocellulose and polyvinylidene difluoride (PVDF) transfer membranes for immunoblotting applications. Choose from convenient pre-cut membranes sized for protein minigels and economical…

  • WesternBright™ ChemiPen™

    Advansta

    Transform your protein standard into a chemiluminescent marker Write or draw on your transfer membranes with the WesternBright ChemiPen. With the proprietary "ink" you can make pre-stained protein standards chemiluminescent, annotate your blot with a date or blot number, or check…

  • Protein Sample Loading Buffers

    Advansta

    Pre-mixed, standard Laemmli loading buffers for protein electrophoresis These ready-to-use Laemmli loading buffers contain Bromophenol blue to enable visualization of the dye front and monitoring of the progress of electrophoresis. Choose from reducing or non-reducing buffers, each supplied…

  • Blot Development Folders

    Advansta

    Transparent plastic supports for blot handling and imaging Advansta's Blot Development Folders are transparent plastic folders that hold your blot while it is exposed to film or a CCD camera. These folders keep your blot flat for perfect film exposures, avoiding the wrinkling or bunching…

  • WesternBright™ ECL HRP Substrate

    Advansta

    The best choice for routine, film-imaged Western blots WesternBright ECL is more sensitive than other chemiluminescent substrates, so you can conduct shorter exposures, load less sample, or use up to ten-times less antibody. Save time and money with WesternBright ECL. Features …

  • WesternOne™ Marker Pen

    ACTGene, Inc.

    HRP Pen for Western Blot Chemiluminescent Detection Add Magic Touch to your Western Blot and Transform Membrane in Seconds Perfect tool for chemiluminescent detection in Western blot. It can be easily used to write/draw on your transfer membranes and make pre-stained marker…

  • Blocking Solution

    Quality Biological

    Blocking is a very important step of western blotting, as it prevents antibodies from binding to the membrane nonspecifically. Blocking is often made with 5% BSA or nonfat dried milk to reduce the background. Nonfat dried milk is often preferred as it is inexpensive and widely available. However,…

  • 1-Step™ Ultra TMB-ELISA Substrate Solution

    Thermo Scientific Pierce

    Thermo Scientific Pierce 1-Step Ultra TMB ELISA Substrate detects horseradish peroxidase (HRP) activity, yielding a blue color (Amax = 370nm and 652nm) that changes to yellow (Amax = 450nm) upon addition of a sulfuric or phosphoric acid stop solution. 1-Step Ultra TMB is the most sensitive of the…

  • WesternBright™ ECL Spray

    Advansta

    The best choice for routine, film-imaged Western blots WesternBright ECL is more sensitive than other chemiluminescent substrates, so you can conduct shorter exposures, load less sample, or use up to ten-times less antibody. WesternBright ECL Spray provides both components of WesternBright…

  • Blotting Papers

    Advansta

    Advansta's pre-cut Blotting Papers are ideal for use in transfer sandwiches and cassettes when proteins are transferred electrophoretically from a gel to a nitrocellulose, PVDF, or other membrane. The Advansta Blotting Papers are available in two sizes to work with multiple transfer setups and…

  • AdvanStain™ Scarlet™ Protein Stain

    Advansta

    AdvanStain Scarlet is a fluorescent stain for gels and blots that allows sensitive and quantitative visualization of proteins. Gels and blots stained with AdvanStain Scarlet can be imaged on any fluorescence imaging system, including laser- and CCD-based systems, with a detection limit of less than…

  • Protein Stain Kit for Transfer Membrane

    Bio Basic Inc.

  • WesternBright™ Sirius™ HRP Substrate

    Advansta

    See bands you couldn't detect before WesternBright Sirius is the most sensitive chemiluminescent substrate available for Western blotting. Its extremely strong signal allows detection of bands unseen with other substrates. Features The most sensitive of Advansta's HRP…

  • Background Quenching Sheets

    Advansta

    For improved imaging of chemiluminescent blots and fluorescent blots and gels Use Advansta's Background Quenching Sheets to obtain the best images of chemiluminescent Western blots and fluorescent blots and gels. For Chemiluminescence Placed under chemiluminescent blots…

  • Rapid Western Blotting Kit-Mouse-WT

    Amresco

    Wet transfer Enables Western Blotting preparation and development in less than 90 minutes. The Rapid Western Blotting Kit provides reagents for all blotting steps from gel transfer, blocking and a combined 1° and 2° antibody incubation step. Users provide the primary antibody and chemiluminescent substrates.

  • Totalblot + PVDF Membranes

    Amresco

    TotalBLOT+™ PVDF Membranes are naturally hydrophobic polyvinylidene fluoride membranes that are well suited for Western transfers, protein binding assays and membrane bound protein sequencing. Our PVDF membranes, pure white microporous solid phase support, exhibit strong hydrophobic…

  • Rapid Western Blotting Kits

    Amresco

    Transfer to development in less than 90 minutes Rapid Transfer Buffer transfers proteins from gel to membrane in 10 - 20 minutes RapidBlock™ efficiently blocks the membrane in 5 minutes Rapid Blot Secondary HRP Antibody is conincubated with primary antibody to save time Use Rapid…

  • AdvanBlock™-PF Blocking Solution

    Advansta

    Non-protein blocking solution for fluorescent and chemiluminescent Western blotting AdvanBlock-PF is a protein-free blocking solution, optimized for use with the WesternBright MCF fluorescent Western blotting kit and also an excellent blocking reagent for chemiluminescent Western blotting.…

  • WesternBright™ Quantum™ HRP Substrate

    Advansta

    Quantitative chemiluminescent Western blotting WesternBright Quantum is formulated to take full advantage of the linear dynamic range of CCD imagers. It provides high sensitivity to detect low abundance proteins, and does not exhibit substrate depletion with high abundance proteins, so has a…

  • AdvanWash™ Washing Solution

    Advansta

    Washing solutions optimized for use with WesternBright systems AdvanWash washing solution is optimized to work with Advansta Western blotting system. Choose AdvanWash for all WesternBright chemiluminescent HRP substrates and the WesternBright MCF fluorescent Western blotting kit.…

  • Sensitive DAB Stain Kit

    Bio Basic Inc.

    DAB, namely,3,3’-diaminobenzidine, as a chromogenic substrate of Peroxidase will lose electron, produce a gradually color change and form insouble dark blown matter when encountering H2O2. It can also be used for detection of activity of Peroxidase. When adding enhancer, the kit will have…

  • WesternBright™ MCF and MCF-IR

    Advansta

    Complete kit for multicolor fluorescent Western blotting WesternBright MCF visible and near infrared (IR) fluorescent Western blotting kits allow the assay of two proteins at once, increasing the quality and quantity of information that can be gained from a single blot. Assay a control…

  • Fish Gelatin Blocking Buffer

    Amresco

    Grade: Proteomics

  • Gentle Stripping Buffer

    Amresco

    Grade: Ultra Pure

  • Ripa Lysis Buffer

    Amresco

    Grade: Biotechnology

  • PBST Buffer 10x Strength Solution

    Bio Basic Inc.

  • Avant™ Buffer Pouches

    Advansta

    Convenient, pure, fast buffer preparation Advansta's Avant buffer pouches are perfect for standardizing your electrophoresis and Western blotting applications. Dissolve a pouch of pre-measured molecular biology-grade chemicals in 500mL of deionized water to obtain a ready-to-use 1x…

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