Cellomics Technology

Viral Gene Delivery, Cellomics Technology

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  • PGK-GFP lentivirus

    Cellomics Technology

    Green fluorescent protein (GFP), a 27 kDa protein derived from the jellyfish Aequorea victoria, emits green light (emission peak at a wavelength of 509 nm) when excited byblue light (excitation peak at a wavelength of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell…

  • STAT3 Firefly luciferase reporter lentivirus (Puro)

    Cellomics Technology

    This STAT3 reporter lentivirus expresses Firefly luciferase under the control of a minimal (m)CMV promoter and tandem repeats of the STAT3 transcriptional response element (TRE). It can be used to monitor the transcriptional activity of STAT3. Included Fees: C839R71 & C839R72 have a…

  • PGK-Cre, CMV-Firefly luciferase lentivirus

    Cellomics Technology

    Cre Recombinase is a tyrosine recombinase enzyme derived from the P1 Bacteriophage. The enzyme uses a topoisomerase I like mechanism to carry out site specific recombination events. The enzyme (38kDa) is a member of the Integrase family of site specific recombinase and it is known to catalyse the…

  • CMV-MAD2 lentivirus

    Cellomics Technology

    MAD2 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2 was showed to be associated with tumor differentation. This lentivirus express human MAD2 under the control of the CMV promoter.…

  • Inducible tet-on RORB lentivirus

    Cellomics Technology

    RAR-related orphan receptor B (RORB) is a member of the NR1 subfamily of nuclear hormone receptors. Rorb has been shown to be involved in the differentiation of rod photoreceptor cells, Amacrine and horizontal interneurons and associated with bipolar disorder. This lentivirus expresses human RORB…

  • HPSE shRNA lentivirus

    Cellomics Technology

    Heparan sulfate proteoglycans are major components of the basement membrane and extracellular matrix. Heparanase (HPSE) is an enzyme that cleaves heparan sulfate proteoglycans to permit cell movement through remodeling of the extracellular matrix. HPSE is critical regulator of angiogenesis and…

  • Oct4-GFP lentivirus

    Cellomics Technology

    In this lentivirus, the GFP repoter gene is expressed from the Oct4 promoter that is active when cells are in an undifferentiated state. Upon differentiation, the Oct4 promoter is gradually inactivated and therefore the GFP reporter is down-regulated. Oct4-GFP expression is used as a reprogramming…

  • CMV-OCT3/4 lentivirus

    Cellomics Technology

    OCT3/4 (POU5F1) is a transcription factor that is expressed in stem cells and germ cells. Its expression is required to sustain cell self-renewal of undifferentiated stem cells. Oct-3/4 is the most recognized marker for totipotent ES cells. Ectopic expression of OCT3/4 has been used to produce iPS…

  • Inducible tet-on miR-183 lentivirus

    Cellomics Technology

    It has been observed that microRNA miR-183 plays an important role in tumorigenesis and cancer metastasis. This lentivirus expresses miR-183 under the control of the Tet promoter. Included Fees: C839Q57 & C839Q58 have a Dry Ice Fee included in the list price.

  • ADAM-17 shRNA lentivirus

    Cellomics Technology

    ADAM-17 gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family have been implicated in a variety of biologic processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. ADAM-17 is an…

  • Inducible tet-on Smad6 lentivirus

    Cellomics Technology

    Smad6 and Smad7 are inhibitory Smads induced by transforming growth factor β-Smad signal transduction pathways in a negative-feedback mechanism. Smad6 promotes neuronal differentiation in the intermediate zone of the dorsal neural tube. Smad6 is also involved in multiple tissue differentiation…

  • CHI3L1 shRNA lentivirus

    Cellomics Technology

    In humans, CHI3L1 encodes Chitinase-3-like protein 1 (CHI3L1) that a glycoprotein member of the glycosyl hydrolase 18 family. It is a secreted glycoprotein and catalyzes the hydrolysis of chitin glycopolymer found in insect exoskeletons and fungal cell walls. The protein lacks chitinase activity…

  • EF1A-C-MYC lentivirus

    Cellomics Technology

    MYC (C-MYC) is a regulator gene that codes for a transcription factor is believed to regulate expression of 15% of all genes. C-MYC is one of the genes that has been used to generate iPS cells in culture. Human elongation factor-1 alpha (EF-1 alpha) is a constitutive promoter of human origin…

  • Inducible tet-on NEUROD1 lentivirus

    Cellomics Technology

    NEUROD1 is a member of the NeuroD family of basic helix-loop-helix (bHLH) transcription factors. The protein forms heterodimers with other bHLH proteins and activates transcription of genes that contain a specific DNA sequence known as the E-box. It regulates expression of the insulin gene, and…

  • Inducible tet-on CRX lentivirus

    Cellomics Technology

    CRX is a homeodomain transcription factor and one of the key regulator genes of photoreceptor formation. CRX play an important role in transdifferentiation of photoreceptor cells. This lentivirus expresses human Crx under the control of the Tet promoter. Included Fees: C839Q43 & C839Q44…

  • Inducible tet-on C-MYC lentivirus

    Cellomics Technology

    MYC (C-MYC) is a regulator gene that codes for a transcription factor is believed to regulate expression of 15% of all genes. C-MYC is one of the genes that has been used to generate iPS cells in culture. This lentivirus expresses C-Myc under the control of the Tet promoter. Included Fees: …

  • CMV-SIX3 lentivirus

    Cellomics Technology

    SIX3 is a member of the sine oculis homeobox transcription factor family and plays a role in eye development. Mutations in SIX3 have been associated with holoprosencephaly type 2. This lentivirus expresses human SIX3 under the control of the CMV promoter. Included Fees: C839R33 & C839R34…

  • CIAPIN1 shRNA lentivirus

    Cellomics Technology

    In humans, CIAPIN1 (Cytokine Induced Apoptosis Inhibitor 1) encodes a protein called Anamorsin. Expression of CIAPIN1 is dependent on growth factor stimulation. It has no relationship to apoptosis regulatory molecules of the BCL2 or CASP families. Its related pathways include Metabolism and…

  • CMV-LHX2 lentivirus

    Cellomics Technology

    LHX2 (LIM Homeobox 2) belongs to a large protein family, members of which carry the LIM domain, a unique cysteine-rich zinc-binding domain. LHX2 is involved in neuronal differentiation. This lentivirus expresses human LHX2 under the control of the CMV promoter. Included Fees: C839R21 &…

  • CMV-LacZ lentivirus

    Cellomics Technology

    LacZ catalyzes the hydrolysis of the disaccharide lactose, into gluclose and galactose. The use of LacZ as a reporter gene is due to its ability to cleave the bond between galactose and the indole, 5-bromo-4-chloro-3-hydroxyindole. The cleaved indole, is an easily detectable and insoluble blue…

  • HPSE shRNA lentivirus

    Cellomics Technology

    Heparan sulfate proteoglycans are major components of the basement membrane and extracellular matrix.Heparanase (HPSE) is an enzyme that cleaves heparan sulfate proteoglycans to permit cell movement through remodeling of the extracellular matrix. HPSE is critical regulator of angiogenesis and tumor…

  • Inducible tet-on SOX9 lentivirus

    Cellomics Technology

    SOX9 is a transcription factor essential for multipotent neural stem cell (NSC) formation and maintenance. Sox9 is a major molecular component of the neuron–glia switch in the developing spinal cord. SOX9 also plays a key role in chondrogenesis. This lentivirus expresses human SOX9 under the…

  • Inducible tet-on Hnf4a lentivirus

    Cellomics Technology

    Hnf4a is a nuclear transcription factor which binds DNA as a homodimer. Hnf4a controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver,…

  • EF1A-HLF lentivirus

    Cellomics Technology

    Hepatic Leukemia Factor (HLF) is a member of the proline and acidic-rich (PAR) family, a subset of the bZIP transcription factors. Chromosomal translocation of this gene with the E2A gene cause a subset of childhood B-lineage acute lymphoid leukemia. This lentivirus expresses human HLF under the…

  • Inducible tet-on PAX6 lentivirus

    Cellomics Technology

    Pax6 gene contains a paired domain and a paired-type homeodomain and is expressed during early neuroretina development. Pax6 is required for multipotency of retinal progenitors. Tansfection of Pax6 into ES or iPS cells induces retinal progenitors. Pax6 alone is sufficient to induce…

  • hTERT lentivirus (CMV, Puro)

    Cellomics Technology

    Telomerase reverse transcriptase (TERT, or hTERT in human) is a catalytic subunit of the enzyme telomerase, Ectopic expression of telomerase in telomerase-silent cells is sufficient to overcome senescence and to extend cellular lifespan, and has been used for cell immortalization. This lentivirus…

  • CMV-rtTA lentivirus (Neo)

    Cellomics Technology

    This strain carries the tetracycline reverse transcriptional activator (rtTA) under the control of the CMV promoter. The rtTA binds to tetracycline operator elements (TetO) in the presence of doxycycline, causing transcriptional activation of genes downstream of TetO. Included Fees: C839Q29…

  • CXCL1 shRNA lentivirus

    Cellomics Technology

    In humans, CXCL1 gene encodes a small chemokine (C-X-C motif) ligand 1 (CXCL1) cytokine belonging to the CXC chemokine family that was previously called GRO1 oncogene, GROα, KC, neutrophil-activating protein 3 (NAP-3) and melanoma growth stimulating activity, alpha (MSGA-α). CXCL1 plays…

  • Heat Inducible GFP lentivirus

    Cellomics Technology

    Green fluorescent protein (GFP), a 27 kDa protein derived from the jellyfish Aequorea victoria, emits green light (emission peak at a wavelength of 509 nm) when excited byblue light (excitation peak at a wavelength of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell…

  • Inducible tet-on NR2E3 lentivirus

    Cellomics Technology

    NR2E3, a photoreceptor-specific orphan nuclear receptor, is believed to play a pivotal role in the differentiation of photoreceptors. Mutations in NR2E3 result in syndromes characterized by excess blue cones and loss of rods: enhanced S-cone syndrome (ESCS). This lentivirus expresses human NR2E3…

  • Set of lentivirus (EF1A-driven LIN28, NANOG, SOX2, OCT3/4, KLF4 and C-MYC)

    Cellomics Technology

    This set contains six EF1A-driven vectors: LIN28 (PLV-10015), NANOG (PLV-10014), SOX2 (PLV-10013), OCT3/4 (PLV-10012), KLF4 (PLV-10011) and C-MYC (PLV-10010). Human elongation factor-1 alpha (EF-1 alpha) is a constitutive promoter of human origin that can be used to drive ectopic gene expression in…

  • Calcium indicator GCaMP3 lentivirus (CMV)

    Cellomics Technology

    Calcium is a universal second messenger regulating essential cellular signaling events in a broad range of tissues and organisms. GCaMP3 is a fusion calcium indicator of green fluorescent protein (GFP), calmodulin and the M13 domain of a myosin light chain kinase designed to show the calcium ion…

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