Endo Agar was developed by Endo to differentiate gram-negative bacteria on the basis of lactose fermentation, while inhibiting gram-positive bacteria (1). Inhibition of the later was achieved without the use of bile salts as was traditionally used. Endo was successful in inhibiting gram-positive…

Endo (1) had first developed a culture medium for differentiation of lactose fermentors and non-fermenters and further developed as todays Endo Agar (2). Endo agar is used for microbiological examination of potable water and waste water, dairy products and food (3,4,5). Sodium sulphite and…