Protein Purification

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Protein Purification

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  • Focus™

    bioWORLD

    FOCUS™ Cytoplasmic/Nuclear Protein Extraction (Thomas No. C994C79) Fractionation method is based on gentle lysis of the sample followed by centrifugation to collect and separate nuclei from the rest of the cytoplasmic proteins. Kit contains reagents necessary for fractionation and…

  • Lysis Buffer

    Enzymatic lysis of gram-negative bacteria. Gram-negative bacteria are less susceptible than gram-positive bacteria to lysoyme and detergents. Enzymatic lysis of gram-negative bacteria requires the outer membrane to be permeabilized to expose the peptidoglycan layer to attack. The Bacillus…

  • Q-Cons™ Rapid Concentrators

    bioWORLD

  • Cellbuster-A™ and Cellbuster-B™ Kits

    bioWORLD

    CellBuster-A Kit (Animal cell protein prep.) An efficient cell lysis medium for animal cells for preparation of total cellular proteins. Cell lysis facilitated by combination of proprietary detergent mixture. The kit contains enough reagents for 50 medium scale cultures. …

  • Abundant prot removal-haptoglobin A..

    bioWORLD

    The BIO-WORLD Abundant Protein Removal-Haptoglobin kit removes the high-abundant protein- Haptoglobin from human serum and plasma. The high affinity-based column contains separopore-matrix coupled with antibodies against the serum protein. The presence of abundant proteins in human serum and…

  • Abundant protein removal-HSA Kit

    bioWORLD

    The bio-World Abundant Protein Removal-HSA kit removes the high-abundant protein- HSA from human serum and plasma. The high affinity-based column contains Separopore®-matrix coupled with antibodies against the serum protein. The presence of abundant proteins in human serum and plasma often…

  • Glutathione, reduced

    bioWORLD

    A tri-peptide involved in sulfur metabolism High Purity Grade Moisture: Iron: Lead: (GSH; g-Glu-Cys-Glu)

  • Ni-Chelated Separopore® 4B Agarose

    bioWORLD

    Ni-Chelated Separopore® 4B is an imminodiacetate (IDA)-immobilized metal affinity chromatography (IMAC) matrix that seperates proteins via their differences in affinity to nickel metal ions. Properties Imminodiacetate immobilized matrix charged with Ni metal ions. IIdeal for…

  • Abundant Prot Removal – a-1-antitry..

    bioWORLD

    The bio-World Abundant Protein Removal a-1-antitrypsin kit removes the high-abundant protein a-1-antitrypsin from human serum and plasma. The high affinity-based column contains Separopore® matrix coupled with antibodies against a-1-antitrypsin. The presence of abundant proteins in human…

  • Dissolve-away Kit

    bioWORLD

    DissolveAway is for solubilization of inclusion bodies in protein expression studies in bacteria Minimizes denaturation of proteins Increases efficiency and yield of your recombinant protein FoamFree ( Thomas No. C994H72 ) is recommended for use in large preparations

  • Q-Cons™Rapid Protein Concentrators

    bioWORLD

    Quick Concentrators™ for proteins. Prepacked columns of Q-Cons™, just add diluted proetein and spin, then collect concentrated sample. Concentrate proteins 5 to 20 fold in 5 minutes. 5ml column Concentrates 4 ml volume

  • Yeast Lysis Solution

    bioWORLD

    Common solution for protein and DNA isolation from yeast. Technical Information: LiCl (2.5M) Tris-HCl, pH 8.0 (50mM) Triton X 100 (1%) EDTA (62.5mM)

  • Protein Kits

    bioWORLD

    Uppa-PROTEIN-Concentrater Kit, Micro (Thomas No. C994C97) Protein solution as dilute as 1 ng/ml can be quantitatively concentrated by using the Universal Protein Precipitation reagent followed by solubilization and centrifugation through a Gel filtration spin column (provided). Concentrates…

  • G-50 Packed Columns, 50-150 µl

    bioWORLD

    Macro-spin columns have a packing volume of 500 μl suitable for centrifugation of 50-150 μl sample volume. Prepacked with Sephadex G-50 Collect DNA with small MW (10-20 bases) Suitable for use with Swing-out Rotor Centrifuge No High Speed needed avg, speed needed is 1000-2000…

  • GST-Tagged Protein Purif Buffer

    bioWORLD

    bio-World's GST-Tagged Protein Purification Buffer Kit consists of a set of all buffers necessary for OnePass™ GST-Tagged Recombinant Protein Purification System (Glutathione-Separopore ® conveniently prepacked spin columns for gravity flow chromatography) or bath…

  • Trypsin-digestion Mix

    bioWORLD

    Lysis buffer for extracting proteins from cells. Used for efficient digestion of proteins with trypsin Modified Mass- Spec grade for use with the In-gel extraction kit Sufficient for 100 in gel digestions

  • AuraView™

    bioWORLD

    AuraView™ colloidal gold for weste AuroView™ is a new X-ray contrast agent for research and animal use, based on Nanoprobes' gold nanoparticle technology. 15-nm gold nanoparticles containing 100 mg gold metal Ready to be reconstituted in water, PBS, or other buffer

  • Foam Free

    bioWORLD

    Foam Free, free-flowing dry powder, has been specifically developed for controlling foam in various biotechnology applications Prevents foaming during sonication or homogenization of cells and tissues for protein extraction, in hybridization solutions during rocking and shaking, and during…

  • Q-Cons™ Rapid Concentrators

    bioWORLD

    Quick Concentrators™ for proteins. Prepacked columns of Q-Cons™, just add diluted proetein and spin, then collect concentrated sample. Concentrate proteins 5 to 20 fold in 5 minutes. 25 ml column Concentrates 15 ml volume

  • ProPur Rapid Purification Kits & Buffers

    BD

    Ready to use Rapid purification Choice of three chemistries Kits are designed to comprise all necessary components. They allow you to choose the binding capacity and sample volume you need. The three chemistries - Protein A, Protein G for antibody purification and Metal Chelate…

  • Peptide Precleaning Buffer

    bioWORLD

    For clearification of proteins to eliminate nucleic acids and polysaccharides before electrophoresis. Total protein preparation and cleaning solution. For use with 1D gels Storage: -20C Sold as 10 - 1 mL quantities of ready-to-use solution each

  • Triton Lysis Buffer

    Lysis buffer for extracting proteins from cells. 1X concentration Tris-HCl, pH 7.4 (50mM) NaCl (150mM) Triton X-100 (1%) EDTA (5mM)

  • Denaturing Lysis Buffer

    Lysis buffer for extracting proteins from cells. 1X concentration Tris-HCL, pH 7.4 (50mM) SDS (2%)

  • Protein pur-E.coli lysis Buffer

    Sample buffer for HIS-TAG protein purification from E.coli and insect cells. pH 8.0 Na2HPO4 (50mM) NaCl (300mM) Imidazol (10 mL)

  • Carrier Ampholytes

    Carrier Ampholytes 40% w/v solution in water. Analytical Grade Used for Isoelectric focusing (IEF) and 2D gel analysis. 10 mL

  • OnePass™ GST-Tagged Protein Purif

    bioWORLD

    "bio-World's OnePass ™ GST Tagged Protein Purification Kit consists of Glutathione Separopore ® (Glutathione reduced coupled to 4% cross-linked agarose beads), designed for the single-step affinity purification of proteins with a glutathione S-transferase…

  • Abundant prot removal-transferrin K..

    bioWORLD

    The presence of abundant proteins in human serum and plasma often masks the detection of low-abundant proteins of interest that serve as biomarkers for disease when being studied by 2D gel electrophoresis or LC/MS. After depletion of these high-abundant proteins, serum samples consist of an…

  • EIA Buffer, 10x solution

    A 1M Phosphate solution containing BSA, NaCl, EDTA, and Azide. Product contains 50mL of 10X solution concentrate and can be diluted with distilled water to create 500mL or 1X solution. pH 7.4 (10mM) Stability >1 year at 4° C

  • Poly-His Ab affinity purifiction kit

    bioWORLD

    Polyclonal antibodies (Rabbit) against polyHistidine are immobilized on Separopore ® (4% beaded agarose) to prepare a column for purification of His-tagged proteins using affinity chromatography. Bound protein is eluted using low-pH non-denaturing conditions. Kit includes all…

  • Protein pur-mam-elution Buffer, pH 8

    Sample buffer for HIS-TAG protein purification from mammalian cells. pH 8.0 Na2HPO4 (50mM) NaCl (300mM) Imidazol (250mM) Tween-20 (0.5%)

  • Protein pur-E.coli elution Buffer

    Sample buffer for HIS-TAG protein purification from E.coli and insect cells. pH 8.0 Na2HPO4 (50mM) NaCl (300mM) Imidazol (250mM)

  • Protein pur-mam-wash Buffer,pH 8.0

    Sample buffer for HIS-TAG protein purification from mammalian cells. pH 8.0 Na2HPO4 (50mM) NaCl (300mM) Imidazol (20 mL) Tween-20 (0.5%)

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