Protein

Isolation & Purification, Protein

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Protein Isolation & Purification

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  • Total Protein Cell Lysis Buffer

    Amresco

    Grade: Proteomics Easy-to-use lysis buffer for extraction of total cell protein. Total Cell Protein Lysis Buffer contains a non-ionic detergent as the lysis agent to reduce denaturation.

  • Nickel Chelating Resin

    G-Biosciences

    Immobilized metal affinity chromatography (IMAC) resin utilizing nickel (Ni2+) for the purification of 6x histidine tagged proteins. This resin binds to six histidine residues (6x His), a common tag used in protein purification. The resin consists of iminodiacetate coupled to 6% cross-linked…

  • Pierce™ Premium Grade Sulfo-NHS-LC-Biotin

    Thermo Scientific Pierce

    Thermo Scientific Pierce Premium Grade Sulfo-NHS-LC-Biotin is our highest quality formulation of this popular amine-reactive biotinylation reagent, specially characterized for applications where product integrity and risk minimization are paramount.

  • FastPROTEIN™ Blue Matrix

    MP Biomedicals

    The FastPROTEIN Blue Kit is designed for rapid isolation and purification of protein from bacterial cultures. The FastPROTEIN Blue Matrix, also known as Lysing Matrix B, is used with any FastPrep® Instrument to reproducibly lyse gram positive and gram negative bacterial cells within…

  • FastPROTEIN™ Red Matrix

    MP Biomedicals

    The FastPROTEIN Red Kit is designed for rapid isolation and purification of protein from Yeast cultures. The process is designed to be a small scale test for protein induction. The FastPROTEIN Red Matrix, also known as Lysing Matrix C, is used with the any FastPrep® Instrument to…

  • Mitochondrial Protein Isolation Buffer

    Amresco

    Grade: Proteomics A single component mitochondrial protein isolation buffer for enrichment of mitochondrial proteins from cultured mammalian cells. Entire extraction procedure takes less than an hour.

  • Protein Precipitation Kit

    Amresco

    Removal of interfering salts, detergents, and buffers Reproducible and consistent results This Protein Precipitation Kit utilizes a simple trichloroacetic acid (TCA) based method to precipitate proteins in complex mixtures. Only protein in the sample is pelleted, while interfering salts and…

  • Cytoplasmic/Nuclear Protein Enrichment Kit

    Amresco

    Grade: Proteomics The Cytoplasmic/Nuclear Protein Enrichment Kit generates distinct nuclear and cytoplasmic fractions from cultured cells in 1.5 hours. The kit includes 3 buffers that fractionate both suspension cultures and adherent cells with minimal cross-contamination.

  • RunBlue™ Prestained Markers

    Expedeon

    RunBlue prestained markers enable easy visualization of the marker proteins during and after running and western blotting. The markers can be used to estimate the molecular weight of your protein of interest and assess the transfer efficiency during blotting. Expedeon offers two types of prestained…

  • Amintra Protein G Resin

    Expedeon

    Amintra Protein G resin; a fast flow sepharose matrix covalently modified with recombinant Protein G expressed in E. coli. The albumin domain naturally present in these proteins has been deleted to create a resin with a high capacity and affinity for antibodies. Typically binding capacity of 25 mg…

  • Amintra Protein L Resin

    Expedeon

    Protein L is an immunoglobulin-binding protein that was expressed, isolated from the bacterial species Peptostreptococcus magnus.  Protein L Agarose resin offers a unique ability in a convenient way to separate a wider range of mammalian Immunoglobulin classes and sub-classes compare to…

  • RunBlue™ LDS Sample Buffer

    Expedeon

    RunBlue LDS Sample Buffer should be used to prepare all samples for running on RunBlue Precast Gels. Specifically formulated for the RunBlue buffer system to provide the best running conditions and results.

  • RunBlue™ DTT Reducer

    Expedeon

    RunBlue DTT Reducer is a reducing agent which should be added during sample preparation if you wish to perform reduced SDS-PAGE.

  • Amintra Protein A Resin

    Expedeon

    Amintra Protein A resins are designed on a highly crosslinked sepharose matrix covalently modified with recombinant Protein A and Protein G expressed in E. coli. The albumin domain naturally present in these proteins has been deleted to create a resin with high capacity and affinity for antibodies.

  • FASP™ Protein Digestion Kit

    Expedeon

    Complete Protein Solubilization. Complete Trypsin Digestion. Every Time. Now you can use aggressive, whole proteome solubilization protocols and reagents to prepare proteins from any biological material for trypsin digestion and analysis by mass spectrometry. Based on the Universal Sample…

  • RunBlue™ Run & Blot Systems

    Expedeon

    Expedeon's RunBlue Run & Blot system provides you with a gel tank with unsurpassed functionality. The core module hinges open to accept gel cassettes or buffer dam and then simply clips in place to provide a completely sealed cathode chamber. This allows RunBuffer to be added and samples to…

  • UPX™ Universal Protein Extraction Kit

    Expedeon

    The UPX Universal Protein Extraction Kit is for researchers who need universal, unbiased extraction of both membrane proteins and soluble proteins, and an abundance of starting material. One Protein Extraction Protocol for Tissues and Cells, Fresh or Frozen Expedeon's UPX Protein…

  • GELFREE® 8100 Cartridge Kits

    Expedeon

    Ready-to-use Gelfree 8100 Cartridge Kits complement the Gelfree 8100 Fractionation Work Station. Each Cartridge Kit contains pre-formulated Running and Sample Buffers, as well as the Fractionation Cartridge. The Gelfree 8100 Fractionation Cartridge contains eight independent channels for molecular…

  • RunBlue™ Tris-Glycine Blot Buffer

    Expedeon

    RunBlue Tris-Glycine Blotting Buffer is formulated for use with Expedeon Precast SDS-PAGE Gels. Dilution of RunBlue TG Buffer varies between the various blotting aparatuses.

  • RunBlue™ Native Run Buffer

    Expedeon

    RunBlue Native RunBuffer is optimised for use with RunBlue Precast Gels to provide the best running contitions and optimal sample resolution. Based on a Tris-Tricine buffer system this gives improved resolution at lower molecular weights whilst retaining excellent resolution at the high moleculr…

  • Stabil-PAC

    Expedeon

    Each Stabil-PAC kit contains NVoy technology designed to increase the solubility and stability of proteins whilst preventing aggregation and reducing non-specific binding. Stabil-PAC kits are a convenient way to test if NVoy is able to assist your work. These packs contain single shot amounts of…

  • Avidin

    G-Biosciences

    Avidin is a glycoprotein with approximately 10% of its total mass comes from carbohydrates. Avidin has a molecular weight of 67kDa and contains four identical 128 amino acid subunits that each have a single biotin binding domain. Avidin is a basic protein with an isoelectric pH of 10-10.5 and is…

  • Amintra Glutathione Resin

    Expedeon

    Amintra's Glutathione resin is designed for rapid one-step purification of glutathione S-transferase-tagged proteins from bacteria, yeast, insect and mammalian cultures. Glutathione is covalently coupled to a high crosslinked agarose matrix that provides a high chemical and physical stability…

  • RunBlue™ Native Sample Buffer

    Expedeon

    All RunBlue Buffers, Reagents and Accessories have been specifically designed and formulated for use with the RunBlue gels and apparatus to provide optimum performance and results. Buffers are made using the highest quality chemicals and have different compositions to other manufacturers buffers.

  • Amintra Protein A Magnetic Resin

    Expedeon

    Protein A is immobilised to highly porous and highly cross-linked agarose incorporating magnetic material. The particles have an open pore structure with excellent mass transfer properties for large protein molecules. Amintra Protein A Magnetic can be used in any commercially available magnetic…

  • YPX™ Yeast Protein Extraction Kit

    Expedeon

    A Simplified Protocol for Higher Yield Extraction Any Morphology. Any Medium. Complete Solubilization. Expedeon's YPX Yeast Protein Extraction Kit is for researchers who need fast, high-yield, unbiased yeast protein extraction and the ability to handle many samples at once. The…

  • FFPE-FASP™ Protein Digestion Kit

    Expedeon

    Filter-Aided Sample Prep (FASP) is the enabling technology behind quantitative mass spectrometry analysis of archived tissues. The resulting filtrate is free of detergents, large molecules, and other substances that would interfere with mass spectrometry analysis of the proteome. Contents:…

  • Amintra NI-NTA Resin

    Expedeon

    Expedeon's Ni-NTA affinity resin is designed for simple, rapid His-tagged recombinant protein purification from a cell lysate under native or denaturing conditions. Amintra Ni-NTA offers excellent flow properties and high protein binding capacity: in excess of 50mg protein/ml. NTA is a…

  • Amintra NHS-Activated Resin

    Expedeon

    Amintra NHS-activated is a highly cross-linked N-hydroxysuccinimide activated matrix. Ligands containing primary amino groups are coupled directly to this active ester to form a chemically stable amide bond.

  • RunBlue™ Gel Drying Solution

    Expedeon

  • NVoy Polymers

    Expedeon

    NVoy is a large, uncharged polymer based on a polysaccharide backbone with selected hydrophobic side chains substituted onto the backbone at specific residues to create an amphipathic molecule. NVoy is used in place of detergents, fusion proteins, arginine, chaperones and other common additives…

  • pIMAGO-biotin

    Expedeon

    pIMAGO is a universal phosphoprotein detection technology that enables sensitive and specific recognition of phosphorylated molecules. Unlike phospho-antibodies binding is not biased by amino acid sequence, and can therefore be used for detection of any phosphorylation event on any protein site.…

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