Miller's LB broth is based on the original Lysogeny Broth (LB) media formulation developed by Luria and Bertani, which was designed for the rapid propagation of recombinant E. coli strains.  Miller's LB contains a 20-fold concentration sodium chloride compared to the original…

Technical Advantage:   This is the preferred medium for post-transformation E. coli work S.O.C. medium is used as a recovery medium during the final stage of transformation of E. coli.  The use of SOC medium improves transformation efficiency. Complete Certificate of…

Corning Transport Medium is formulated using the final ingredient concentrations outlined in CDC's SOP#: DSR-052-01 “Preparation of Viral Transport Medium”. Transport Medium is an isotonic solution at neutral pH containing protective proteins, antibiotic to prevent microbial…

IPTG (isopropylthiogalactoside) is an inducer of the lac operon in bacteria, which is frequently used in cloning as a component of a recombinant plasmid.  When used with X-gal to detect lac gene activity, IPTG can be used for screening of clones containing a gene of interest in the lac operon.…

X-Gal (5-bromo-4-chloro-3-indolyl-b-D-galactopyranoside) is a substrate of b-galactosidase, a gene product of the lac operon in E. Coli.  X-Gal included in bacterial medium will be hydrolyzed by ß-galactosidase to produce indoxyl, a colormetric indicator of the activity of the lac…

Technical Advantage:   Can be autoclaved, unlike ampicillin Carbenicillin is a form of penicillin and is often used in place of ampicillin because it maintains stability after being autoclaved.  Like ampicillin, carbenicillin interferes with cell-wall synthesis of actively growing…