These media are used for the differentiation between Enterobacteriaceae and the members of aerogenes group on the basis of citrate utilization as sole carbon source. Initially the citrate medium was developed by Koser (1) containing ammonium salt as the only nitrogen source and citrate as the only…

Phenylethanol Agar Base medium is utilized for the selective isolation of gram-positive bacteria. This medium can be supplemented with 5 % sheep blood. This medium is especially useful when specimens are contaminated with swarming Proteus species. It is also useful in the diagnostic studies of…

Leibovitz's Medium was specifically designed to grow cells in a CO2 free atmosphere. The standard sodium bicarbonate/CO2 buffering system is replaced by combination of free basic amino acids, phosphate buffers and higher levels of galactose and sodium pyruvate. As a result, the medium does not…

An enrichment and antibiotic supplement recommended for selective isolation of nonsporing anaerobic bacteria. Storage and Shelf Life Store at 2 - 8°C. Use before expiry date on the label.

Anaerobic bacteria live in an oxygen-free environment. Some anaerobic bacteria actually die if oxygen is present while others fail to grow and multiply (1). One of the methods of cultivation of anaerobes is using the Sprays medium by using the anaerobic culture dish (2). Liver Veal Agar is…

Bio Peptone, Certified is manufactured by a controlled enzymatic hydrolysis of casein and animal tissue. It provides a broad spectrum of peptides and amino acids which supports better microbiological growth characteristics to a large variety of organisms. Appearance Light yellow to…

Luria Bertani Agar is prepared as described by Lennox (1) for cultivation and maintenance of recombinant strains of Escherichia coli . Luria Bertani Agar, Miller (2) is slightly different with double amount of sodium chloride. The media is nutritionally rich for the growth of pure cultures of…

Granulated TCBS Agar is for selective isolation and cultivation of Vibrio cholerae and other enteropathogenic Vibrios causing food poisoning.

For the cultivation and enumeration of Clostridia from pharmaceutical products in accordance with the microbial limit testing by harmonized methodology of USP/EP/BP/JP.

Minimum Essential Medium is a modification of Basal Medium Eagle (BME). It was developed by Harry Eagle to meet the specific nutritional requirements of certain subtypes of Hela cells and normal mammalian fibroblasts. MEM includes higher concentration of amino acids so as to closely approximate the…

Clostridium perfringens food poisoning is one of the common types of human food borne illness. C. perfringens is found in raw meats, poultry, dehydrated soups and sauces, raw vegetables and other foods and food ingredients, but occurrences of foodborne illness are usually associated with cooked…

It is used for carbiohydrate fermentation studies and for studies of haemolytic activity of Clostridia, Streptococci and other organisms. Casein enzymic hydrolysate is the only source of nutrient present. Other peptones, rich in carbohydrates like yeast extract or papaic digest of soyabean meal are…

HiCrome UTI Selective Agar is chromogenic differential medium for identification, differentiation and confirmation of enteric bacteria from specimens such as urine, which may contain large number of Proteus species as well as potentially pathogenic gra

It is rich in carbohydrates and is ideally suitable for use in media employed for cultivation of fungi such as, Malt Agar, Malt Extract Agar, Yeast Malt Agar, etc. Malt Extract Powder is prepared from aqueous extract of sprouted malt grains and dried at low temperature to preserve nutrients…

EC Medium is used for detection of coliforms during bacteriological examination of water, milk and foods. It was originally described by Hajna and Perry (1). This medium was later used by Fishbein and Surkiewicz to carry out Escherichia coli confirmatory tests (2). It is also used in MPN methods…

Malonate Broth is recommended for differentiation of Enterobacter and Escherichia on the basis of malonate utilization. Malonate utilizers change the color of the medium to blue due to bromothymol blue, pH indicator in the medium. Enterobacter aerogenes, Klebsiella pneumoniae, Salmonella Arizonae…

Azospirillum Medium w/ 0.17% Agar (Twin Pack) is recommended for cultivation of Azospirillum species. Azospirillum species grows luxuriantlly in presence of Malic acid. Dipotassium phosphate provides buffering effect and other inorganic salt ingredients provide necessary growth nutrients. Agar at…

Fraser Broth Base, Modified (Half Fraser Broth) is recommended for the selective enrichment of Listeria species from food. Listeria species hydrolyze esculin to glucose and esculetin. The latter combines with ferric ions of ferric ammonium citrate, resulting in the formation of 6-7…

Hiveg media Formulated as described by Buchbinder et al which is recommended by APHA and FDA. for the determination of plate counts of microorganisms in food, water, waste water and also from clinical samples. Application: For Determination of Plate Counts of Microorganisms in Food, Water, Waste…

Endo had first developed a culture medium for differentiation of lactose fermenters and lactose non-fermenters while inhibiting gram-positive bacteria (1). Inhibition of the later was achieved without the use of bile salts as was traditionally used. Endo was successful in inhibiting gram-positive…

Acetamide Agar is formulated as per the recommendation of Standard Methods for the Examination of Water and Wastewater (1). Gilardi and others showed that a wide variety of non-fermenting organisms were capable of utilizing acetamide by using basal mineral media (2, 3). However very few organisms…

HiEncap™ Potato Dextrose agar is recommended for the isolation and enumeration of yeasts and molds. Thomas No. C870N22 Each capsule contains 9.75 grams of medium. Suspend 1 capsule in 250 ml (4 capsules in 1000 ml) distilled or purified water. Thomas No. C870N23 Each capsule contains…

Limabean agar is recommended for cultivation of phytopathological and other fungi. It is composed of an infusion of dry lima beans and agar. Limabean infusion provides all essential growth nutrients for fungi. The pH of the medium is 5.6, which enables luxuriant fungal growth. Application: For…

Plate Count Agar is equivalent to the medium recommended by APHA for the isolation of microorganisms in milk and other dairy products (1). Casein enzymic hydrolysate provides amino acids and other complex nitrogenous substances. Yeast extract supplies Vitamin B complex. APHA recommends pour…

Nutrient media are basic culture media used for maintaining microorganisms, cultivating fastidious organisms by enriching with serum or blood and are also used for purity checking prior to biochemical or serological testing (1, 2). Nutrient Broth has the formula originally designed for use in the…

Carbohydrate Consumption Broth Base is recommended for cultivation and differentiation of Listeria species. Similar medium is also recommended by FDA and ISO different iconcentration of Bromocresol purple. Differentiation is based on fermentation of glucose, xylose, rhamnose, ribose,…