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GE Healthcare GST Tag Small Scale

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  • pGEX Vectors

    GE Healthcare

    A tac promoter for chemically inducible, high-level expression of GST-tagged recombinant proteins An internal lacIq gene for use in any E. coli host Very mild elution conditions for release of fusion proteins from the affinity matrix, thus minimizing effects on antigenicity and functional…

  • PreScission Protease

    GE Healthcare

    PreScission Protease is a genetically engineered fusion protein consisting of human rhinovirus 3C protease and GST. It specifically cleaves between the Gln and Gly residues of the recognition sequence of LeuGluValLeuPheGln/GlyPro. Enables the low-temperature cleavage of fusion proteins…

  • E. coli BL21

    GE Healthcare

    It is an exemplary host for the expression of recombinant proteins. As an E. coli B strain, it further lacks the major protease, encoded by the lon gene, catalyzing the endoproteolytic cleavage of damaged and recombinant proteins in the cell. A protease-deficient E. coli host strain for…

  • Factor Xa

    GE Healthcare

    The Glutathione S-transferase (GST) Gene Fusion System from GE Healthcare is a versatile system for the expression, purification, and detection of GST-tagged proteins produced in E. coli. Enables the site-specific cleavage of fusion proteins containing an accessible Factor Xa protease…

  • Thrombin

    GE Healthcare

    Thrombin is a protease used to digest fusion proteins prepared from pGEX vectors recognition sequence for thrombin (pGEX-1lT, pGEX-2T, pGEX-2TK, pGEX-4T-1, pGEX-4T-2, and pGEX-4T-3). Purified from bovine plasma. Enables the site-specific cleavage of fusion proteins containing an accessible…

  • GST GraviTrap

    GE Healthcare

    GST GraviTrap contains 10 disposable columns prepacked with 2 ml of Glutathione Sepharose 4B, sufficient for purification of up to 20 mg of fusion protein. Convenient, prepacked gravity-flow columns Allows direct purification of both clarified and unclarified cell lysates Yields up…

  • GST SpinTrap Columns

    GE Healthcare

    GST SpinTrap columns are designed for rapid small-scale purification of GST-tagged proteins directly from clarified or unclarified cell lysates. Preserves protein antigenicity and function Yields up to 500 µg of pure protein per column Allows direct purification of both clarified…

  • MultiTrap Filter Plates Prepacked with Sepharose

    GE Healthcare

    GST MultiTrap FF and GST MultiTrap 4B are prepacked, 96-well filter plates for reproducible, high-throughput screening and rapid parallel purification of glutathione S-transferase (GST) tagged proteins. 96-well filter plate format for high-throughput screening and parallel purification of…

  • M13KO7 Helper Phage

    GE Healthcare

    The phage is an M13mp1 derivative containing a modified gene 11[G changed to T at position 6,125, giving a met to ile change at codon 40 of the gene 11 protein (1)], an insertion of the origin of replication of p15A and the kanamycin resistance gene from Tn903 at the Ava I site (position 5,825).

  • GST Detection Module

    GE Healthcare

    GST Detection Module enables sensitive detection of GST tagged proteins and contains components For detection using either a biochemical assay where glutathione and 1-chloro-2-4-dititrobenzene (CDNB) serve as substrates for GST to yield a yellow product detectable at 340 nm, or an immunoassay …

  • Anti-GST Antibody

    GE Healthcare

    Anti-GST Antibody is a polyclonal antibody purified from the sera of goats immunized with purified schistosomal GST, Because of its polyclonal nature, it can recognize more than one epitope on GST. For the highly sensitive and specific detection of recombinant schistosomal GST fusion…

  • GST Vector Primers for Sequencing

    GE Healthcare

    The Glutathione S-transferase (GST) Gene Fusion System from GE Healthcare is a versatile system for the expression, purification, and detection of GST-tagged proteins produced in E. coli. Ready for immediate use in sequencing double-stranded DNA inserted into pGEX vectors 0.05 A260 unit…

  • GST Buffer Kit

    GE Healthcare

    GST Buffer Kit contains carefully prepared buffer concentrates for binding, washing, and elution of GST-tagged proteins according to recommended protocols. The kit contains 10x PBS, reduced glutathione, dilution buffer, and an instruction booklet. Sufficient reagents are supplied to purify up to 20…

  • pEZZ 18 Protein A Gene Fusion Vector

    GE Healthcare

    For rapid expression of secreted fusion proteins and their one-step purification using IgG Sepharose 6 Fast Flow The phagemid pEZZ 18 contains the protein A signal sequence and two synthetic "Z" domains based on the "B" IgG binding domain of protein A. Proteins are expressed…

  • GST Bulk Kit

    GE Healthcare

    GST Bulk Kit is designed for small-scale manual purification of GST-tagged proteins using either column chromatography or batch method. Facilitates optimization studies of expression, solubility conditions and purification parameters of GST-tagged proteins. For rapid purification of…

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