Enzymes & Inhibitors, 1 g
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ABTS (2,2'-Azino-bis(3-ethylben
bioWORLDOne Component Microwell Peroxidase Substrate with excellent performance characteristics, good stability and wide working range. Develops blue-green color measurable between 405 nm and 410 nm.
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Pectolyase Y-23 (A. niger)
MP BiomedicalsPectolyase Y-23 is a highly purified maceration enzyme from Aspergillus niger. It contains two types of pectinase such as endopolygalacturonase and endo-pectin lyase in high activity. Included is a maceration stimulating factor which stimulates tissue maceration by both pectinases. Unit Definition:…
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Lysozyme from Chicken Eggwhite
MP BiomedicalsLysozyme (muramidase) hydrolyzes preferentially the β-1,4 glucosidic linkages between N-acetylmuramic acid and N-acetylglucosamine which occur in the mucopeptide cell wall structure of certain microorganisms, such as Micrococcus lysodeikticus. Extracts group specific antigens …
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Pectinase
bioWORLDAlso known as Poly-(1,4-a-D-galacturonide)glycanohydrolase. Used in plant protoplast preparation to digest cell wall prior to organelle isolation.
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Ribonuclease A from Bovine Pancreas
MP BiomedicalsRibonuclease belongs to pancreatic ribonuclease family. RNase A is an endoribonuclease that attacks at the 3¢ phosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG. Ribonuclease A is used to remove RNA from DNA plasmid preparations…
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Lactase from Aspergillus oryzae
MP BiomedicalsLactase is an enzyme preparation produced by Aspergillus oryzae fermentation. Hydrolyzes Lactose Produces β-D-Galactose and a-D-Glucose One unit will hydrolyze 1.0 µmole of o-nitrophenyl-beta-D-galactoside to o-nitrophenol per minute at pH 4.5 and 30°C …
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Hyaluronidase from Bovine Testes
MP BiomedicalsHyaluronidase is a glycoprotein containing 5% mannose and 2.17% glucosamine, it catalyzes the random hydrolysis of 1,4-linkages between 2-acetamido- 2-deoxy- b-D-glucose and D-glucose residues in hyaluronate. Hyaluronidase from bovine testes is a tetramer consisting of 4 equal subunits with a…
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Collagenase Type 3 from Clostridium histolyticum
MP BiomedicalsCollagenases degrade native helical collagen fibrils. The substrate, collagen, is the major fibrous component of animal extracellular connective tissue: skin, tendon, blood vessels, bone, etc. True collagenase may cleave simultaneously across all three chains or attack a single strand. The enzyme…
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4-(2-Aminoethyl)-Benzenesulfonyl Fluoride Hydrochloride
MP BiomedicalsAEBSF hydrochloride is a covalent binding serine protease inhibitor, belongs to the family of irreversible sulfonyl fluoride inhibitors that block trypsin and chymotrypsin-type enzymes. Similar in structure to the commonly used inhibitor PMSF, AEBSF offers better solubility in water, lower…
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Collagenase from Clostridium histolyticum
MP BiomedicalsCollagenase products are prepared from culture supernatants of C. histolyticum. It is prepared to contain lower tryptic activity to limit damage to membrane proteins and receptors but with normal to above normal collagenase activity. Suggested for pacreatic islet isolation. Crude Collagenases…
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Pectolyase Y-23 from Aspergillus japonicus
MP BiomedicalsPectolyase Y-23 is a highly purified maceration enzyme from Aspergillus japonicus. It contains two types of pectinase such as endopolygalacturonase and endo-pectin lyase in high activity. Included is a maceration stimulating factor which stimulates tissue maceration by both pectinases. Enzyme…
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Pepsin from Porcine Stomach Mucosa
MP BiomedicalsPepsin, an acid protease, contains a proteolytic enzyme. Pepsin contains the "cathepsin" component which has milk curdling activity. It has a broad range of substrate activity and demonstrates an esterase acitivity. It generally attacks peptide bonds. Pepsin is a peptidase used to…
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Trypsin from Beef Pancreas
MP BiomedicalsTrypsin consists of a single chain polypeptide of 223 amino acid residues. Member of the serine protease family Dissolves blood clots in its microbial form Treats inflammation in its pancreatic form Trypsin cleaves peptides on the C-terminal side of lysine and arginine residues.…
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5-Bromo-2'-Deoxyuridine
MP Biomedicals5-Bromo-2’-deoxyuridine is a thymidine analog and useful for the study of DNA synthesis where it is incorporated into DNA in place of thymidine. Measure DNA synthesis Label dividing cells Study cell signaling and processes that induce cell proliferation Used as a mutagen in…
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Trypsin
G-BiosciencesTrypsin is a serine endopeptidase that specifically cleaves peptide bonds on the carboxy side of s-aminoethyl cysteine, arginine and lysine residues. Typically there is little or no cleavage at arginyl-proline and lysyl-proline bonds. This trypsin is purified from bovine pancreas, 1X…
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Proteinase K
G-BiosciencesProteinase K (also protease K, endopeptidase K, peptidase K or Tritirachiumalkaline phosphatase) (EC 3.4.21.64) is a non-specifc, broad spectrum serine protease that is isolated from the saprophytic fungus Tritirachium album. Proteinase K is routinely used for the purification of target…
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Collagenase Type II from Clostridium histolyticum
MP BiomedicalsCollagenases degrade native helical collagen fibrils. Plays an important role in connective tissue metabolism Produced by specific cells involved in repairs and remodeling processes Type II enzyme that contains greater clostripain activity Collagenase is used for collagen…
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Proteinase K
bioWORLDA serine proteinase used to inactivate RNAse and DNAse in RNA and DNA isolation protocols. Useful in the study of membrane and protein structure. Proteinase K is a highly active protease (MW 28,500 Da) isolated from the fungus Tritirachium album . The enzyme exhibits broad specificity on…
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Collagenase Type I from Clostridium histolyticum
MP BiomedicalsCollagenase is a protease which cleaves the triple-helical protein called collagen. There are three types of tissue collagenases, and these belong to the matrix metalloproteinases (MMP) family. Collagenase from Clostridium histolyticum has extensive use in biological studies, where it is used to…
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Papain from Papaya Latex
MP BiomedicalsPapain is a sulfhydryl protease from Carica papaya latex. Cleaves peptide bonds of basic amino acids, leucine, or glycine Hydrolyzes esters and amides One unit will hydrolyze 1.0 µmole of N-alpha-benzoyl-L-arginine ethyl ester (BAEE) per minute at 25°C and pH 6.2 Papain…
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Horseradish Peroxidase from Horseradish Roots
MP BiomedicalsHorseradish peroxidase is used for production of peroxidase conjugated antisera. It is a widely used label for immunoglobulins in many different immunochemistry applications including ELISA, immunoblotting, and immunohistochemistry. HRP can be conjugated to antibodies by several different methods…
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a-Chymotripsin from Bovine Pancreas
MP BiomedicalsA serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met, Leu) on the carboxyl end of the bond. Produced from 3x Crystallized chymotrypsinogen Used to treat pancreatic insufficiency Used in traumatology Chymotrypsin…
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Lipase from Porcine Pancreas
MP BiomedicalsLipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel. Subclass of esterases Catalyzes hydrolysis of emulsified esters of glycerol and long chain fatty acids One unit will liberate 100 µ moles fatty acid per hour…
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4-(2-Aminoethyl) benzenesulfonyl fluoride hydrochloride Specificity: Specific irreversible inhibitor of serine proteases, including chymotrypsin, kallikrein, plasmin, thrombin and trypsin. A stable non-toxic alternative to PMSF. Solubility: Soluble in water Features …
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Niclosamide
MP BiomedicalsAntihelminthic, molluscicide and trypanocidal agent that inhibits mitochondrial oxidative phosphorylation and anaerobic ATP production. Anticancer agent with antiproliferative activity in a broad spectrum of cancer cells. Targets and inhibits multiple signaling pathways, including STAT3, NF-kB,…
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Cellulase (Onozuka R-10)
bioWORLDFrom Trichadema Viride. Enzyme mixture often used in combination with Macerozyme R-10 (M22010) for isolation of plant protoplasts. One unit of Cellulase will liberate 1.0 µmole of glucose from carboxymethyl cellulose. Lyophilized powder Optimum pH range: 4-5
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Ribonuclease A from Bovine Pancreas
MP BiomedicalsRibonuclease A, and to a greater extent its oligomers and some homologs (such as onconase from frogs), have cytotoxic and cytostatic effects, particularly on cancer cells.This attribute has led to the development of onconase as a cancer therapeutic. Ribonuclease A is used to remove RNA from DNA…
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Catalase from Beef Liver
MP BiomedicalsCatalase is an oxidoreductase that catalyzes the conversion of hydrogen peroxide to water and oxygen. Activates the decomposition of hydrogen peroxide Natural antioxidant Used to study roles of reactive oxygen species in gene expression and apoptosis Hydrogen peroxide is a harmful…
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Naproxen
MP BiomedicalsPharmacological agent that reportedly may show activity through ability to inhibit prostaglandin biosynthesis. Non-selective cyclooxygenase (COX-1 and COX-2) inhibitor. Naproxen has been demonstrated to block Aβ fibril growth and form a complex with copper (II).
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a-Chymotrypsin 1X Crystallized from Bovine Pancreas
MP Biomedicalsα-Chymotrypsin preferentially catalyzes the hydrolysis of peptide bonds involving L-isomers of tyrosine, phenylalanine, and tryptophan. It also readily acts upon amides and esters of susceptible amino acids. In addition to bonds involving aromatic amino acids, chymotrypsin catalyzes at a high…
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Cellulase from Trichoderma virde
MP BiomedicalsCellulase refers to a group of enzymes which, acting together, hydrolyze cellulose. The b-glucosidases, including cellobiose, are primarily active on the smaller molecular weight cellulose hydrolysates. During cellulose breakdown they are active on the dimers and oligomers of cellulose. …
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Proteinase K from Tritirachium album
MP BiomedicalsProteinase K is a highly active stable endopeptidase with a broad spectrum of action was isolated by E. Merk's Darmstadt Biochemical Research Department in 1970 from a culture filtrate of the fungus, Tritirachium album Limber. This fungus is able to grow on Keratin (e.g., wool, horn particles)…