RPI (Research Products International) Chemicals Enzymes and Inhibitors
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ONPG
RPI (Research Products International)Colorimetric and spectrophotometric substrate for detection of β-D-Galactosidase.
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X-GlcA Cyclohexylammonium Salt
RPI (Research Products International)Histochemical substrate for β-D-Glucuronidase (GUS) encoded by the gusA gene. The substrate is used as a qualitative histochemical marker of specific GUS expression in cells and tissue. X-GlcA is cleaved by GUS at the B1 glucuronic bond between glucuronic acid and the…
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X-a-Gal
RPI (Research Products International)Chromogenic substrate for detection of a-galactosidase activity in yeast Saccharomyces cerevisiae. Used in yeast Two-Hybrid System to detect protein-protein interactions within random libraries. The enzyme a-galactosidase enabels yeast to use disaccharide melibose as a carbon source during growth…
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Phosphatase Inhibitor Cocktail III
RPI (Research Products International)Lyophilized blend of four phosphatase inhibitors for use with animal tissue estracts. Reconstitute with 1ml water to produce a 1 ml concentrate. Contains: Sodium Fluoride Sodium Orthovanadate Sodium Pyrophosphate, decahydrate B-Glycerophosphate Specificity: …
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X-GAL & IPTG Combo
RPI (Research Products International)Special savings for ordering X-GAL and IPTG at the same time! Package includes 1 gram bottle of X-Gal and a 1 gram bottle of IPTG.
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RPI 4-Nitrophenyl Phosphate Disodium Salt Hexahydrate
RPI (Research Products International)The RPI 4-nitrophenyl phosphate disodium salt hexahydrate is suitable for use in research facilities and chemical laboratories to conduct wide range of experiments. It is available in sturdy bottle that endures heavy and rigorous usage. Application: Research or Further Manufacturing use only
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Proteinase K
RPI (Research Products International)From Tritirachium album. Serine protease for protolytic inactivation of nucleases during isolation of DNA and RNA.
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X-GAL
RPI (Research Products International)Chromogenic substrate of β-Galactosidase used in combination with IPTG for detection of β-Galactosidase activity in bacterial colonies. X-GAL is cleaved at the B1-4 bond between galactose and the 5-Bromo-4-chloro-3-indolyl portion of X-GAL by β-Galactosidase via hydrolysis. The…
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Bluo-Gal
RPI (Research Products International)Chromogenic substrate for B-D-Galactosidase. Produces a deeper blue water insoluble color than X-GAL.
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RPI 1-O-Methyl a-D-mannopyranoside [Methyl-a-D-Mannopyranoside]
RPI (Research Products International)The RPI methyl-a-D-mannopyranoside with strong concentration levels should always be stored in a temperature of 2 to 8 degree C and is used in research and manufacturing applications. Application: For Research or Further Manufacturing use only
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Antipain
RPI (Research Products International)Reversible cysteine and serine protease inhibitor of cathepsins A and B, papain and trypsin. Working concentration: 1-100uM.
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DEPC
RPI (Research Products International)Modification reagent for His and Tyr residues in proteins. Inhibitor of nucleases. DEPC treated water is used in handling of RNA in the laboratory to reduce the risk of RNA being degraded by RNases.
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4-Methylumbelliferyl Elaidate
RPI (Research Products International)Fluorescent substrate for Esterase. Purity: > 98%. 4-Methylumbelliferone < 50ppm. Soluble in 5% toluene.
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4-Methylumbelliferyl-α-D-mannopyranoside
RPI (Research Products International)Fluorescent substrate for α-D-Mannosidase. 4-Methylumbelliferone: <50ppm.
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1, 10-Phenanthroline
RPI (Research Products International)Forms a complex with ferrous ions. It can be used as an indicator in oxidation-reduction systems in fitrating ferrous salts. Material passes test for redox indicator and passes test for iron determination.
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X-GAL & IPTG, Ready to Use, Non-Toxic Solution
RPI (Research Products International)Stable solution of X-GAL and IPTG. Does not contain DMSO or DMF. Solution concentration is 10 mg/ml X-Gal and 8 mg/ml IPTG. Shelf Life: 1 Year
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IPTG
RPI (Research Products International)IPTG is combined with X-GAL to detect lac+ colonies or cells in a colorimetric assay. Distinguishes recombinants (white) from non- recombinants (blue) in cloning strategies using vectors such as Lambda-11, M13mp 18 and 19, pUC18 and 19, pUR222 containing the lacZ gene. Dioxane-Free Water…
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NPG
RPI (Research Products International)Spectrophotometric substrate for detection of B-D-Glucuronidase.
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4-Nitrophenyl-B-D-Glucopyranoside
RPI (Research Products International)Spectrophotometric substrate for detection of β-D-Glucosidase. Free PNP: <200ppm
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4-Methylumbelliferyl-a-D-Glucoside
RPI (Research Products International)Fluorescent substrate for 4-Methylumbelliferyl-α-D-Glucosidase. 4-Methylumbelliferone <50ppm.
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D-Luciferin, Free Acid
RPI (Research Products International)Used with firefly luciferase in bioluminescence for the determination of ATP.
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E-64
RPI (Research Products International)Irreversible cysteine protease inhibitor of calpain, cathepsin (B,H,L,S), papain and other cysteine proteases. Working concentration: 1-10uM.
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4-Methylumbelliferyl-α-D-galactoside
RPI (Research Products International)Fluorescent substrate for α-D-Galactosidase.
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MUG
RPI (Research Products International)Fluorescent substrate for β-D-Glucuronidase (GUS) encoded by the gusA gene isolated from E. coli. Fluorescence assay allows quantitation of GUS activity in protein extracts at peak excitation of 365 nm (UV) and a peak emission of 455 nm (blue).
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Protease Inhibitor Cocktail II, Bacterial
RPI (Research Products International)Lyophilized mixture of five protease inhibitors for use with bacterial cell extracts. Reconstitute with 1ml DMSO and 4ml water to obtain a 5ml solution. Will treat proteases extracted from 20g E.Coli. Store at -20°C Contains: AEBSF, EDTA, Bestatin, E-64, Pepstatin A
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4-Methylumbelliferyl-B-D-cellobiopyranoside
RPI (Research Products International)Fluorescent substrate for β-D-Cellobiopyranoside. 4-Methylumbelliferone < 50ppm.
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RPI Forskolin
RPI (Research Products International)The RPI forskolin is suitable for use in research facilities and chemical laboratories to conduct wide range of experiments. It is available in sturdy bottle that endures heavy and rigorous usage. Application: For Research or Further Manufacturing use only
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Phosphatase Inhibitor Cocktail I
RPI (Research Products International)Mixture of three phosphatase inhibitors for use with animal tissue. A431 and Jurkat cell extracts. Suitable for extracts containing detergents. Supplied in 1ml DMSO. Dilute 1:100 before using. Contains: (-)-p-Bromotetramisole oxalate Cantharidin Microcystin LR, Microcystis,…
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RPI Naphthol-AS-MX-phosphate
RPI (Research Products International)The RPI naphthol-AS-MX-phosphate is suitable for use in research facilities and chemical laboratories to conduct wide range of experiments. It is available in sturdy bottle that endures heavy and rigorous usage. Application: For Research or Further Manufacturing use only