MP Biomedicals Chemicals Enzymes and Inhibitors
MP Biomedicals
MP Biomedicals is a worldwide corporation manufacturing and distributing systems, kits and reagents for the life science research, medical device, biopharma manufacturing and diagnostics markets. Our extensive portfolio and custom services deliver the dependability that research and industrial communities rely on to worry less and discover more. From small scale research to large scale manufacturing, MP Biomedicals has been advancing life science projects for over 50 years.
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Choline Oxidase from Alcaligenes sp.
MP BiomedicalsCholine oxidase (EC 1.1.3.17) is an enzyme that catalyzes the chemical reaction Choline + O2 betaine aldehyde + H2O2. Thus, the two substrates of this enzyme are choline and O2, whereas its two products are betaine aldehyde and H2O2. This enzyme belongs to the family of oxidoreductases,…
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PHORBOL 12-MYRISTATE 13-ACETATE, 25 mg
MP BiomedicalsPMA activates Ca2+- ATPase and potentiates forskolin-induced cAMP formation. It has been shown to inhibit apoptosis induced by the Fas antigen, but PMA induces apoptosis in HL-60 promyelocytic leukemia cells.
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Catalase from Bovine Liver
MP BiomedicalsCatalase from bovine liver is a homotetrameric enzyme that is primarily located in peroxisomes. Activates the decomposition of hydrogen peroxide Natural antioxidant used to study roles of reactive oxygen species Catalyzes the decomposition of hydrogen peroxide into water and oxygen …
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Phosphoglucose Isomerase from Baker's Yeast
MP BiomedicalsPhosphoglucose Isomerase(PGI) is an enzyme crucial for the interconversion of D-glucose 6 phosphate and D-fructose 6-phosphate. Responsible for the second step of glycolysis Involved in glucogenesis Highly conserved in bacteria and eukaryotes Phosphoglucose Isomerase fuctions as an…
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Resveratrol
MP BiomedicalsPotent phenolic antioxidant found in grapes and red wine. Eicosanoid synthesis and platelet aggregation inhibitor. Estrogen receptor agonist. Chemopreventive. Specific inhibitor of cyclooxygenase-1 (COX-1). Anti-inflammatory. Ribonucleotide reductase and DNA synthesis. Arrests cell cycle at S/G2…
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Glucose Oxidase from Aspergillus niger
MP BiomedicalsGlucose oxidase is an FAD-containing glycoprotein. The enzyme is specific for β-D-glucose. O can be replaced by hydrogen acceptors such as 2,6-dichlorophenol indophenol. Glucose oxidase from Aspergillus niger is a dimer consisting of 2 equal subunits with a molecular mass of 80 kDa each. Each…
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Elastase from Porcine Pancreas
MP BiomedicalsElastase is an enzyme from the class of proteases (peptidases) that break down proteins. It hydrolyzes peptide bonds, especially those adjacent to neutral amino acids. Hydrolyses elastin Digests hemoglobin, casein, and fibrin One unit will solublize 1 mg of elastin in 20 minutes at…
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ß-Galactosidase from Echerichia coli
MP Biomedicalsβ-Galactosidase is useful for the structural investigation of carbohydrate, the determination of lactose (foodstuff analysis) and as an enzyme label for enzyme immunoassay. β-Galactosidase cleaves terminal galactose residues, which are β -1-->14 linked to a monosaccharide,…
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a-Amylase from Bacillus subtilis
MP BiomedicalsThis α-amylase product is a liquefying diastase supplied as a dry, free-flowing powder having high uniformity and excellent storage stability. The general procedure for the enzymatic liquefaction of starch is the same, regardless of the nature of the end product. The degree of conversion…
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Pectolyase Y-23 (A. niger)
MP BiomedicalsPectolyase Y-23 is a highly purified maceration enzyme from Aspergillus niger. It contains two types of pectinase such as endopolygalacturonase and endo-pectin lyase in high activity. Included is a maceration stimulating factor which stimulates tissue maceration by both pectinases. Unit Definition:…
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Cellulase from Aspergillis niger
MP BiomedicalsCellulase refers to a group of enzymes which, acting together, hydrolyze cellulose. The b-glucosidases, including cellobiose, are primarily active on the smaller molecular weight cellulose hydrolysates. During cellulose breakdown they are active on the dimers and oligomers of cellulose. …
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Papain from Papaya Latex
MP BiomedicalsPapain is a sulfhydryl protease from Carica papaya latex. Cleaves peptide bonds of basic amino acids, leucine, or glycine Hydrolyzes esters and amides One unit will hydrolyze 1.0 µmole of N-alpha-benzoyl-L-arginine ethyl ester (BAEE) per minute at 25°C and pH 6.2 Papain…
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Bilverdin Dihydrochloride
MP BiomedicalsBiliverdin Dihydrochloride is a bile pigment component. An oxidized metabolite of bilirubin caused when bilirubin reacts with reactive oxygen species. Biliverdin IX is composed of alpha, beta, gamma and delta isomers. Biliverdin dihydrochloride was found responsible for cytoprotection. It was…
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Dispase from Bacillus polymyxa
MP BiomedicalsDipase is an extremely stable microbial metalloprotease suitable for use in cell cultures. Dispase is suitable for tissue disaggregation and subcultivation procedures since it does not damage cell membranes. Dissolve the lyophilized enzyme in sterile water to 5 mg/mL. Further dilution with…
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Alkaline Phosphotase from E. coli
MP BiomedicalsAlkaline phosphatase is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. It is most effective in an alkaline environment. It is sometimes used synonymously as basic phosphatase. Alkaline phosphatase is used…
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Glucose-6-phosphate dehydrogenase
MP BiomedicalsGlucose-6-phosphate dehydrogenase (G-6-PDH) was used as a model to test the effect of seed protein fractions on enzyme protection during dehydration. G-6-PDH has been utilized in assays for nicotinamide adenine dinucleotide and tissue pyridine nucleotides.
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Trypsin from Beef Pancreas
MP BiomedicalsTrypsin consists of a single chain polypeptide of 223 amino acid residues. Member of the serine protease family Dissolves blood clots in its microbial form Treats inflammation in its pancreatic form Trypsin cleaves peptides on the C-terminal side of lysine and arginine residues.…
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ß-Glucosidase from Sweet Almonds
MP Biomedicalsβ-Glucosidase is a glucosidase enzyme. It is an exocellulase with specificity for a variety of β-D-glycoside substrates. β-Glucosidase is used in the synthesis of glucosides and fucosides with various potential applications in pharmaceutical, cosmetic and detergent industries,…
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Deoxyribonuclease I from Bovine Pancreas
MP BiomedicalsDeoxyribonuclease from beef pancreas, DNase I, was first crystallized by Kunitz. It is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding 5'-phosphate terminated polynucleotides with a free hydroxyl group on position 3'. The…
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Papain
MP BiomedicalsPapain is a sulfhydryl protease from Carica papaya latex. (A second protease, chymopapain, and a lysozyme have also been isolated from this same source.) Since native crystalline papain is quite unreactive until acted upon by mild reducing agents such as cysteine or cyanide, it may exist as a…
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ApopTag® Peroxidase
MP BiomedicalsApopTag® Peroxidase in situ detection kits label apoptotic cells in research samples by modifying DNA fragments utilizing terminal deoxynucleotidyl transferase (TdT) for detection of apoptotic cells by immuno-peroxidase detection of digoxigenin-labeled genomic DNA in thin sections of fixed…
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Hyaluronidase from Bovine Testes
MP BiomedicalsHyaluronidase is a glycoprotein containing 5% mannose and 2.17% glucosamine, it catalyzes the random hydrolysis of 1,4-linkages between 2-acetamido- 2-deoxy- b-D-glucose and D-glucose residues in hyaluronate. Hyaluronidase from bovine testes is a tetramer consisting of 4 equal subunits with a…
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4-(2-Aminoethyl)-Benzenesulfonyl Fluoride Hydrochloride
MP BiomedicalsAEBSF hydrochloride is a covalent binding serine protease inhibitor, belongs to the family of irreversible sulfonyl fluoride inhibitors that block trypsin and chymotrypsin-type enzymes. Similar in structure to the commonly used inhibitor PMSF, AEBSF offers better solubility in water, lower…
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Thrombin, bovine, 96.87 NIH U/mg dry weight, 5 x 2 KU
MP BiomedicalsThrombin is the final coagulation protease in regard to hemostasis, promoting both procoagulant and anticoagulant effects.
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Lysozyme from Chicken Eggwhite
MP BiomedicalsLysozyme (muramidase) hydrolyzes preferentially the β-1,4 glucosidic linkages between N-acetylmuramic acid and N-acetylglucosamine which occur in the mucopeptide cell wall structure of certain microorganisms, such as Micrococcus lysodeikticus. Extracts group specific antigens …
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Proteinase K Solution, Molecular Biology Grade
MP BiomedicalsFungus-Derived Proteinase K Purified Recombinant is prepared at 20 mg/mL in 10 mM Tris-HCl, 1 mM calcium acetate, pH 7.5 containing 50% glycerol. This Molecular Biology Grade Proteinase K solution is extremely effective on native proteins and can be used to rapidly inactivate endogenous nucleases…
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CELLULASE Y-C, 10 g
MP BiomedicalsCellulase is used for protoplast preparations. This enzyme shows a very high filter paper decomposing activity, as well as, appreciable xylanase and hemicellulase activity. It is an effective enzyme for use with pectolyase Y-23 for plant cell wall removal. It has been used in a study to assess the…
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a-Amylase from Porcine Pancreas
MP Biomedicalsα-Amylase isolated from porcine pancreas is a glycoprotein. It is a single polypeptide chain of ~475 residues containing two SH groups and four disulfide bridges and a tightly bound Ca2+ necessary for stability. Chloride ions are necessary for activity and stability. α-Amylase is…
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Iodoacetamide
MP BiomedicalsIodoacetamide is a thiol reagent; alkylating reagent for cysteine and histidine residues in proteins. In the alkylation reaction, it reacts with histidine (such as in RNase), methionine and sulfhydryl groups of many proteins. Iodoacetamide can react with low molecular weight thiol compounds such as…
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Collagenase from Clostridium histolyticum
MP BiomedicalsCollagenase products are prepared from culture supernatants of C. histolyticum. It is prepared to contain lower tryptic activity to limit damage to membrane proteins and receptors but with normal to above normal collagenase activity. Suggested for pacreatic islet isolation. Crude Collagenases…
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Phorbol-12-Myristate-13-Acetate, 1 Mg
MP BiomedicalsPMA activates Ca2+- ATPase and potentiates forskolin-induced cAMP formation. It has been shown to inhibit apoptosis induced by the Fas antigen, but PMA induces apoptosis in HL-60 promyelocytic leukemia cells.