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Enzymes & Inhibitors, MP Biomedicals

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MP Biomedicals Enzymes & Inhibitors

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  • Pancreatin 8X from Porcine Pancreas

    MP Biomedicals

    Pancreatin is a mixture of several digestive enzymes produced by the exocrine cells of the porcine pancreas and delivered to the small intestine for the hydrolysis of complex nutrients. It is a broad-spectrum protease composed of amylase, trypsin, lipase, ribonuclease and protease. Pancreatin…

  • Glucose Oxidase from Aspergillus niger

    MP Biomedicals

    Glucose oxidase is an FAD-containing glycoprotein. The enzyme is specific for β-D-glucose. O can be replaced by hydrogen acceptors such as 2,6-dichlorophenol indophenol. Glucose oxidase from Aspergillus niger is a dimer consisting of 2 equal subunits with a molecular mass of 80 kDa each. Each…

  • p-Nitrophenyl Phopshate Disodium Salt Hexahydrate

    MP Biomedicals

    p-nitrophenol is suitable for use as a substrate for alkaline and acid phosphatase. p nitrophenol is the substrate of choice for use with alkaline phosphatase in ELISA procedures. This substrate produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405…

  • Deoxyribonuclease I from Bovine Pancreas

    MP Biomedicals

    Deoxyribonuclease from beef pancreas, DNase I, was first crystallized by Kunitz. It is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding 5'-phosphate terminated polynucleotides with a free hydroxyl group on position 3'. The…

  • Elastase from Porcine Pancreas

    MP Biomedicals

    Elastase is an enzyme from the class of proteases (peptidases) that break down proteins. It hydrolyzes peptide bonds, especially those adjacent to neutral amino acids. Hydrolyses elastin Digests hemoglobin, casein, and fibrin One unit will solublize 1 mg of elastin in 20 minutes at…

  • Hemin Chloride from Porcine

    MP Biomedicals

    Hemin is an iron-containing porphyrin. It is a Protoporphyrin IX containing a ferric iron ion (Heme B) with a chloride ligand. Hemin is used in the management of porphyria attacks, particularly in acute intermittent porphyria. It may be used to study the expression and repression of heme…

  • Lysozyme from Chicken Eggwhite

    MP Biomedicals

    Lysozyme (muramidase) hydrolyzes preferentially the β-1,4 glucosidic linkages between N-acetylmuramic acid and N-acetylglucosamine which occur in the mucopeptide cell wall structure of certain microorganisms, such as Micrococcus lysodeikticus. Extracts group specific antigens …

  • Pectinase from Aspergillus niger

    MP Biomedicals

    Pectinase is a macerating enzyme comprised of pectintranseliminase, polygalacturonase, pectinesterase, xylanase, hemicellulases and cellulases. Pectinase is used in plant protoplast preparation to digest cell wall prior to organelle isolation. Pectinase causes random hydrolysis of…

  • Pectin from Apple

    MP Biomedicals

    Pectin is a heterosaccharide, component of terrestrial plant cell walls. Pectin is used as a substrate to identify, differentiate and characterized pectinase(s). It is also used to study its degradation by pectinolytic bacteria.

  • Lysozyme from Chicken Eggwhite

    MP Biomedicals

    Lysozyme (muramidase) hydrolyzes preferentially the β-1,4 glucosidic linkages between N-acetylmuramic acid and N-acetylglucosamine which occur in the mucopeptide cell wall structure of certain microorganisms, such as Micrococcus lysodeikticus. Extracts group specific antigens …

  • Diethyl Pyrocarbonate

    MP Biomedicals

    Clear, Colorless Liquid Synonyms: Diethyl Oxydiformate, Dicarbonic Acid Diethyl Ester, DEPC, Ethoxyformic acid anhydride, Diethyl dicarbonate CAS Number: 1609-47-8 Molecular Formula: C6H10O5 Molecular Weight: 162.1 Beilstein…

  • Probenecid

    MP Biomedicals

    Probenecid is a uricosuric agent, which increases uric acid removal in the urine. It also reduces the renal tubular excretion of many other drugs and increases their plasma concentration. Probenecid is useful as an inhibitor of the organic anion transporter. Probenecid is an inhibitor of…

  • Manganese Chloride Tetrahydrate

    MP Biomedicals

    Manganese chloride occurs in nature as the mineral scacchite. It is used in industrial applications such as dyeing and disinfecting, the purification of natural gas, and as a drying agent for linseed oil. In biochemistry, manganese is a cofactor in restriction enzymes such as BamH I and EcoR V. …

  • Thiamine Hydrochloride

    MP Biomedicals

    Thiamine (Vitamin B1) is one of the essential vitamins and is required for carbohydrate metabolism. Thiamine hydrochloride is used as a food-additive to add a brothy/meaty flavor to gravies or soups. It is also used as food supplement. Used in fluorometric determination of mercury.

  • Hydroxylamine Hydrochloride

    MP Biomedicals

    MAO inhibitor; inhibits platelet aggregation.

  • 4-(2-Aminoethyl)-Benzenesulfonyl Fluoride Hydrochloride

    MP Biomedicals

    AEBSF hydrochloride is a covalent binding serine protease inhibitor, belongs to the family of irreversible sulfonyl fluoride inhibitors that block trypsin and chymotrypsin-type enzymes. Similar in structure to the commonly used inhibitor PMSF, AEBSF offers better solubility in water, lower…

  • Hyaluronidase from Ovine Testes, >=1,000 I.U./mg Material

    MP Biomedicals

    Hyaluronidase is a glycoprotein containing 5% mannose and 2.17% glucosamine, it catalyzes the random hydrolysis of 1,4-linkages between 2-acetamido- 2-deoxy- β-D-glucose and D-glucose residues in hyaluronate. Hyaluronidase from bovine testes is a tetramer consisting of 4 equal subunits with a…

  • Cathepsin G from Human Neutrophils

    MP Biomedicals

    Cathepsin G is a serine protease that degrades collagen and proteoglycans. It has been implicated in connective tissue-related disorders. Cathepsin G from human leukocytes is a glycoprotein containing approximately 1% carbohydrate. It is considered to be a major constituent of human neutophil…

  • Creatine Amidohydrolase from Pseudomonas sp.

    MP Biomedicals

    This enzyme is useful for enzymatic determination of creatinine when coupled with creatine amidinohydrolase, sarcosine dehydrogenase or sarcosine oxidase and formaldehyde dehydrogenase in clinical analysis. Creatininase from Pseudomonas sp. is a homohexameric enzyme with a molecular mass of 28.4…

  • Naproxen

    MP Biomedicals

    Pharmacological agent that reportedly may show activity through ability to inhibit prostaglandin biosynthesis. Non-selective cyclooxygenase (COX-1 and COX-2) inhibitor. Naproxen has been demonstrated to block Aβ fibril growth and form a complex with copper (II).

  • ApopTag® Peroxidase

    MP Biomedicals

    ApopTag® Peroxidase in situ detection kits label apoptotic cells in research samples by modifying DNA fragments utilizing terminal deoxynucleotidyl transferase (TdT) for detection of apoptotic cells by immuno-peroxidase detection of digoxigenin-labeled genomic DNA in thin sections of fixed…

  • Collagenase Type 3 from Clostridium histolyticum

    MP Biomedicals

    Collagenases degrade native helical collagen fibrils. The substrate, collagen, is the major fibrous component of animal extracellular connective tissue: skin, tendon, blood vessels, bone, etc. True collagenase may cleave simultaneously across all three chains or attack a single strand. The enzyme…

  • Macerozyme from Rhizopus sp.

    MP Biomedicals

    Petctinase is an enzyme from Rhizopus sp. that is used in plant protoplast preparation to digest cell wall prior to organelle isolation. It has been used to digest the cell wall of Arabidopsis root cells to study peroxin 16 in peroxisomes and endoplasmic reticulum. Pectinases are used to study…

  • Deoxyribonuclease I from Bovine Pancreas

    MP Biomedicals

    Deoxyribonuclease from beef pancreas, DNase I, was first crystallized by Kunitz. It is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding 5'-phosphate terminated polynucleotides with a free hydroxyl group on position 3'. The…

  • a-Amylase from Porcine Pancreas

    MP Biomedicals

    α-Amylase isolated from porcine pancreas is a glycoprotein. It is a single polypeptide chain of ~475 residues containing two SH groups and four disulfide bridges and a tightly bound Ca2+ necessary for stability. Chloride ions are necessary for activity and stability. α-Amylase is…

  • D-Val-Leu-Lys-7-Amino-4-Methylcoumarin

    MP Biomedicals

    The AMC fluorogenic substrates are widely used for routine assays of peptidases. A short peptide sequence is conjugated to 7-AMINO-4-METHYLCOUMARIN, which is released upon cleavage by peptidase. The release of 7-AMINO-4-METHYLCOUMARIN is then measured spectrophotometrically. The excitation; 380 nm…

  • Collagenase Type IV from Clostridium histolyticum

    MP Biomedicals

    Collagenases degrade native helical collagen fibrils. It has an important role in connective tissue metabolism and is produced by specific cells involved in repairs and remodelling processes. Collagenase is used for tissue dissociation combined with other enzymes such as elastase, trypsin,…

  • a-Amylase from Bacillus subtilis

    MP Biomedicals

    This α-amylase product is a liquefying diastase supplied as a dry, free-flowing powder having high uniformity and excellent storage stability. The general procedure for the enzymatic liquefaction of starch is the same, regardless of the nature of the end product. The degree of conversion…

  • Acid Phosphatase from Wheat Germ

    MP Biomedicals

    Acid phosphatase (APase) non-specifically catalyzes the hydrolysis of monoesters and anhydrides of phosphoric acid to produce inorganic phosphate. It is used to study the production, transport, and recycling of phosphate and the metabolic and energy transduction processes of the cell. Acid…

  • L-Glutamic Dehydrogenase from Bovine Liver

    MP Biomedicals

    L-glutamic dehydrogenase is a pyridine nucelotide enzyme which catalyzes the reversible oxidative deamination of L-glutamate to α-keto-glutarate and ammonia. L-glutamic dehydrogenase plays a unique role in mammalian metabolism. The reverse reaction catalyzed by this enzyme is the only pathway…

  • Catalase from Bovine Liver

    MP Biomedicals

    Catalase from bovine liver is a homotetrameric enzyme that is primarily located in peroxisomes. Activates the decomposition of hydrogen peroxide Natural antioxidant used to study roles of reactive oxygen species Catalyzes the decomposition of hydrogen peroxide into water and oxygen …

  • Protease Inhibitor Cocktail Kit

    MP Biomedicals

    Protease Inhibitor Cocktail Kit is a broad spectrum protease inhibitor cocktail which covers both endopeptidases and exopeptidases, non-specific and specific. The cocktail consists of four major protease inhibitors: AEBSF, Sodium EDTA, Leupeptin and Pepstatin A. Protease Inhibitor Cocktail Kit…

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