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Enzymes & Inhibitors, MP Biomedicals

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MP Biomedicals Enzymes & Inhibitors

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  • Protease Inhibitor Cocktail Kit

    MP Biomedicals

    Protease Inhibitor Cocktail Kit is a broad spectrum protease inhibitor cocktail which covers both endopeptidases and exopeptidases, non-specific and specific. The cocktail consists of four major protease inhibitors: AEBSF, Sodium EDTA, Leupeptin and Pepstatin A. Protease Inhibitor Cocktail Kit…

  • 5-Bromo-2'-Deoxyuridine

    MP Biomedicals

    5-Bromo-2’-deoxyuridine is a thymidine analog and useful for the study of DNA synthesis where it is incorporated into DNA in place of thymidine. Measure DNA synthesis Label dividing cells Study cell signaling and processes that induce cell proliferation Used as a mutagen in…

  • Spermidine, Trihydrochloride

    MP Biomedicals

    Spermidine is a biogenic polyamine formed from putrescine. Essential in both normal and neoplastic tissue growth Plays an important role in the regulation of cellular proliferation and differentiation Forms stable compounts with nucleic acids Inhibits neuronal nitric oxide synthase…

  • a-Chymotripsin from Bovine Pancreas

    MP Biomedicals

    A serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met, Leu) on the carboxyl end of the bond. Produced from 3x Crystallized chymotrypsinogen Used to treat pancreatic insufficiency Used in traumatology Chymotrypsin…

  • Acetyl Coenzyme A Trilithium Salt Trihydrate

    MP Biomedicals

    Acetyl-CoA is produced via beta-oxidation of fatty acids, via the metabolism of carbohydrates - glucose 6-phosphate to pyruvate to acetyl-CoA and via the catabolism of amino acids. Acetyl-CoA has a number of metabolic opportunities. It is metabolized in the tricarboxylic acid cycle to produce…

  • Phenylmethylsulfonyl Fluoride

    MP Biomedicals

    Phenylmethylsulfonyl fluoride is an irreversible inhibitor of serine proteases - inhibits trypsin, chymotrypsin and protease. Inhibits mammalian acetyl cholinesterase, but not those from fish sources. Administration of PMSF produces analgesia unrelated to its anticholinesterase effect, and…

  • Pepstatin A

    MP Biomedicals

    Pepstatin A is a reversible inhibitor of aspartic proteases, Inhibitor for pepsin, renin, cathepsin D, cathepsin G, and other acid proteases. It does not inhibit thiol proteases, neutral proteases or serine proteases. Pepstatin forms a 1:1 complex with acid proteases (carboxyl proteases). …

  • 4-(2-Aminoethyl)-Benzenesulfonyl Fluoride Hydrochloride

    MP Biomedicals

    AEBSF hydrochloride is a covalent binding serine protease inhibitor, belongs to the family of irreversible sulfonyl fluoride inhibitors that block trypsin and chymotrypsin-type enzymes. Similar in structure to the commonly used inhibitor PMSF, AEBSF offers better solubility in water, lower…

  • Leupeptin Hemisulfate

    MP Biomedicals

    Leupeptin is a modified tripeptide reversible protease inhibitor of trypsin-like proteases and some cysteine proteases including endoproteinase Lys-C, kallikrein, papain, thrombin, cathepsin B, cathepsins H and L, trypsin, calpain, trypsin8 and plasmin. Effective concentration is typically 10-100…

  • E-64 from Synthetic

    MP Biomedicals

    E-64 is an inhibitor for thiol protease, papain, actinidin, ananain, bromelain, calpain and cathepsin B. It is an irreversible cysteine protease inhibitor that has no action on cysteine residues in other plants and does not react with low molecular weight thiols such as 2-mercaptoethanol. Specific…

  • Diethyl Pyrocarbonate

    MP Biomedicals

    Clear, Colorless Liquid Synonyms: Diethyl Oxydiformate, Dicarbonic Acid Diethyl Ester, DEPC, Ethoxyformic acid anhydride, Diethyl dicarbonate CAS Number: 1609-47-8 Molecular Formula: C6H10O5 Molecular Weight: 162.1 Beilstein…

  • Hydroxylamine Hydrochloride

    MP Biomedicals

    MAO inhibitor; inhibits platelet aggregation.

  • Phosphodiesterase I from Crotalus adamanteus

    MP Biomedicals

    Phosphodiesterase I successively hydrolyzes 5'-mononucleotides from 3'-hydroxy-terminated ribo- and deoxyribo-oligonucleotides. ADP-ribosylated proteins are cleaved at the pyrophosphate linkages by venom phosphodiesterases to yield phosphoribosyl-AMP. Enzyme that is used to break…

  • Hyaluronidase from Bovine Testes

    MP Biomedicals

    Hyaluronidase is a glycoprotein containing 5% mannose and 2.17% glucosamine, it catalyzes the random hydrolysis of 1,4-linkages between 2-acetamido- 2-deoxy- b-D-glucose and D-glucose residues in hyaluronate. Hyaluronidase from bovine testes is a tetramer consisting of 4 equal subunits with a…

  • Iodoacetamide

    MP Biomedicals

    Iodoacetamide is a thiol reagent; alkylating reagent for cysteine and histidine residues in proteins. In the alkylation reaction, it reacts with histidine (such as in RNase), methionine and sulfhydryl groups of many proteins. Iodoacetamide can react with low molecular weight thiol compounds such as…

  • Catalase from Bovine Liver

    MP Biomedicals

    Catalase from bovine liver is a homotetrameric enzyme that is primarily located in peroxisomes. Activates the decomposition of hydrogen peroxide Natural antioxidant used to study roles of reactive oxygen species Catalyzes the decomposition of hydrogen peroxide into water and oxygen …

  • Catalase from Aspergillus niger

    MP Biomedicals

    Catalase, Fungal suspension from from Aspergillus niger is long-acting, extremely stable form of catalase that is active over a wide pH range. Composed of four protein subunits Each subunit contains a heme group bound to its active site Catalase is used for the removal of peroxides,…

  • Lysozyme from Chicken Eggwhite

    MP Biomedicals

    Lysozyme (muramidase) hydrolyzes preferentially the β-1,4 glucosidic linkages between N-acetylmuramic acid and N-acetylglucosamine which occur in the mucopeptide cell wall structure of certain microorganisms, such as Micrococcus lysodeikticus. Extracts group specific antigens …

  • ß-Mercaptoethylamine Hydrochloride

    MP Biomedicals

    β-Mercaptoethylamine is a useful antioxidant .It is experimentally used as a radioprotective agent and It is also used to produce acute and chronic duodenal ulcers in rats and as an antidote to acetaminophen. β-Mercaptoethylamine derived from cysteine degradation is the simplest stable…

  • Lipase from Porcine Pancreas

    MP Biomedicals

    Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel. Subclass of esterases Catalyzes hydrolysis of emulsified esters of glycerol and long chain fatty acids One unit will liberate 100 µ moles fatty acid per hour…

  • Deoxyribonuclease I from Bovine Pancreas

    MP Biomedicals

    Deoxyribonuclease from beef pancreas, DNase I, was first crystallized by Kunitz. It is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding 5'-phosphate terminated polynucleotides with a free hydroxyl group on position 3'. The…

  • p-Nitrophenyl Phopshate Disodium Salt Hexahydrate

    MP Biomedicals

    p-nitrophenol is suitable for use as a substrate for alkaline and acid phosphatase. p nitrophenol is the substrate of choice for use with alkaline phosphatase in ELISA procedures. This substrate produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405…

  • Lactase from Aspergillus oryzae

    MP Biomedicals

    Lactase is an enzyme preparation produced by Aspergillus oryzae fermentation. Hydrolyzes Lactose Produces β-D-Galactose and a-D-Glucose One unit will hydrolyze 1.0 µmole of o-nitrophenyl-beta-D-galactoside to o-nitrophenol per minute at pH 4.5 and 30°C …

  • o-Dianisidine

    MP Biomedicals

    o-Dianisidine is a peroxidase substrate suitable for use in ELISA procedures. o-Dianisidine is used for the detection of Au, Co, Cu, SCN-, V; determination of Au, NO2-, Ce(IV) (spectrophotometrically). Ortho Dianisidine may be used in a novel automated method, used to measure the total…

  • Catalase from Beef Liver

    MP Biomedicals

    Catalase is an oxidoreductase that catalyzes the conversion of hydrogen peroxide to water and oxygen. Activates the decomposition of hydrogen peroxide Natural antioxidant Used to study roles of reactive oxygen species in gene expression and apoptosis Hydrogen peroxide is a harmful…

  • Alcohol Oxidase from Pichea Pastoris

    MP Biomedicals

    ≥10 u/mg Protein, Reddish Brown Frozen Liquid Alcohol oxidase has been detected in several strains of yeast,such as Candida, Pichia, and Hansenula,that utilize methanol as a sole carbon and energy source. Some of these enzymes have been isolated and partially characterized. Alcohol oxidase…

  • Naphthol AS-D Chloroacetate

    MP Biomedicals

    Naphthol AS-D chloroacetate is a substrate for histochemical localization of esterases.

  • Trypsin Inhibitor from Soybean

    MP Biomedicals

    Trypsin inhibitor from soybeans is a monomeric protein containing 181 amino acid residues in a single polypeptide chain crosslinked by two disulfide bridges. Reversible serine protease inhibitor Inhibits factor Xa, trypsin, chymotrypsin, kallikrein and plasmin One unit will inhibit one…

  • Papain

    MP Biomedicals

    Papain is a sulfhydryl protease from Carica papaya latex. (A second protease, chymopapain, and a lysozyme have also been isolated from this same source.) Since native crystalline papain is quite unreactive until acted upon by mild reducing agents such as cysteine or cyanide, it may exist as a…

  • Lysozyme from Chicken Eggwhite

    MP Biomedicals

    Lysozyme (muramidase) hydrolyzes preferentially the β-1,4 glucosidic linkages between N-acetylmuramic acid and N-acetylglucosamine which occur in the mucopeptide cell wall structure of certain microorganisms, such as Micrococcus lysodeikticus. Extracts group specific antigens …

  • ß-Glucosidase from Sweet Almonds

    MP Biomedicals

    Beta-glucosidase is a glucosidase enzyme that acts upon β1->4 bonds linking two glucose or glucose-substituted molecules (i.e., the disaccharide cellobiose). Exocellulase with a specificity for a variety of beta-D-glycoside substrates Catalyzes the hydrolysis of terminal…

  • a-Amylase from Bacillus subtilis

    MP Biomedicals

    This α-amylase product is a liquefying diastase supplied as a dry, free-flowing powder having high uniformity and excellent storage stability. The general procedure for the enzymatic liquefaction of starch is the same, regardless of the nature of the end product. The degree of conversion…

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