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Enzymes & Inhibitors, MP Biomedicals

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MP Biomedicals Enzymes & Inhibitors

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  • Pancreatin 8X from Porcine Pancreas

    MP Biomedicals

    Pancreatin is a mixture of several digestive enzymes produced by the exocrine cells of the porcine pancreas and delivered to the small intestine for the hydrolysis of complex nutrients. It is a broad-spectrum protease composed of amylase, trypsin, lipase, ribonuclease and protease. Pancreatin…

  • Lysozyme from Chicken Eggwhite

    MP Biomedicals

    Lysozyme (muramidase) hydrolyzes preferentially the β-1,4 glucosidic linkages between N-acetylmuramic acid and N-acetylglucosamine which occur in the mucopeptide cell wall structure of certain microorganisms, such as Micrococcus lysodeikticus. Extracts group specific antigens …

  • ApopTag® Peroxidase

    MP Biomedicals

    ApopTag® Peroxidase in situ detection kits label apoptotic cells in research samples by modifying DNA fragments utilizing terminal deoxynucleotidyl transferase (TdT) for detection of apoptotic cells by immuno-peroxidase detection of digoxigenin-labeled genomic DNA in thin sections of fixed…

  • Deoxyribonuclease I from Bovine Pancreas

    MP Biomedicals

    Deoxyribonuclease from beef pancreas, DNase I, was first crystallized by Kunitz. It is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding 5'-phosphate terminated polynucleotides with a free hydroxyl group on position 3'. The…

  • Collagenase Type 3 from Clostridium histolyticum

    MP Biomedicals

    Collagenases degrade native helical collagen fibrils. The substrate, collagen, is the major fibrous component of animal extracellular connective tissue: skin, tendon, blood vessels, bone, etc. True collagenase may cleave simultaneously across all three chains or attack a single strand. The enzyme…

  • Macerozyme from Rhizopus sp.

    MP Biomedicals

    Petctinase is an enzyme from Rhizopus sp. that is used in plant protoplast preparation to digest cell wall prior to organelle isolation. It has been used to digest the cell wall of Arabidopsis root cells to study peroxin 16 in peroxisomes and endoplasmic reticulum. Pectinases are used to study…

  • Deoxyribonuclease I from Bovine Pancreas

    MP Biomedicals

    Deoxyribonuclease from beef pancreas, DNase I, was first crystallized by Kunitz. It is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding 5'-phosphate terminated polynucleotides with a free hydroxyl group on position 3'. The…

  • Hyaluronidase from Ovine Testes, >=1,000 I.U./mg Material

    MP Biomedicals

    Hyaluronidase is a glycoprotein containing 5% mannose and 2.17% glucosamine, it catalyzes the random hydrolysis of 1,4-linkages between 2-acetamido- 2-deoxy- β-D-glucose and D-glucose residues in hyaluronate. Hyaluronidase from bovine testes is a tetramer consisting of 4 equal subunits with a…

  • a-Amylase from Porcine Pancreas

    MP Biomedicals

    α-Amylase isolated from porcine pancreas is a glycoprotein. It is a single polypeptide chain of ~475 residues containing two SH groups and four disulfide bridges and a tightly bound Ca2+ necessary for stability. Chloride ions are necessary for activity and stability. α-Amylase is…

  • Lysozyme from Chicken Eggwhite

    MP Biomedicals

    Lysozyme (muramidase) hydrolyzes preferentially the β-1,4 glucosidic linkages between N-acetylmuramic acid and N-acetylglucosamine which occur in the mucopeptide cell wall structure of certain microorganisms, such as Micrococcus lysodeikticus. Extracts group specific antigens …

  • D-Val-Leu-Lys-7-Amino-4-Methylcoumarin

    MP Biomedicals

    The AMC fluorogenic substrates are widely used for routine assays of peptidases. A short peptide sequence is conjugated to 7-AMINO-4-METHYLCOUMARIN, which is released upon cleavage by peptidase. The release of 7-AMINO-4-METHYLCOUMARIN is then measured spectrophotometrically. The excitation; 380 nm…

  • p-Nitrophenyl Phopshate Disodium Salt Hexahydrate

    MP Biomedicals

    p-nitrophenol is suitable for use as a substrate for alkaline and acid phosphatase. p nitrophenol is the substrate of choice for use with alkaline phosphatase in ELISA procedures. This substrate produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405…

  • Collagenase Type IV from Clostridium histolyticum

    MP Biomedicals

    Collagenases degrade native helical collagen fibrils. It has an important role in connective tissue metabolism and is produced by specific cells involved in repairs and remodelling processes. Collagenase is used for tissue dissociation combined with other enzymes such as elastase, trypsin,…

  • Elastase from Porcine Pancreas

    MP Biomedicals

    Elastase is an enzyme from the class of proteases (peptidases) that break down proteins. It hydrolyzes peptide bonds, especially those adjacent to neutral amino acids. Hydrolyses elastin Digests hemoglobin, casein, and fibrin One unit will solublize 1 mg of elastin in 20 minutes at…

  • a-Amylase from Bacillus subtilis

    MP Biomedicals

    This α-amylase product is a liquefying diastase supplied as a dry, free-flowing powder having high uniformity and excellent storage stability. The general procedure for the enzymatic liquefaction of starch is the same, regardless of the nature of the end product. The degree of conversion…

  • Acid Phosphatase from Wheat Germ

    MP Biomedicals

    Acid phosphatase (APase) non-specifically catalyzes the hydrolysis of monoesters and anhydrides of phosphoric acid to produce inorganic phosphate. It is used to study the production, transport, and recycling of phosphate and the metabolic and energy transduction processes of the cell. Acid…

  • L-Glutamic Dehydrogenase from Bovine Liver

    MP Biomedicals

    L-glutamic dehydrogenase is a pyridine nucelotide enzyme which catalyzes the reversible oxidative deamination of L-glutamate to α-keto-glutarate and ammonia. L-glutamic dehydrogenase plays a unique role in mammalian metabolism. The reverse reaction catalyzed by this enzyme is the only pathway…

  • Pectin from Apple

    MP Biomedicals

    Pectin is a heterosaccharide, component of terrestrial plant cell walls. Pectin is used as a substrate to identify, differentiate and characterized pectinase(s). It is also used to study its degradation by pectinolytic bacteria.

  • Catalase from Bovine Liver

    MP Biomedicals

    Catalase from bovine liver is a homotetrameric enzyme that is primarily located in peroxisomes. Activates the decomposition of hydrogen peroxide Natural antioxidant used to study roles of reactive oxygen species Catalyzes the decomposition of hydrogen peroxide into water and oxygen …

  • Protease Inhibitor Cocktail Kit

    MP Biomedicals

    Protease Inhibitor Cocktail Kit is a broad spectrum protease inhibitor cocktail which covers both endopeptidases and exopeptidases, non-specific and specific. The cocktail consists of four major protease inhibitors: AEBSF, Sodium EDTA, Leupeptin and Pepstatin A. Protease Inhibitor Cocktail Kit…

  • Acetoacetyl Coenzyme A Trisodium Salt Tetrahydrate

    MP Biomedicals

    Acetoacetyl coenzyme A (Acetoacetyl-CoA) may be used as a substrate for a number of enzymes including; acetoacetyl-CoA thiolase (EC 2.3.1) which produces acetyl-CoA in reverse condensation reaction and (3-Hydroxy-3-methylglutaryl coenzyme A (CoA) synthase (HMGCS)) which produces…

  • Esculin Monohydrate

    MP Biomedicals

    Esculin Monohydrate protects cells against DNA damage and reduces ROS levels. It is also a carcinogenesis inhibitor and displays vitamin P activity. Esculin is a coumarin glycoside that demonstrates antioxidant activitites and protection against chemically-induced carcinogenesis. Esculin is…

  • Catalase from Beef Liver

    MP Biomedicals

    Catalase is an oxidoreductase that catalyzes the conversion of hydrogen peroxide to water and oxygen. Activates the decomposition of hydrogen peroxide Natural antioxidant Used to study roles of reactive oxygen species in gene expression and apoptosis Hydrogen peroxide is a harmful…

  • Pepsin from Porcine

    MP Biomedicals

    Pepsin, an acid protease, contains a proteolytic enzyme. Pepsin contains the "cathepsin" component which has milk curdling activity. It has a broad range of substrate activity and demonstrates an esterase acitivity. It generally attacks peptide bonds. Pepsin is a peptidase used to…

  • Collagenase Type II from Clostridium histolyticum

    MP Biomedicals

    Collagenases degrade native helical collagen fibrils. Plays an important role in connective tissue metabolism Produced by specific cells involved in repairs and remodeling processes Type II enzyme that contains greater clostripain activity Collagenase is used for collagen…

  • 5-Bromo-2'-Deoxyuridine

    MP Biomedicals

    5-Bromo-2’-deoxyuridine is a thymidine analog and useful for the study of DNA synthesis where it is incorporated into DNA in place of thymidine. Measure DNA synthesis Label dividing cells Study cell signaling and processes that induce cell proliferation Used as a mutagen in…

  • Diastase from Malt

    MP Biomedicals

    1X N.F. Stable, 60 mesh powder. 1 gram will digest 50 gram starch in 30 minutes.

  • Spermidine, Trihydrochloride

    MP Biomedicals

    Spermidine is a biogenic polyamine formed from putrescine. Essential in both normal and neoplastic tissue growth Plays an important role in the regulation of cellular proliferation and differentiation Forms stable compounts with nucleic acids Inhibits neuronal nitric oxide synthase…

  • Acetyl Coenzyme A Trilithium Salt Trihydrate

    MP Biomedicals

    Acetyl-CoA is produced via beta-oxidation of fatty acids, via the metabolism of carbohydrates - glucose 6-phosphate to pyruvate to acetyl-CoA and via the catabolism of amino acids. Acetyl-CoA has a number of metabolic opportunities. It is metabolized in the tricarboxylic acid cycle to produce…

  • a-Chymotripsin from Bovine Pancreas

    MP Biomedicals

    A serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met, Leu) on the carboxyl end of the bond. Produced from 3x Crystallized chymotrypsinogen Used to treat pancreatic insufficiency Used in traumatology Chymotrypsin…

  • Phenylmethylsulfonyl Fluoride

    MP Biomedicals

    Phenylmethylsulfonyl fluoride is an irreversible inhibitor of serine proteases - inhibits trypsin, chymotrypsin and protease. Inhibits mammalian acetyl cholinesterase, but not those from fish sources. Administration of PMSF produces analgesia unrelated to its anticholinesterase effect, and…

  • Pepstatin A

    MP Biomedicals

    Pepstatin A is a reversible inhibitor of aspartic proteases, Inhibitor for pepsin, renin, cathepsin D, cathepsin G, and other acid proteases. It does not inhibit thiol proteases, neutral proteases or serine proteases. Pepstatin forms a 1:1 complex with acid proteases (carboxyl proteases). …

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