Papain is a sulfhydryl protease from Carica papaya latex. (A second protease, chymopapain, and a lysozyme have also been isolated from this same source.) Since native crystalline papain is quite unreactive until acted upon by mild reducing agents such as cysteine or cyanide, it may exist as a…

Biliverdin Dihydrochloride is a bile pigment component. An oxidized metabolite of bilirubin caused when bilirubin reacts with reactive oxygen species. Biliverdin IX is composed of alpha, beta, gamma and delta isomers. Biliverdin dihydrochloride was found responsible for cytoprotection. It was…

α-Thrombin from Bovine Plasma is highly specific serine protease. Thrombin is the final coagulation protease in regard to hemostasis, promoting both procoagulant and anticoagulant effects. Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible…

Pepsin, an acid protease, contains a proteolytic enzyme. Pepsin contains the "cathepsin" component which has milk curdling activity. It has a broad range of substrate activity and demonstrates an esterase acitivity. It generally attacks peptide bonds. Pepsin is a peptidase used to…

Alcohol oxidase has been detected in several strains of yeast, such as Candida, Pichia, and Hansenula, that utilize methanol as a sole carbon and energy source. Some of these enzymes have been isolated and partially characterized. Alcohol oxidase plays a major role in the metabolism of methanol…

Collagenases degrade native helical collagen fibrils. The substrate, collagen, is the major fibrous component of animal extracellular connective tissue: skin, tendon, blood vessels, bone, etc. True collagenase may cleave simultaneously across all three chains or attack a single strand. The enzyme…

Fungus-Derived Proteinase K Purified Recombinant is prepared at 20 mg/mL in 10 mM Tris-HCl, 1 mM calcium acetate, pH 7.5 containing 50% glycerol. This Molecular Biology Grade Proteinase K solution is extremely effective on native proteins and can be used to rapidly inactivate endogenous nucleases…

Cell permeable mycotoxin. Actin polymerization inhibitor. Binds to the barbed end of actin, reversibly inhibiting the elongation and shortening of actin filaments. Induces nuclear extrusion. By disrupting actin polymerization, blocks diverse cellular functions, including cell division, migration,…

Collagenases degrade native helical collagen fibrils. Plays an important role in connective tissue metabolism Produced by specific cells involved in repairs and remodeling processes Type II enzyme that contains greater clostripain activity Collagenase is used for collagen…

2,2'-Azino-bis-(3-ethylbenzthiazoline-6-sulfonic Acid) Solution is oxidized to a radical action showing absorption maxima at 820 nm, 734 nm, 650 nm and 405 nm, in the presence of hydrogen peroxide and HRP. The latter frequency demonstrates a significant molar extinction coefficient and is…

Pectolyase Y-23 is a highly purified maceration enzyme from Aspergillus niger. It contains two types of pectinase such as endopolygalacturonase and endo-pectin lyase in high activity. Included is a maceration stimulating factor which stimulates tissue maceration by both pectinases. Unit Definition:…

ApopTag® Peroxidase in situ detection kits label apoptotic cells in research samples by modifying DNA fragments utilizing terminal deoxynucleotidyl transferase (TdT) for detection of apoptotic cells by immuno-peroxidase detection of digoxigenin-labeled genomic DNA in thin sections of fixed…

Acetyl-CoA is produced via beta-oxidation of fatty acids, via the metabolism of carbohydrates - glucose 6-phosphate to pyruvate to acetyl-CoA and via the catabolism of amino acids. Acetyl-CoA has a number of metabolic opportunities. It is metabolized in the tricarboxylic acid cycle to produce…

5-Bromo-2’-deoxyuridine is a thymidine analog and useful for the study of DNA synthesis where it is incorporated into DNA in place of thymidine. Measure DNA synthesis Label dividing cells Study cell signaling and processes that induce cell proliferation Used as a mutagen in…

Thiamine (Vitamin B1) is one of the essential vitamins and is required for carbohydrate metabolism. Thiamine hydrochloride is used as a food-additive to add a brothy/meaty flavor to gravies or soups. It is also used as food supplement. Used in fluorometric determination of mercury.

MAO inhibitor; inhibits platelet aggregation.

Potent phenolic antioxidant found in grapes and red wine. Eicosanoid synthesis and platelet aggregation inhibitor. Estrogen receptor agonist. Chemopreventive. Specific inhibitor of cyclooxygenase-1 (COX-1). Anti-inflammatory. Ribonucleotide reductase and DNA synthesis. Arrests cell cycle at S/G2…

Tight-binding, reversible, highly selective inhibitor of acid proteases (aspartyl peptidases), like pepsin, gastricsin, cathepsin E and D, renin, chymosin, bacterial aspartic proteinases and HIV proteases. Does not inhibit thiol proteases, neutral proteases or serine proteases. Widely used as a…

Cellulase is used for protoplast preparations. This enzyme shows a very high filter paper decomposing activity, as well as, appreciable xylanase and hemicellulase activity. It is an effective enzyme for use with pectolyase Y-23 for plant cell wall removal. It has been used in a study to assess the…

Collagenases degrade native helical collagen fibrils. It has an important role in connective tissue metabolism and is produced by specific cells involved in repairs and remodelling processes. Collagenase is used for tissue dissociation combined with other enzymes such as elastase, trypsin,…

Protease and Phosphatase Inhibitor Cocktail contains a mixture of inhibitors and is designed to prevent protein degradation by endogenous proteases and phosphatases released during the extraction of proteins from animal cells or tissues. Application Notes: Dilute 1/10 in RIPA or Lysis Buffer…

This α-amylase product is a liquefying diastase supplied as a dry, free-flowing powder having high uniformity and excellent storage stability. The general procedure for the enzymatic liquefaction of starch is the same, regardless of the nature of the end product. The degree of conversion…

Protease Inhibitor Cocktail Kit is a broad spectrum protease inhibitor cocktail which covers both endopeptidases and exopeptidases, non-specific and specific. The cocktail consists of four major protease inhibitors: AEBSF, Sodium EDTA, Leupeptin and Pepstatin A. Protease Inhibitor Cocktail Kit…

This enzyme is useful for enzymatic determination of creatinine when coupled with creatine amidinohydrolase, sarcosine dehydrogenase or sarcosine oxidase and formaldehyde dehydrogenase in clinical analysis. Creatininase from Pseudomonas sp. is a homohexameric enzyme with a molecular mass of…

Oleoyl Coenzyme A Dipotassium Salt

Potent anticancer compound. Anti-angiogenic. VEGF, VE-cadherin phosphorylation, matrix metalloproteinase and urokinase-plasminogen activator (uPA) inhibitor. Anti-inflammatory. NF-kappaB inhibitor. Modulates chronic inflammatory diseases, such as type 2 diabetes, rheumatoid arthritis, inflammatory…

Ribonuclease belongs to pancreatic ribonuclease family. RNase A is an endoribonuclease that attacks at the 3¢ phosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG. Ribonuclease A is used to remove RNA from DNA plasmid preparations…

Ethylenediamine Tetraacetic Acid is a polyamino carboxylic acid hexadentate ligand and a chelating agent. Ethylenediamine Tetraacetic Acid is a chelator of divalent cations. It also inhibits enzymes, such as metalloproteases, that require divalent cations for activity. Zinc-dependent…

α-Thrombin is a trypsin-like serine protease involved in a multitude of processes in the human body. Thrombin generation is the result of limited proteolysis of the vitamin K-dependent zymogen prothrombin. Thrombin is the last enzyme in the clotting cascade functioning to cleave fibrinogen to…

Choline oxidase (EC 1.1.3.17) is an enzyme that catalyzes the chemical reaction Choline + O2 betaine aldehyde + H2O2. Thus, the two substrates of this enzyme are choline and O2, whereas its two products are betaine aldehyde and H2O2. This enzyme belongs to the family of oxidoreductases,…

HRP is a plant glycohemoprotein that belongs to the ferroprotoporphyrin group of peroxidases. HRP is composed of seven isozymes. All isozymes contain photohemin IX as prosthetic group. Neutral and amino sugars account for approximately 18% of the enzyme. The iron-containing hemin group is…

Glucose-6-phosphate dehydrogenase (G-6-PDH) was used as a model to test the effect of seed protein fractions on enzyme protection during dehydration. G-6-PDH has been utilized in assays for nicotinamide adenine dinucleotide and tissue pyridine nucleotides.