DNA Modification Enzymes

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DNA Modification Enzymes

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  • illustra ExoProStar

    GE Healthcare

    Enzymatic PCR clean up technology with illustra Exonuclease I and Alkaline Phosphatase to remove unincorporated primers and dNTPs. For maximum flexibility comes in two separate tubes. Enzymes optimized to work together for high efficiency removal of unincorporated primers and nucleotides. …

  • illustra Shrimp Alkaline Phosphatase

    GE Healthcare

    Alkaline Phosphatase catalyzes the removal of 5'- and 3'-phosphate groups from DNA, RNA, nucleotides, and proteins. Bacterial alkaline phosphatase recombinantly expressed in E. coli . Active in PCR and restriction endonuclease buffers. Inactivated by heating at 75°C for 5 minutes. Bacterial…

  • illustra Exonuclease I

    GE Healthcare

    Exonuclease I catalyzes the sequential removal of nucleotides from the 3' end of single-stranded DNA molecules. 3'?5' single-stranded exonuclease. Monomeric 54.5 kDa bacterial exonuclease I recombinantly expressed in E. coli . Inactivated by heating at 80°C for 15 minutes. Active in PCR…

  • illustra ExoProStar S

    GE Healthcare

    Enzymatic PCR clean up technology with Exonuclease I and Shrimp Alkaline Phosphatase to remove unincorporated primers and dNTPs. For maximum flexibility comes in two separate tubes. Enzymes optimized to work together for high efficiency removal of unincorporated primers and nucleotides Enzymes…

  • Fast DNA Ligation Kit

    Bio Basic Inc.

    Suitable for cohesive or blunt ends and all common ligation reactions.

  • A-Tailing Kit

    Bio Basic Inc.

    To clone blunt-ended PCR fragments generated by  Pfu DNA polymerase or other enzymes to T-Vector,  A-tailing reaction is required. This kit provides Taq  DNA polymerase and dATP mix for adding single A  to these blunt-ended DNA fragments. A tailed DNA  fragments are…

  • T-Tailing Kit

    Bio Basic Inc.

    This kit is designed to add a single deoxythymidine (dT) residue at the 3’ end of any blunt-end DNA fragments (normally a vector) based on the non-template-dependent terminal transferase activity of Taq polymerase. This T residue on the vector will hybridize to the single A overhang on the…

  • Taq Plus DNA Polymerase

    Bio Basic Inc.

    Taq Plus is a mixture of Taq and Proof Reading enzyme. Taq is a thermostable DNA polymerase isolated from a strain of Thermus sp (see product number D0089). Taq Plus is used to improve the reliability and yield of conventional primer extension reaction. Taq Plus has two following advantages: (1)…

  • T4 DNA Ligase with PEG

    Bio Basic Inc.

    T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5’ phosphate and 3’ hydroxyl termini in duplex DNA or RNA. This enzyme will join blunt-end and cohesive end termini as well as repair single stranded nicks in duplex DNA, RNA, or DNA/RNA hybrids.

  • T4 DNA Ligase

    Bio Basic Inc.

    T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5’-phosphate and 3’-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single strand nicks in duplex DNA, RNA or DNA/RNA hybrids, joins DNA fragments with either cohesive or blunt termini. The T4 DNA Ligase…

  • Tsg Plus DNA Polymerase

    Bio Basic Inc.

    Tsg Plus is a mixture of Tsg and Proof reading enzyme. Tsg is a thermostable DNA polymerase isolated from a strain of Thermus sp (see product number D0081). Tsg Plus is used to improve the reliability and yield of conventional primer extension reaction.

  • Tsg DNA Polymerase

    Bio Basic Inc.

    Tsg DNA Polymerase is a thermostable DNA Polymerase isolated from a strain of Thermus sp. Tsg has a half life of 3 hours at 95C, it is therefore more stable than Tsg DNA Polymerase. Tsg has high fidelity with an error frequency 10 / 106 (or 0.01 / 103) during DNA synthesis. Tsg is designed for use…

  • T4 DNA Polymerase

    Bio Basic Inc.

    T4 DNA Polymerase, a template-depended DNA polymerase, catalyzes 5'?3' synthesis from primed single-stranded DNA. The enzyme has a 3'?5' exonuclease activity, but lacks 5'?3' exonuclease activity.

  • T4 RNA Ligase

    Bio Basic Inc.

  • DNA Polymerase I

    Bio Basic Inc.

    DNA Polymerase I, a template-dependent DNA polymerase, catalyzes 5' 3' synthesis of DNA. The enzyme also exhibits 3' 5' exonuclease (proofreading) activity, 5' 3' exonuclease activity and ribonuclease H activity.

  • phi29 DNA Polymerase

    Bio Basic Inc.

    phi29 DNA Polymerase is a highly processive polymerase (up to more than 70 kb) featuring strong strand displacement activity which allows for highly efficient isothermal DNA amplification. phi29 DNA Polymerase also possesses a 3’ ?5’ exonuclease (proofreading) activity acting preferentially on…

  • T7 RNA Polymerase

    Bio Basic Inc.

    Bacteriophage T7 RNA Polymerase is a DNAdependent RNA polymerase with strict specificity for its respective double-stranded promoter. The enzyme catalyzes the 5’?3’ synthesis of RNA on either single-stranded DNA or double-stranded DNA downstream from the promoter. T7 RNA Polymerase accepts modified…

  • Ready-To-Go T4 DNA Ligase

    GE Healthcare

    Ready-To-Go T4 DNA Ligase For covalently-linking DNA fragments in as little as 30 min. Single-dose, ambient-temperature-stable reaction mixes. For covalently-linking DNA fragments in as little as 30 min.Single-dose, ambient-temperature-stable reaction mixes.Ready-To-Go T4 DNA Ligase Catalyzes…

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