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DNA Modification Enzymes

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  • Fast DNA Ligation Kit

    Bio Basic Inc.

    Suitable for cohesive or blunt ends and all common ligation reactions.

  • illustra™ ExoProStar™ S

    GE Healthcare

    ExoProStar S is optimized to purify PCR and sequencing setup reactions quickly, efficiently, and reliably. ExoProStar S contains illustra Shrimp Alkaline phosphatase and Exonuclease A, formulated to work together to remove unincorporated primers and nucleotides from amplification reactions in…

  • T4 RNA Ligase

    Bio Basic Inc.

  • Shrimp Alkaline Phosphatase

    GE Healthcare

    A high specific activity, heat-labile alkaline phosphatase. Completely and irreversibly inactivated in Tris buffers at pH 8.0 to 8.5 by heating for 15 min at 65°C. No further treatment is necessary. Removing 5'-phosphates from DNA and RNA Easily inactivated by heat A high…

  • T7 RNA Polymerase

    Bio Basic Inc.

    Bacteriophage T7 RNA Polymerase is a DNAdependent RNA polymerase with strict specificity for its respective double-stranded promoter. The enzyme catalyzes the 5’?3’ synthesis of RNA on either single-stranded DNA or double-stranded DNA downstream from the promoter. T7 RNA Polymerase accepts modified…

  • T4 DNA Ligase

    Bio Basic Inc.

    T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5’-phosphate and 3’-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single strand nicks in duplex DNA, RNA or DNA/RNA hybrids, joins DNA fragments with either cohesive or blunt termini. The T4 DNA Ligase…

  • Ready-To-Go T4 DNA Ligase

    GE Healthcare

    For covalently-linking DNA fragments in as little as 30 min. Single-dose, ambient-temperature-stable reaction mixes Catalyzes the formation of a phosphodiester bond between the 5'-phosphoryl group and the 3'-hydroxyl group of two double-stranded DNA fragments. ATP is required for this…

  • Tsg Plus DNA Polymerase

    Bio Basic Inc.

    Tsg Plus is a mixture of Tsg and Proof reading enzyme. Tsg is a thermostable DNA polymerase isolated from a strain of Thermus sp (see product number D0081). Tsg Plus is used to improve the reliability and yield of conventional primer extension reaction.

  • DNA Polymerase I

    Bio Basic Inc.

    DNA Polymerase I, a template-dependent DNA polymerase, catalyzes 5' 3' synthesis of DNA. The enzyme also exhibits 3' 5' exonuclease (proofreading) activity, 5' 3' exonuclease activity and ribonuclease H activity.

  • Exonuclease I

    GE Healthcare

    Eliminating residual single-stranded DNA containing a 3'-terminus Measuring endonucleolytic cleavage of covalently closed circular (ccc) ssDNA Measuring DNA helicase activity Acts specifically on single-stranded DNA degrading it processively in the 3'- to 5'-direction,…

  • phi29 DNA Polymerase

    Bio Basic Inc.

    phi29 DNA Polymerase is a highly processive polymerase (up to more than 70 kb) featuring strong strand displacement activity which allows for highly efficient isothermal DNA amplification. phi29 DNA Polymerase also possesses a 3’ ?5’ exonuclease (proofreading) activity acting preferentially on…

  • A-Tailing Kit

    Bio Basic Inc.

    To clone blunt-ended PCR fragments generated by  Pfu DNA polymerase or other enzymes to T-Vector,  A-tailing reaction is required. This kit provides Taq  DNA polymerase and dATP mix for adding single A  to these blunt-ended DNA fragments. A tailed DNA  fragments are…

  • T-Tailing Kit

    Bio Basic Inc.

    This kit is designed to add a single deoxythymidine (dT) residue at the 3’ end of any blunt-end DNA fragments (normally a vector) based on the non-template-dependent terminal transferase activity of Taq polymerase. This T residue on the vector will hybridize to the single A overhang on the…

  • illustra ExoProStar

    GE Healthcare

    Enzymatic PCR clean up technology with illustra Exonuclease I and Alkaline Phosphatase to remove unincorporated primers and dNTPs. For maximum flexibility comes in two separate tubes. Enzymes optimized to work together for high efficiency removal of unincorporated primers and nucleotides …

  • Tsg DNA Polymerase

    Bio Basic Inc.

    Tsg DNA Polymerase is a thermostable DNA Polymerase isolated from a strain of Thermus sp. Tsg has a half life of 3 hours at 95C, it is therefore more stable than Tsg DNA Polymerase. Tsg has high fidelity with an error frequency 10 / 106 (or 0.01 / 103) during DNA synthesis. Tsg is designed for use…

  • T4 DNA Ligase with PEG

    Bio Basic Inc.

    T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5’ phosphate and 3’ hydroxyl termini in duplex DNA or RNA. This enzyme will join blunt-end and cohesive end termini as well as repair single stranded nicks in duplex DNA, RNA, or DNA/RNA hybrids.

  • Taq Plus DNA Polymerase

    Bio Basic Inc.

    Taq Plus is a mixture of Taq and Proof Reading enzyme. Taq is a thermostable DNA polymerase isolated from a strain of Thermus sp (see product number D0089). Taq Plus is used to improve the reliability and yield of conventional primer extension reaction. Taq Plus has two following advantages: (1)…

  • T4 DNA Polymerase

    Bio Basic Inc.

    T4 DNA Polymerase, a template-depended DNA polymerase, catalyzes 5'?3' synthesis from primed single-stranded DNA. The enzyme has a 3'?5' exonuclease activity, but lacks 5'?3' exonuclease activity.

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