Cloning and Expression

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Cloning and Expression

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  • Culture Cylinders

    Bioptechs

    A borosilicate glass cylinder similar to a cloning ring having an optically flat polished end that hydrostatically seals to another flat optical surface so that grease is not required that is used when plating cells to position them for observation and improve plating time by restricting the amount…

  • IPTG, Isopropyl ß-D-1-thiogalactopyranoside, Ultra Pure Grade

    Teknova

    IPTG, a non-metabolizable galactose analog, is commonly used in cloning procedures that require induction of β-galactosidase activity. IPTG is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in…

  • X-Gal

    MP Biomedicals

    Molecular biology grade RNase, DNase and Protease free X-Gal (5-Bromo-4-Chloro-3-Indolyl-β -D-galactopyranoside) is a β -galactosidase chromogenic substrate. In the presence of ß-galactosidase, X-Gal is hydrolyzed and yields a blue precipitate. X-Gal is used for the identification and…

  • Single Step Ultra Competent Cell Preps Kit

    Bio Basic Inc.

    This Kit contains SSCS solution, enables researchers to prepare  competent E. coli cells in a single step and to transform the cells  without heat-shock. SSCS has been reported to be faster and  easier than other methods of producing competent cells, such as  the traditional…

  • PCR Kit With DFS Taq Polymerase

    Amresco

    DNA amplification kit recommended for optimization of PCR reactions. Each kit includes 10 mM of each dNTP, 50X Reaction Buffer with MgCl2, 10X Reaction Buffer without MgCl2, 25 mM MgCl2, Taq DNA Polymerase 500U, Sterile Ultrapure Water, 2 x 1.5 ml. Templates and primers are supplied by users.…

  • E. cloni® EXPRESS BL21(DE3) Competent Cells

    Lucigen

    Lucigen’s E. cloni EXPRESS BL21(DE3) Electrocompetent Cells are the first to offer high efficiency cloning and high level protein expression in the same cell.

  • Yeast RPM Kit

    MP Biomedicals

    Plasmid DNA isolated from yeast or bacterial cells in ≤30 minutes Proven GENECLEAN® GLASSMILK® technology No phenol/chloroform extractions No ethanol precipitation The Yeast RPM Kit allows isolation of plasmid DNA from both yeast and bacterial cells. It uses a GLASSMILK purification…

  • Isopropyl-ß-D-Thiogalactopyranoside

    MP Biomedicals

    IPTG (Isopropyl-β-D-thiogalactopyranoside) is a galactose analogue not recognized by β-galactosidase. IPTG is a non-metabolizable galactose analog that induces expression of the lac operon in Escherichia coli. IPTG is a galactose analogue not recognized by β-galactosidase. It…

  • Sob & Soc Media

    MP Biomedicals

    SOB Medium is the standard media for preparing cells for chemical transformation.  SOC is a special medium for incubating competent cells immediately after transformation to allow expression of transferred resistance genes before exposing cells to selective conditions.

  • PUCM-T Cloning Vector Kit

    Bio Basic Inc.

    The T-Vector PCR Product Cloning Kit is suitable for cloning of PCR products with additional A at 3’ end. The special ligation system provided by the kit enables customs to finish ligation in 1-2 hour.

  • EZ-Yeast Transformation Kit, 200 Preps

    MP Biomedicals

    High throughput transformation of yeast cells Simple and fast No need to make competent cells The EZ-Yeast Transformation Kit is designed for high throughput transformation with an efficiency of up to 1,000 colonies per µg plasmid DNA. Suitable for a quick analysis of potentially positive…

  • Seamless Cloning Master Mix

    Bio Basic Inc.

    The Seamless Cloning Master Mix is a ready-to-use reaction mix for seamless cloning reactions. It allows for successful assembly of multiple DNA fragments, regardless of fragment length or end compatibility. It has been rapidly adopted by the synthetic biology community due to its ease-of-use,…

  • Lysozyme, 5 g

    MP Biomedicals

    Anti-microbial activity This enzyme purified from chicken egg white is provided as an albumin-free freeze dried powder in the chloride form. The enzyme catalyzes the hydrolysis of 1,4-beta-linkages between Nacetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between…

  • Phage Display Competent Cells

    Lucigen

    SS320 Electrocompetent Cells . A non-amber suppressor strain (sometimes called MC1061F') prepared as highly efficient (≥4 x 10 10 cfu/µg) electrocompetent cells for phage display library screening. These cells have the highest available transformation efficiency of any strain used for…

  • Endura™ Competent Cells

    Lucigen

    Clone unstable sequences with reliable results Direct replacement for Invitrogen Stbl3™ and Agilent SURE strain Available as chemically competent or electrocompetent cells High efficiency: over 1 x 10 7 cfu/µg (chem) or 1 x 10 10 cfu/µg (electro) Ideal for CRISPR…

  • Recovery Medium

    Lucigen

    Recommended for all competent cell transformations Enables the highest possible transformation efficiency Use in the outgrowth stage of competent cell transformation to achieve 10-folder higher transformation efficiency than you would get with LB or SOC media Trusted formulation used in…

  • PUCM-T Cloning Vector

    Bio Basic Inc.

  • Yeast Cell Lysis Kit, 100 Preps

    MP Biomedicals

    The Yeast Cell Lysis Kit contains the reagents necessary for the yeast cell wall in preparation for efficient lysis with the GNOME® DNA Isolation Kit. It can also be used prior to other traditional DNA purification kits in which reagents alone are not sufficient to lyse yeast cell walls.

  • Alkali Cation Yeast Transformation Kit, 250 Transformations

    MP Biomedicals

    High efficiency transformation of yeast cells Process takes less than 90 minutes The Alkali Cation Yeast transformation kit provides all the reagents required to perform a high efficiency transformation of yeast with circular or linear DNA. The entire procedure can be completed in one day and…

  • Super-Comp Media And Transformation Salts

    MP Biomedicals

    SUPER-COMP is an extremely rich medium that significantly increases the competency of the cells when using a simple RbCl/CaCl means of preparing competent host cells. After cells are made competent, they are immediately ready for DNA uptake. For maximum transformation efficiency, store for…

  • CJ236 Electrocompetent Cells

    Lucigen

    High efficiency cells to create uracil-containing DNA for site-directed mutagenesis Sole source of highly efficient Electrocompetent cells (1 x 10 9 cfu/µg) ung- and dut- mutations to generate DNA with some uracil residues Ideal genotype and efficiency for Kunkel directed…

  • Novagen® Rosetta™ 2 Competent Cells

    MilliporeSigma

    Rosetta™ 2 host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. These strains supply tRNAs for 7 rare codones (AGA, AGG, AUA, CUA, GGA, CCC, and CGG) on a compatible chloramphenicol-resistant plasmid. The tRNA…

  • BigEasy®-TSA™ Electrocompetent Cells

    Lucigen

    Cells required for transformation and propagation of pJAZZ® Vectors Highest available transformation efficiencies for library construction and screening Optimized for high yields of DNA

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