Tris is compatible with many enzymes in molecular biology, for example DNA modifying enzymes, because its buffering range is between 7.1-8.9. The lower pH 7.4 can be associated more with maintaining RNA, however, the pH 8.0 works equally as well with both DNA and RNA nucleic acids. Applications…

Kanamycin sulfate is a water soluble broad spectrum antibiotic obtained from soil bacterium Streptomyces kanamyceticus. It is an aminoglycoside antibiotic effective against Gram-negative and Gram-positive bacteria and mycoplasma. It binds to the 30S ribosomal subunit, inhibits translocation, and…

Proprietary Sheath Fluid Prepared using water deionized by reverse osmosis 1µm sterile filtered Gamma irradiated to a minimum of 25kGy and maximum of 50kGy Applications Designed specifically for flow cytometry applications.

PBS (phosphate buffered saline) is a balanced salt solution used for a variety biological and cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. Endotoxin and Mycoplasma tested Applications …

DPBS is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. Calcium and magnesium facilitate cell binding and clumping. DPBS without these ions can be used to wash and rinse suspended cells. Without Phenol Red Applications DPBS can be used to provide a…

TBE (TRIS Borate EDTA) is often used in procedures involving nucleic acids, the most common being electrophoresis. Tris-acid solutions are effective buffers for slightly basic conditions, which keep DNA deprotonated and soluble in water. Applications EDTA is a chelator of divalent cations,…

Sodium Bicarbonate (NaHCO3) is typically used in cell culture media as a buffer. Endotoxin and Mycoplasma Tested Applications Although NaHCO3 does not have the buffering capacity of HEPES, it is occasionally preferred over HEPES because of its lower toxicity and nutritional value to cells…

DPBS is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. Calcium and magnesium facilitate cell binding and clumping. DPBS without these ions can be used to wash and rinse suspended cells. Without Phenol red Applications DPBS can be used to provide a…

Tris HCl, 10X pH 7.2 is a Buffered Saline Solution used in procedures for the isolation of DNA. Filtered through a 0.22 µm membrane DNase, RNase, and Protease tested Applications Tris buffered saline is useful for the following: as a biological diluent buffer, as a buffer for antibodies,…

Tris HCl 2 M pH 6.8 is useful with SDS-PAGE and Electrophoresis. Tris solutions are formulated into working concentrations for preparing the stacking and resolving portions of native or SDS-PAGE gels using discontinuous buffer systems according to Laemmli (1970) or Ornstein and Davis (1959). …

TE is composed of buffering agent Tris, and chelating agent EDTA. EDTA prevents degradation of DNA and RNA by chelating magnesium (or other divalent metal ions). Together, this Tris-EDTA based solution solubilizes DNA or RNA, while protecting it from degradation. Filtered through a 0.22 µm…

L-glutamine is a cell culture supplement, and an essential amnio acid. L-glutamine is required in cell culture for cellular growth. 100X concentration Applications L-glutamine is used in cell culture as an energy source in addition to carbohydrates (i.e. glucose, sucrose, maltose, etc.).…

DPBS is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. The lack of calcium and magnesium allows DPBS to be used to wash and rinse suspended cells. Without Phenol Red Applications DPBS can be used to provide a buffer system to maintain cell culture…